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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.454-456

http://doi.org/10.1631/jzus.2005.B0454


Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii


Author(s):  GAO Yong-gui, YAO Min, TANAKA Isao

Affiliation(s):  Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan; more

Corresponding email(s):   gao@castor.sci.hokudai.ac.jp

Key Words:  Pyrococcus horikoshii, Methyltransferase, X-ray diffraction, Protein crystallography


GAO Yong-gui, YAO Min, TANAKA Isao. Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii[J]. Journal of Zhejiang University Science B, 2005, 6(6): 454-456.

@article{title="Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii",
author="GAO Yong-gui, YAO Min, TANAKA Isao",
journal="Journal of Zhejiang University Science B",
volume="6",
number="6",
pages="454-456",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0454"
}

%0 Journal Article
%T Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii
%A GAO Yong-gui
%A YAO Min
%A TANAKA Isao
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 6
%P 454-456
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0454

TY - JOUR
T1 - Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii
A1 - GAO Yong-gui
A1 - YAO Min
A1 - TANAKA Isao
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 6
SP - 454
EP - 456
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0454


Abstract: 
RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 predicting RNA methyltransferase from Pyrococcus horikoshii OT3 has been crystallized. The crystals of selenomethionyl PH1948 belong to space group C2, with unit-cell parameters a=207.0 Å, b=43.1 Å, c=118.2 Å, β=92.1°, and diffract X-rays to 2.2 Å resolution. The VM value was determined to be 2.8 Å3/Da, indicating the presence of four protein molecules in the asymmetric unit.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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[2] BÜgl, H., Fauman, E.B., Staker, B.L., Zheng, F., Kushner, S.R., Saper, M.A., Bardwell, J.C.A., Jakob, U., 2002. RNA methylation under heat shock control. Molecular Cell, 6:349-360.

[3] Cheng, X.D., Roberts, R.J., 2001. AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucleic Acids Research, 29:3784-3795.

[4] Chiang, P.K., Gordon, R.K., Tal, J., Zeng, G.C., Doctor, B.P., Pardhasaradhi, K., McCann, P.P., 1996. S-adenosylmethionine and methylation. FASEB J., 10:471-480.

[5] Forouhar, F., Shen, J., Xiao, R., Acton, T.B., Montelion, G., Liang, T., 2003. Functional assignment based on structural analysis: Crystal structure of the yggJ protein (HI0303) of Haemophilus influenza reveals an RNA methyltransferase with deep trefoil knot. Proteins: Structure, Function and Genetics, 53:329-332.

[6] Martin, J.L., McMillan, F.M., 2002. SAM (dependent) I AM: The S-adenosylmethionine-dependent methyltransferase fold. Current Opinion in Structure Biology, 12:783-793.

[7] Schubert, H.L., Blumenthal, R.M., Cheng, X.D., 2003. Many paths to methyltransfer: A chronicle of convergence. Trends in Biochemical Science, 28:329-335.

[8] Yeh, J.I., Hol, W.G., 1998. A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase. Acta Cryst, D54:479-480.

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