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CLC number: R57

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Received: 2007-01-10

Revision Accepted: 2007-06-20

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.9 P.632-637

http://doi.org/10.1631/jzus.2007.B0632


Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol


Author(s):  CHEN Yan, SI Jian-min, LIU Wei-li, CAI Jian-ting, DU Qin, WANG Liang-jing, GAO Min

Affiliation(s):  Department of Gastroenterology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   sijm@163.net

Key Words:  Model, Colitis, Dextran sulfate sodium, Ethanol


CHEN Yan, SI Jian-min, LIU Wei-li, CAI Jian-ting, DU Qin, WANG Liang-jing, GAO Min. Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol[J]. Journal of Zhejiang University Science B, 2007, 8(9): 632-637.

@article{title="Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol",
author="CHEN Yan, SI Jian-min, LIU Wei-li, CAI Jian-ting, DU Qin, WANG Liang-jing, GAO Min",
journal="Journal of Zhejiang University Science B",
volume="8",
number="9",
pages="632-637",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0632"
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%0 Journal Article
%T Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol
%A CHEN Yan
%A SI Jian-min
%A LIU Wei-li
%A CAI Jian-ting
%A DU Qin
%A WANG Liang-jing
%A GAO Min
%J Journal of Zhejiang University SCIENCE B
%V 8
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%P 632-637
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0632

TY - JOUR
T1 - Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol
A1 - CHEN Yan
A1 - SI Jian-min
A1 - LIU Wei-li
A1 - CAI Jian-ting
A1 - DU Qin
A1 - WANG Liang-jing
A1 - GAO Min
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 9
SP - 632
EP - 637
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0632


Abstract: 
Several models of experimental ulcerative colitis have been reported previously. However, none of these models showed the optimum characteristics. Although dextran sulfate sodium-induced colitis results in inflammation resembling ulcerative colitis, an obvious obstacle is that dextran sulfate sodium is very expensive. The aim of this study was to develop an inexpensive model of colitis in rats. Sprague-Dawley rats were treated with 2% dextran sulfate sodium in drinking water for 3 d followed by an intracolonic administration of 30% ethanol. The administration of 2% dextran sulfate sodium followed by 30% ethanol induced significant weight loss, diarrhea and hematochezia in rats. Severe ulceration and inflammation of the distal part of rat colon were developed rapidly. Histological examination showed increased infiltration of polymorphonuclear leukocytes, lymphocytes and existence of cryptic abscesses and dysplasia. The model induced by dextran sulfate sodium at lower concentration followed by 30% ethanol is characterized by a clinical course, localization of the lesions and histopathological features similar to human ulcerative colitis and fulfills the criteria set out at the beginning of this study.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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Open peer comments: Debate/Discuss/Question/Opinion

<1>

Yan@Second Affiliated hospitalzju<chenyan72\_72@hotmail.com>

2012-10-11 00:37:53

Dear Ahmed

The DSS we used is not MW 40000 but MW 5000. Using different DSS can cause different reaction in rats.

If you have other problem please contact me .

Ahmed H. Sultan@Assisstent lecrurer of hepatology and gastroenterolgy Egypt<ahmed\_hasan\_sultan@hotmail.com>

2012-10-07 02:22:02

Dear sir

I followed your steps in conducting colitis in SD rats 170 grams using 2% DSS (MW 40.000) for 3 days followed on 4th day by 0.5 ml ethanol 30% intrarectal (about 8 cm from anus) and i had only a loose stool no blood in stool either visible or by occult blood testing 24 and 48 h after enema.

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