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Abstract: This study investigated whether four cumulus-specific genes: follicular stimulating hormone receptor (FSHr), hyaluronan synthase 2 (Has2), prostaglandin synthase 2 (Ptgs2) and steroidogenic acute regulator protein (Star), were correctly reprogrammed to be transcriptionally silent following somatic cell nuclear transfer (SCNT) in a murine model. Cumulus cells of C57×CBA F1 female mouse were injected into enucleated oocytes, followed by activation in 10 µmol/L strontium chloride for 5 h and subsequent in vitro culture up to the blastocyst stage. Expression of cumulus-specific genes in SCNT-derived embryos at 2-cell, 4-cell and day 4.5 blastocyst stages was compared with corresponding in vivo fertilized embryos by real-time PCR. It was demonstrated that immediately after the first cell cycle, SCNT-derived 2-cell stage embryos did not express all four cumulus-specific genes, which continually remained silent at the 4-cell and blastocyst stages. It is therefore concluded that all four cumulus-specific genes were correctly reprogrammed to be silent following nuclear transfer with cumulus donor cells in the mouse model. This would imply that the poor preimplantation developmental competence of SCNT embryos derived from cumulus cells is due to incomplete reprogramming of other embryonic genes, rather than cumulus-specific genes.

[1] Armstrong, L., Lako, M., Dean, W., Stojkovic, M., 2006. Epigenetic modification is central to genome reprogramming in somatic cell nuclear transfer. Stem. Cells, 24(4):805-814.

[2] Baguisi, A., Behboodi, E., Melican, D.T., Pollock, J.S., Destrempes, M.M., Cammuso, C., Williams, J.L., Nims, S.D., Porter, C.A., Midura, P., et al., 1999. Production of goats by somatic cell nuclear transfer. Nat. Biotechnol., 17(5):456-461.

[3] Bortvin, A., Eggan, K., Skaletsky, H., Akutsu, H., Berry, D.L., Yanagimachi, R., Page, D.C., Jaenisch, R., 2003. Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei. Development, 130(8):1673-1680.

[4] Calder, M.D., Caveney, A.N., Smith, L.C., Watson, A.J., 2003. Responsiveness of bovine cumulus-oocyte-complexes (COC) to porcine and recombinant human FSH, and the effect of COC quality on gonadotropin receptor and Cx43 marker gene mRNAs during maturation in vitro. Reprod. Biol. Endocrinol., 1(1):14.

[5] Challah-Jacques, M., Chesne, P., Renard, J.P., 2003. Production of cloned rabbits by somatic nuclear transfer. Cloning Stem. Cells, 5(4):295-299.

[6] Chatot, C.L., Ziomek, C.A., Bavister, B.D., Lewis, J.L., Torres, I., 1989. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J. Reprod. Fertil., 86(2):679-688.

[7] Cibelli, J.B., Stice, S.L., Golueke, P.J., Kane, J.J., Jerry, J., Blackwell, C., Ponce de Leon, F.A., Robl, J.M., 1998. Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat. Biotechnol., 16(7):642-646.

[8] Elvin, J.A., Clark, A.T., Wang, P., Wolfman, N.M., Matzuk, M.M., 1999. Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Mol. Endocrinol., 13(6):1035-1048.

[9] Erbach, G.T., Lawitts, J.A., Papaioannou, V.E., Biggers, J.D., 1994. Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod., 50(5):1027-1033.

[10] Fang, Z.F., Gai, H., Huang, Y.Z., Li, S.G., Chen, X.J., Shi, J.J., Wu, L., Liu, A., Xu, P., Sheng, H.Z., 2006. Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos. Exp. Cell Res., 312(18):3669-3682.

[11] Galli, C., Lagutina, I., Crotti, G., Colleoni, S., Turini, P., Ponderato, N., Duchi, R., Lazzari, G., 2003. Pregnancy: a cloned horse born to its dam twin. Nature, 424(6949):635.

[12] Gao, S., Chung, Y.G., Williams, J.W., Riley, J., Moley, K., Latham, K.E., 2003. Somatic cell-like features of cloned mouse embryos prepared with cultured myoblast nuclei. Biol. Reprod., 69(1):48-56.

[13] Gómez, M.C., Pope, C.E., Giraldo, A., Lyons, L.A., Harris, R.F., King, A.L., Cole, A., Godke, R.A., Dresser, B.L., 2004. Birth of African Wildcat cloned kittens born from domestic cats. Cloning Stem. Cells, 6(3):247-258.

[14] Han, Y.M., Kang, Y.K., Koo, D.B., Lee, K.K., 2003. Nuclear reprogramming of cloned embryos produced in vitro. Theriogenology, 59(1):33-44.

[15] Hinrichs, K., Choi, Y.H., Love, C.C., Chung, Y.G., Varner, D.D., 2006. Production of horse foals via direct injection of roscovitine-treated donor cells and activation by injection of sperm extract. Reproduction, 131(6):1063-1072.

[16] Hosaka, K., Ohi, S., Ando, A., Kobayashi, M., Sato, K., 2000. Cloned mice derived from somatic cell nuclei. Hum. Cell, 13(4):237-242.

[17] Kato, Y., Tani, T., Tsunoda, Y., 2000. Cloning of calves from various somatic cell types of male and female adult, newborn and fetal cows. J. Reprod. Fertil., 120(2):231-237.

[18] Lanza, R.P., Cibelli, J.B., Diaz, F., Moraes, C.T., Farin, P.W., Farin, C.E., Hammer, C.J., West, M.D., Damiani, P., 2000. Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning, 2(2):79-90.

[19] Lee, B.C., Kim, M.K., Jang, G., Oh, H.J., Yuda, F., Kim, H.J., Shamim, M.H., Kim, J.J., Kang, S.K., Schatten, G., et al., 2005. Dogs cloned from adult somatic cells. Nature, 436(7051):641.

[20] Lee, E., Lee, S.H., Kim, S., Jeong, Y.W., Kim, J.H., Koo, O.J., Park, S.M., Hashem, M.A., Hossein, M.S., Son, H.Y., et al., 2006. Analysis of nuclear reprogramming in cloned miniature pig embryos by expression of Oct-4 and Oct-4 related genes. Biochem. Biophys. Res. Commun., 348(4):1419-1428.

[21] Li, Z., Sun, X., Chen, J., Liu, X., Wisely, S.M., Zhou, Q., Renard, J.P., Leno, G.H., Engelhardt, J.F., 2006. Cloned ferrets produced by somatic cell nuclear transfer. Dev. Biol., 293(2):439-448.

[22] Liu, J., Sirois, J., 1998. Follicle size-dependent induction of prostaglandin G/H synthase-2 during superovulation in cattle. Biol. Reprod., 58(6):1527-1532.

[23] Loi, P., Ptak, G., Barboni, B., Fulka, J.Jr, Cappai, P., Clinton, M., 2001. Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nat. Biotechnol., 19(10):962-964.

[24] Meehan, R.R., 2003. DNA methylation in animal development. Semin. Cell Dev. Biol., 14(1):53-65.

[25] Polejaeva, I.A., Chen, S.H., Vaught, T.D., Page, R.L., Mullins, J., Ball, S., Dai, Y., Boone, J., Walker, S., Ayares, D.L., et al., 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature, 407(6800):86-90.

[26] Prather, R.S., Sutovsky, P., Green, J.A., 2004. Nuclear remodeling and reprogramming in transgenic pig production. Exp. Biol. Med. (Maywood), 229(11):1120-1126.

[27] Sansinena, M.J., Hylan, D., Hebert, K., Denniston, R.S., Godke, R.A., 2005. Banteng (Bos javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenology, 63(4):1081-1091.

[28] Santos, F., Dean, W., 2004. Epigenetic reprogramming during early development in mammals. Reproduction, 127(6):643-651.

[29] Shimada, M., Hernandez-Gonzalez, I., Gonzalez-Robayna, I., Richards, J.S., 2006. Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor. Mol. Endocrinol., 20(6):1352-1365.

[30] Smillie, D.A., Llinas, A.J., Ryan, J.T., Kemp, G.D., Sommerville, J., 2004. Nuclear import and activity of histone deacetylase in Xenopus oocytes is regulated by phosphorylation. J. Cell Sci., 117(9):1857-1866.

[31] Thibault, C., 2003. Recent data on the development of cloned embryos derived from reconstructed eggs with adult cells. Reprod. Nutr. Dev., 43(4):303-324.

[32] Tong, G.Q., Heng, B.C., Tan, L.G., Ng, S.C., 2006. Aberrant profile of gene expression in cloned mouse embryos derived from donor cumulus nuclei. Cell Tissue Res., 325(2):231-243.

[33] Valenzuela, C.Y., 2005. The risk of congenital malformations and genomic imprinting defects in assisted reproductive technologies and nuclear transfer cloning. Rev. Med. Chil., 133(9):1075-1080.

[34] Wakayama, T., 2003. Cloned mice and embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Oncol. Res., 13(6-10):309-314.

[35] Wilmut, I., Schnieke, A.E., McWhir, J., Kind, A.J., Campbell, K.H., 1997. Viable offspring derived from fetal and adult mammalian cells. Nature, 385(6619):810-813.

[36] Yamazaki, Y., Fujita, T.C., Low, E.W., Alarcon, V.B., Yanagimachi, R., Marikawa, Y., 2006. Gradual DNA demethylation of the Oct4 promoter in cloned mouse embryos. Mol. Reprod. Dev., 73(2):180-188.

[37] Zhou, Q., Renard, J.P., Le Friec, G., Brochard, V., Beaujean, N., Cherifi, Y., Fraichard, A., Cozzi, J., 2003. Generation of fertile cloned rats by regulating oocyte activation. Science, 302(5648):1179.