CLC number: S432.41
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 27
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ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li. cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus[J]. Journal of Zhejiang University Science A, 2002, 3(2): 216-222.
@article{title="cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus",
author="ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li",
journal="Journal of Zhejiang University Science A",
volume="3",
number="2",
pages="216-222",
year="2002",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2002.0216"
}
%0 Journal Article
%T cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus
%A ZHANG Heng-mu
%A CHEN Jian-ping
%A XUE Qing-zhong
%A LEI Juan-li
%J Journal of Zhejiang University SCIENCE A
%V 3
%N 2
%P 216-222
%@ 1869-1951
%D 2002
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2002.0216
TY - JOUR
T1 - cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus
A1 - ZHANG Heng-mu
A1 - CHEN Jian-ping
A1 - XUE Qing-zhong
A1 - LEI Juan-li
J0 - Journal of Zhejiang University Science A
VL - 3
IS - 2
SP - 216
EP - 222
%@ 1869-1951
Y1 - 2002
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2002.0216
Abstract: Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).
[1] Anzola, J. V., Xu, Z., Asamizu, T. et al.,1987. Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumour virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, USA. 84: 8301-8305.
[2] Azuhata,F., Uyeda,I., Kimura,I.et al., 1993. Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. J Gen Virol. 74: 1227-1232.
[3] Boccardo, G., Milne,R.G. (1984). Plant reovirus group. CMI/AAB, Descriptions of Plant Viruses No. 294, Association of Applied Biologists, Wellesbourne, Warwick.
[4] Genetics Computer Group, 1998. Wisconsin Package version 10.0. 575 Science Drive, Madison, Wisconsin, USA 53711.
[5] Isogai, M., Azuhata, F., Uyeda, I. et al., 1995. Genomic relationships between rice black-streaked dwarf and maize rough dwarf Fijiviruses detected by nucleic acid hybridization. Ann Phytopath Soc Japan. 61: 513-518.
[6] Isogai,M., Uyedam,I., Lee,B-C.,1998. Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9, and S10. J Gen Virol. 79: 1487-1494.
[7] Li, Y., Xu, H., Cheng, M. F., et al.,1998. Functional genomic analysis of rice dwarf virus. Acta Scientiarum Naturalium Universitis Pekinensis. 34(2-3):332-341.
[8] Luisoni, E., Lovisolo, O., Kitagawa, Y. et al.,1973. Serological relationship between maize rough dwarf virus and rice black streaked dwarf virus. Virology. 52: 281-283.
[9] Marzachi, C., Boccardo, G., Milne, R., et al.1995. Genome structure and variability of fijiviruses. Sem Virol. 6: 103-108.
[10] Sambrook,J., Fritsch, E. F., Maniatis,T.,1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
[11] Shikata,E., Kitagawa,Y.,1977. Rice black-steaked dwarf virus: its properties, morphology, and intracellular localization. Virology. 77: 826-842.
[12] Uyeda, I., Bong-Choon, L., Ando, Y.1998. Reovirus isolation and RNA extraction. In: Foster GD and Taylor SC (eds.), Methods in Molecular Biology, Vol. 81. Plant virology protocols: From virus isolation to transgenic resistance. Humana Press, Totowa, New Jersey, p.65-75.
[13] Van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L., et al., (eds), 2000. Virus Taxonomy, In: Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, New York and San Diego.
[14] Zhang,H.M., Lei,J.L., Chen,J.P.,2000. A dwarf disease on rice, wheat and maize in different region of China is caused by rice black-streaked dwarf virus (RBSDV). In: Zhou GH and Li HF.(eds.), Proceedings of the First Asian Conference on Plant Pathology. China Agricultural Scientech Press, Beijing, China, p.155.
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