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Received: 2005-05-17

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.10 P.1015-1021


Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method

Author(s):  LI Ying-hua, LV Xiu-yang

Affiliation(s):  Institute of Pharmaceutical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   luxiuyang@zju.edu.cn

Key Words:  Shengmaiyin, 5-HMF, Origin, Schisandra, Process

LI Ying-hua, LV Xiu-yang. Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method[J]. Journal of Zhejiang University Science B, 2005, 6(10): 1015-1021.

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author="LI Ying-hua, LV Xiu-yang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method
%A LI Ying-hua
%A LV Xiu-yang
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 10
%P 1015-1021
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1015

T1 - Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method
A1 - LI Ying-hua
A1 - LV Xiu-yang
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 10
SP - 1015
EP - 1021
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1015

The origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a shengmaiyin decoction was investigated by the RP-HPLC method below. A C18 column (250 mm×4.6 mm, i.d. 5 μm) with a column temperature of 25 °C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 μl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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Open peer comments: Debate/Discuss/Question/Opinion


DR@HAMDARD UNIVERSITY<makram\_0451@hotmail>

2010-12-16 10:08:13

I am glad to read your article Most of them are really interesting


2010-08-12 23:24:10

i appreciate your efforts in this field


2010-08-12 23:21:56

we should need to analyze the chemical active constituents of plants

Muhammad Akram@HAMDARD UNIVERSITY<makram_0451@hotmail.com>

2010-07-24 12:33:14

i want to receive your article

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