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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.899-905


Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells

Author(s):  LI Yi, DU Bin, PAN Jia-qi, CHEN De-chang, LIU Da-wei

Affiliation(s):  Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Medical Academy, Beijing 100730, China; more

Corresponding email(s):   billliyi@yahoo.com

Key Words:  Activated protein C (APC), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Sepsis, Human umbilical vein endothelial cell (HUVEC)

LI Yi, DU Bin, PAN Jia-qi, CHEN De-chang, LIU Da-wei. Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells[J]. Journal of Zhejiang University Science B, 2006, 7(11): 899-905.

@article{title="Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells",
author="LI Yi, DU Bin, PAN Jia-qi, CHEN De-chang, LIU Da-wei",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells
%A LI Yi
%A DU Bin
%A PAN Jia-qi
%A CHEN De-chang
%A LIU Da-wei
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 899-905
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0899

T1 - Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells
A1 - LI Yi
A1 - DU Bin
A1 - PAN Jia-qi
A1 - CHEN De-chang
A1 - LIU Da-wei
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 899
EP - 905
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0899

Objective: To investigate the effect of activated protein C (APC) on inflammatory responses in human umbilical vein endothelial cells (HUVEC) stimulated with lipopolysaccharide (LPS). Methods: The second passage of collagenase digested HUVEC was divided into the following groups: serum free medium control group (SFM control), phosphate buffer solution control group (PBS control), LPS group with final concentration of 1 μg/ml (LPS group), APC group with final concentration of 7 μg/ml, Pre-APC group (APC pretreatment for 30 min prior to LPS challenge), and Post-APC group (APC administration 30 min after LPS challenge). Supernatant was harvested at 0, 4, 8, 12 and 24 h after LPS challenge. interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were analyzed with ELISA. Cells were harvested at 24 h after LPS challenge, and total RNA was extracted. Messenger RNA levels for IL-6 and IL-8 were semi-quantitatively determined by RT-PCR. Results: Compared with control group, IL-6 and IL-8 levels steadily increased 4 to 24 h after LPS stimulation. APC treatment could increase LPS-induced IL-6 and IL-8 production. The mRNA levels of IL-6 and IL-8 exhibited a similar change. Conclusion: APC can further increase the level of IL-6 and IL-8 induced by LPS. The effect of these elevated cytokines is still under investigation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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