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Received: 2001-07-18

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Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.2 P.216-222


cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus

Author(s):  ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li

Affiliation(s):  College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   jpchen@mail.hz.zj.cn

Key Words:  Rice black-streaked dwarf virus(RBSDV), Genome segment S8, Sequence analysis, Biotechnology

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ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li. cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus[J]. Journal of Zhejiang University Science A, 2002, 3(2): 216-222.

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Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).

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