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Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.5 P.606-610

http://doi.org/10.1631/jzus.2002.0606


Preparation of protein samples for gel electrophoresis by sequential extraction


Author(s):  ZHONG Bo-xiong, WENG Hong-biao, FANG Wei-huan

Affiliation(s):  College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   bzhong@mail.hz.zj.cn

Key Words:  Proteome, Sequential extraction, 2D-electrophoresis, Protein spot match, Amino acid sequence


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ZHONG Bo-xiong, WENG Hong-biao, FANG Wei-huan. Preparation of protein samples for gel electrophoresis by sequential extraction[J]. Journal of Zhejiang University Science A, 2002, 3(5): 606-610.

@article{title="Preparation of protein samples for gel electrophoresis by sequential extraction",
author="ZHONG Bo-xiong, WENG Hong-biao, FANG Wei-huan",
journal="Journal of Zhejiang University Science A",
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pages="606-610",
year="2002",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2002.0606"
}

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T1 - Preparation of protein samples for gel electrophoresis by sequential extraction
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2002.0606


Abstract: 
Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm, Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about 15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with the sequential extraction method; which will be useful for preparation of protein samples for proteome study.

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Reference

[1] Chevallet, M., Santoni, V., Poinas, A. Rouquie, D., Fuchs, A., Kieffer, S., Rossignol, M., Lunardi, J., Garin, J., Rabilloud, T., 1998. New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis. Electrophoresis, 19:1901-1909.

[2] Fialka, I., Pasquali, C., Lottspeich, F., Ahorn, H., Huber, L.A., 1997. Subcellular fractionation of polarized epithelial cells and identification of organelle-specific proteins by twe-dimensional gel electrophoresis. Electrophoresis, 18:2582-2590.

[3] Gianazza, E., Rabilloud, T., Quaglia, L. Caccia, P., Astrua-Testori, S., Osio, L., Grazioli, G., Righetti, P.G., 1987. Additives for immobilized pH gradient two-dimensional secaration of particulate material: Comparison between commercial and new synthetic detergents. Anal. Biochem., 165:247-257.

[4] Molloy, M. P., Herbert, B. R., Walsh, B. J., Tyler, M.I., Traini, M., Sanchez, J.C., Hochstrasser, D.F., Williams, K.L., Gooley, A.A., 1998. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis, 19:837-844.

[5] Molloy, M. P., Herbert, B. R., Williams, K. L., Gooley, A.A., 1999. Extraction of Escherichia coli proteins with organic solvents prior to twe-dimensional electrophoresis. Electrophoresis, 20:701-704.

[6] Pasquali, C., Fialka, I., Huber, L. A. 1997. Preparative two-dimensional gel electrophoresis of membrane proteins. Electrophoresis, 18:2573-2581.

[7] Rabilloud, T., Gianazza, E., Catto, N., Righetti, P.G., 1990. Amidosulfobetaines, a family of detergents with improved solubilization properties: application for isoelectric focusing under denaturing conditions. Anal. Biochem., 185:94-102.

[8] Rabilloud, T., 1996. Solubilization of proteins for electrophoretic analyses. Electrophoresis, 17:813-829.

[9] Rabilloud, T., Adessi, C., Giraudel, A. et al., 1997. Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis, 18:307-316.

[10] Santoni V., Rabilloud, T., Doumas, P., Rouquie, D., Mansion, M., Kieffer, S., Garin, J., Rossignol, M., 1999. Towards the recovery of hydrophobic proteins on twe-dimensional electrophoresis gel. Electrophoresis, 20:705-711.

[11] Wasinger, V. C., Cordwell, S. J., Cerpa-Poljak, A., Yan, J.X., Gooley, A.A., Wilkins, M.R., Duncan, M.W., Harris, R., Williams, K.L., Humphery-Smith, I., 1995. Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium. Electrophoresis, 16:1090-1094.

[12] Zhong, B., 1997. A new method for analysis of rice protein using two-dimensional electrophoresis. J. Zhejiang Agri. Uni., 23(2):128-132 (in Chinese).

[13] Zhong, B., Karibe, H., Komatsu, S., Ichimura, H., Nagamura, Y., Sasaki, T., Hiraro, H., 1997. Screening of rice genes from a cDNA catalog based on the sequence data-file of proteins separated by twe-dimensional electrophoresis. Breeding Sci., 47(3):245-251.

[14] Zhong, B., 1999. Characterization and stage-specific change of proteins during the embryonic development of silkworm Bombyx mori. Acta Genetica Sinica, 26(6):627-633 (in Chinese).

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