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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.10 P.697-703


A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion

Author(s):  YANG Xin-jian, LI Li, WU Zhong-xue

Affiliation(s):  Department of Interventional Neuroradiology, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China

Corresponding email(s):   yang-xj@163.net

Key Words:  Animal model, Collagenase, Elastase, Intracranial aneurysm

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YANG Xin-jian, LI Li, WU Zhong-xue. A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion[J]. Journal of Zhejiang University Science B, 2007, 8(10): 697-703.

@article{title="A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion",
author="YANG Xin-jian, LI Li, WU Zhong-xue",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion
%A YANG Xin-jian
%A LI Li
%A WU Zhong-xue
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 10
%P 697-703
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0697

T1 - A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion
A1 - YANG Xin-jian
A1 - LI Li
A1 - WU Zhong-xue
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 10
SP - 697
EP - 703
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0697

Background: An ideal aneurysm model of cerebral aneurysm is of great importance for studying the pathogenesis of the lesion and testing new techniques for diagnosis and treatment. Several models have been created in rabbits and are now widely used in experimental studies; however, every model has certain intrinsic limitations. Here we report the development of a novel saccular aneurysm model in rabbits using an arterial pouch that is subject to in vitro pre-digestion with combined elastase and collagenase. Methods: A segment of right common carotid artery (CCA) was dissected out and treated with elastase (60 U/ml, 20 min) followed by type I collagenase (1 mg/ml, 15 min) in vitro. The graft was anastomosed to an arterial arch built with the left CCA and the remaining right CCA, while the other end of the graft was ligated. The dimension and tissue structure of the pouch were analysed immediately, 2 or 8 weeks after operation. Findings: Ten terminal aneurysms were produced. The gross morphology of the aneurysm resembles the human cerebral terminal aneurysms. We have observed the following pathological changes: (1) growth of the aneurysm (mean diameter increased from (2.0±0.1) to (3.2±0.3) mm at 2 weeks, P<0.001, n=7~10); (2) thinning of the aneurysmal wall (the mean wall thickness decreased to 44% at 2 weeks), which was accompanied by significant losses of elastic fibres, collagen and the cellular component; and (3) spontaneous rupture (3 out of 9, one aneurysm ruptured 24 h after operation with the other two at 2 and 4 weeks respectively). Conclusion: This rabbit arterial pouch model mimics human cerebral aneurysms in relation to morphology and histology. In particular, this model exhibited an increased tendency of spontaneous rupture.

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