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Received: 2005-06-07

Revision Accepted: 2005-09-03

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.11 P.1087-1094


Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system

Author(s):  XU Ying, HE Guo-qing, LI Jing-jun

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Elastase, Aqueous two-phase system (ATPS), Bioseparation, Purification, Optimization, Response surface methodology (RSM)

XU Ying, HE Guo-qing, LI Jing-jun. Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system[J]. Journal of Zhejiang University Science B, 2005, 6(11): 1087-1094.

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author="XU Ying, HE Guo-qing, LI Jing-jun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system
%A XU Ying
%A HE Guo-qing
%A LI Jing-jun
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1087

T1 - Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system
A1 - XU Ying
A1 - HE Guo-qing
A1 - LI Jing-jun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 11
SP - 1087
EP - 1094
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Y1 - 2005
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B1087

This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems was investigated. The suitable system for elastase extraction was PEG/KH2PO4-K2HPO4, in which elastase is mainly partitioned into the PEG-rich phase, while the cells remained in the other phase. The influence of defined system parameters (e.g. PEG molecular mass, pH, NaCl addition) on the partitioning behavior of elastase is described. The concentration of phase forming components, PEG and KH2PO4-K2HPO4, was optimized for elastase recovery by means of response surface methodology, and it was found that they greatly influenced extraction recovery. The optimal ATPS was 23.1% (w/w) PEG 2 000 and 11.7% (w/w) KH2PO4-K2HPO4. The predicted recovery was about 89.5%, so this process is suggested to be a rapid and convenient method for elastase extraction.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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