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Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.5 P.606-610


Preparation of protein samples for gel electrophoresis by sequential extraction

Author(s):  ZHONG Bo-xiong, WENG Hong-biao, FANG Wei-huan

Affiliation(s):  College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   bzhong@mail.hz.zj.cn

Key Words:  Proteome, Sequential extraction, 2D-electrophoresis, Protein spot match, Amino acid sequence

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ZHONG Bo-xiong, WENG Hong-biao, FANG Wei-huan. Preparation of protein samples for gel electrophoresis by sequential extraction[J]. Journal of Zhejiang University Science A, 2002, 3(5): 606-610.

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T1 - Preparation of protein samples for gel electrophoresis by sequential extraction
A1 - ZHONG Bo-xiong
A1 - WENG Hong-biao
A1 - FANG Wei-huan
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DOI - 10.1631/jzus.2002.0606

Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm, Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about 15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with the sequential extraction method; which will be useful for preparation of protein samples for proteome study.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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