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On-line Access: 2023-07-12
Received: 2022-12-19
Revision Accepted: 2023-06-14
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3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels
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Zhuang Chen, Chuanzhen Huang, Hanlian Liu, Xu Han, Zhichao Wang, Shuying Li, Jun Huang & Zhen Wang . 3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels[J]. Journal of Zhejiang University Science D, 2023, 6(5): 550–562.
@article{title="3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels",
author="Zhuang Chen, Chuanzhen Huang, Hanlian Liu, Xu Han, Zhichao Wang, Shuying Li, Jun Huang & Zhen Wang ",
journal="Journal of Zhejiang University Science D",
volume="6",
number="5",
pages="550–562",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00251-5"
}
%0 Journal Article
%T 3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels
%A Zhuang Chen
%A Chuanzhen Huang
%A Hanlian Liu
%A Xu Han
%A Zhichao Wang
%A Shuying Li
%A Jun Huang & Zhen Wang
%J Journal of Zhejiang University SCIENCE D
%V 6
%N 5
%P 550–562
%@ 1869-1951
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00251-5
TY - JOUR
T1 - 3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels
A1 - Zhuang Chen
A1 - Chuanzhen Huang
A1 - Hanlian Liu
A1 - Xu Han
A1 - Zhichao Wang
A1 - Shuying Li
A1 - Jun Huang & Zhen Wang
J0 - Journal of Zhejiang University Science D
VL - 6
IS - 5
SP - 550–562
EP -
%@ 1869-1951
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00251-5
Abstract: Three-dimensional (3D) bioprinting has been used widely for the construction of hard tissues such as bone and cartilage. However, constructing soft tissues with complex structures remains a challenge. In this study, complex structures characterized by both tunable elastic modulus and porosity were printed using freeform reversible embedding of suspended hydrogels (FRESHs) printing methods. A mixture of alginate and gelatin was used as the main functional component of the bioink. Rheological analysis showed that this bioink possesses shear thinning and shear recovery properties, supporting both cryogenic and FRESH printing methods. Potential printing capabilities and limitations of cryogenic and FRESH printing were then analyzed by printability tests. A series of complex structures were printed by FRESH printing methods which could not be realized using conventional approaches. Mechanical tests and scanning electron microscopy analysis showed that the printed structure is of excellent flexibility and could be applied in various conditions by adjusting its mechanical modulus and porosity. L929 fibroblast cells maintained cell viability in cell-laden-printed structures, and the addition of collagen further improved the hydrogels’ biocompatibility. Overall, all results provided useful insight into the building of human soft tissue organ blocks.
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