CLC number: Q939
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Received: 2002-05-29
Revision Accepted: 2003-01-24
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HE Guo-qing, CHEN Qi-he, JU Xiao-jie, SHI Nai-dong. Improved elastase production by Bacillus sp. EL31410 — further optimization and kinetics studies of culture medium for batch fermentation[J]. Journal of Zhejiang University Science A, 2004, 5(2): 149-156.
@article{title="Improved elastase production by Bacillus sp. EL31410 — further optimization and kinetics studies of culture medium for batch fermentation",
author="HE Guo-qing, CHEN Qi-he, JU Xiao-jie, SHI Nai-dong",
journal="Journal of Zhejiang University Science A",
volume="5",
number="2",
pages="149-156",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0149"
}
%0 Journal Article
%T Improved elastase production by Bacillus sp. EL31410 — further optimization and kinetics studies of culture medium for batch fermentation
%A HE Guo-qing
%A CHEN Qi-he
%A JU Xiao-jie
%A SHI Nai-dong
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 2
%P 149-156
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0149
TY - JOUR
T1 - Improved elastase production by Bacillus sp. EL31410 — further optimization and kinetics studies of culture medium for batch fermentation
A1 - HE Guo-qing
A1 - CHEN Qi-he
A1 - JU Xiao-jie
A1 - SHI Nai-dong
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 2
SP - 149
EP - 156
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0149
Abstract: An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K2HPO4, MgSO4·7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K2HPO4 0.206 g/100 ml and MgSO4·7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.
[1] Box, G.E.P., Wilson, K.B., 1951. On the experimental attainment of optimum conditions.J Roy Stat Soc B,13:145.
[2] Box, G.E.P., Hunter, W.G., 1978. Statistics for Experimenters. John Wiley and Sons, NY.
[3] Chen, W.C.H., Li, C.H., 1996. Production ofβ-fructofuranosidase byAspergillus japonicus. Enzyme Microbial Technology,18:153-160.
[4] Chen, Q.H., He, G.Q., 2002. Optimization of medium composition for the production of elastase byBacillussp. EL31410 with response surface methodology.Enzyme Microbial Technology,5:67-72.
[5] Haaland, P.D., 1989. Experimental Design in Biotechnology. Dekker, New York.
[6] Hall, D.A., Czerkawaki, J.W., 1961. The reaction between elastase and elastic tissue.Biochem. J,80:128-136.
[7] Heather, M.M., Howard, G.W., Caroline, A.O., Robert, A.S., Edward, J.C., 1999. Interaction between leukocyte elastase and elastin: quantitative and catalytic analyses.Biochimica et Biophysica Acta,1430:179-190.
[8] Janda, J.M. , Sharon, L.A., 1999. Identification and Initial Characterization of elastase activity Associated withVibrio cholerae.Current Microbiol,39:73-78.
[9] Joan, C.O., Dennis, E.O., 1992. Efficient productions and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.J Bacteriology,12:4140-4147.
[10] Joelle, M.S., Florence M.C., Jean, M.W., 1989. Elastolytic activity of Pseudomonas aeruginosa elastase.Biochimica Biophysca Acta,995:285-290.
[11] King, V.A., 1993. Studies on the control of the growth ofSaccharomyces cerevisiaeby using response surface methodology to achieve effective preservation at high water activities.Int. J. Food Sci Technol,28:519-529.
[12] Khuri, A.I., Cornell, J.A., 1987. Response Surfaces Design and Analysis. Dekker, New York.
[13] Ma, A.Y.M., Ooraikul, B., 1986. Optimization of enzymatic hydrolysis of canola meal with response surface methodology.J Food Proc Preserv,10:99-113.
[14] Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar.Anal Chem,31:426-427.
[15] Morihara, K., 1967. Elastolytic properties of various proteases from microbial origin.Arch Biochem Biophys,120:68-78.
[16] Ozaki, H., Shiio, I., 1975. Purification and properties of elastolytic enzyme fromFlavobacterium immotum. J Biochem,77:171-180.
[17] Ramírez, H.G., Anne, G., 2001. Optimization of astaxanthin production by Phaffia rhodozyma through factorial design and response surface methodology.J Biotechnol,88:259-268.
[18] Robert, P.M., Thomas, J.B., Catherine, J.F., Steven, D.S., Howard, G.W., Robert, M.S., 1997. Elastin degradation by matrix metalloproteinases.J Biol Biochem,272(29):18071-18076.
[19] Roseiro, J.C., 1992. Medium development for xanthan, production.Process Biochem,27:167-175.
[20] Rosi, I., Costamagna, L., Bertuccioli, M., 1987. Wine Fermentation by Immobilized Yeast: An Optimization Study.In:Flavor Science and Technology. John Wiley and Sons, New York.
[21] Sachar, L.A., 1955. Photometry method for estimation of elastase activity.Proc Soc Expeti Biol Med,90:323-325.
[22] Sarra, M., Redin, I., Ochin, F., Godia, F., Casas, C., 1993. Application of factorial design to the optimization of medium composition in batch cultures ofStreptomyces lividansTK21 producing a hybrid antibiotic.Biotechnology Letters,15:559-564.
[23] Sharon, S.R., Jennifer, L.R., Bryan, N.B., 1997. Identification of an elastolytic protease in stationary phase culture filtrates of M.tuberculosis.FEMS Microbiology,151:59-64.
[24] Shibata, Y., Fujimura, S., Nakamura, T., 1993. Purification and partial characterization of an elastolytic serine protease ofPrevotella intermedia.Appl Environm Microbial,7:2107-2111.
[25] Shiio, I., Nakamatsu, T., Ozaki, H., 1974. Microbial production of elastolytic enzymes.Agric Biol Chem,1:1-7.
[26] Souza, M.C.de O., Roberto, I. C., 1999. Solid-state fermentation for xylanase production byThermoascus aurantiacususing response surface methodology.Appl Microbiol Biotechnol,52:768-772.
[27] Thayer, 1991. Three-dimensional structure of the elastase ofPseudomonas aeruginosaat 1.5 resolution.J Biol Chem,286:286-2871.
[28] Tsai, Y.C.H., Jung, R.Y., Lin, S.F., 1988. Production and further characterization of an alkaline elastase production by alkalophilicBacillusstrain YaB.Appl Environm Microbial,1:3156-3161.
[29] Tsuzuki, H., Oka, T., 1965.Pseudomonas aeruginosaelastase: isolation, crystallization and preliminary characterization.J Biol Chem,8:3295-3303.
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