Full Text:   <2933>

CLC number: Q945.8

On-line Access: 

Received: 2009-06-24

Revision Accepted: 2009-09-23

Crosschecked: 2009-12-31

Cited: 16

Clicked: 5959

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.2 P.109-114


Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China

Author(s):  Hui ZHANG, Xin-ying MA, Ya-juan QIAN, Xue-ping ZHOU

Affiliation(s):  Key Laboratory of Molecular Biology of Crop Pathogens and Insects of Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   zzhou@zju.edu.cn

Key Words:  Papaya leaf curl China virus (PaLCuCNV), Tomato, Infectivity

Hui ZHANG, Xin-ying MA, Ya-juan QIAN, Xue-ping ZHOU. Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China[J]. Journal of Zhejiang University Science B, 2010, 11(2): 109-114.

@article{title="Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China",
author="Hui ZHANG, Xin-ying MA, Ya-juan QIAN, Xue-ping ZHOU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China
%A Xin-ying MA
%A Ya-juan QIAN
%A Xue-ping ZHOU
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 2
%P 109-114
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900176

T1 - Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China
A1 - Hui ZHANG
A1 - Xin-ying MA
A1 - Ya-juan QIAN
A1 - Xue-ping ZHOU
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 2
SP - 109
EP - 114
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900176

papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738–2751 nucleotides, which share 91.7%–97.9% sequence identities with PaLCuCNV isolate G2 (AJ558123). DNA-β was not found to be associated with PaLCuCNV isolates. To investigate the infectivity of PaLCuCNV, an infectious clone of PaLCuCNV-[CN:HeNZM1] was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants, which induced severe leaf curling and crinkling symptoms in these plants. Southern blot analysis and polymerase chain reaction (PCR) indicated a systemic infection of test plants by the agro-infectious clone.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Briddon, R.W., Mansoor, S., Bedford, I.D., Pinner, M.S., Saunders, K., Stanley, J., Zafar, Y., Malik, K.A., Markham, P.G., 2001. Identification of DNA components required for induction of cotton leaf curl disease. Virology, 285(2):234-243.

[2] Briddon, R.W., Bull, S.E., Mansoor, S., Amin, I., Markham, P.G., 2002. Universal primers for the PCR-mediated amplification of DNAβ: a molecule associated with some monopartite begomoviruses. Mol. Biotechnol., 20(3):315-318.

[3] Fauquet, C.M., Bisaro, D.M., Briddon, R.W., Brown, J.K., Harrison, B.D., Rybicki, E.P., Stenger, D.C., Stanley, J., 2003. Revision of taxonomic criteria for species demarcation in the family Geminiviridae, and an updated list of begomovirus species. Arch. Virol., 148(2):405-421.

[4] Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A., 2005. Geminiviridae. In: Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A. (Eds.), Virus Taxonomy 8th Report of the International Committee on Taxonomy of Viruses. Academic Press, New York, NY, USA, p.277-370.

[5] Guo, W., Yang, X.L., Xie, Y., Cui, X.F., Zhou, X.P., 2009. Tomato yellow leaf curl Thailand virus-[Y72] is a monopartite begomovirus associated with DNAβ. Virus Genes, 38(2):328-333.

[6] Harrison, B.D., Robinson, D.J., 1999. Natural genomic and antigenic variation in whitefly-transmitted geminiviruses (Begomoviruses). Annu. Rev. Phytopathol., 37(1):369-398.

[7] Li, Z.H., Zhou, X.P., Zhang, X., Xie, Y., 2004. Molecular characterization of tomato-infecting begomoviruses in Yunnan, China. Arch. Virol., 149(9):1721-1732.

[8] Moffat, A.S., 1999. Plant pathology—Geminiviruses emerge as serious crop threat. Science, 286(5446):1835.

[9] Mugiira, R.B., Liu, S.S., Zhou, X., 2008. Tomato yellow leaf curl virus and Tomato leaf curl Taiwan virus invade southeast coast of China. J. Phytopathol., 156(4):217-221.

[10] Saunders, K., Bedford, I.D., Stanley, J., 2001. Pathogenicity of a natural recombinant associated with Ageratum yellow vein disease: implications for geminivirus evolution and disease aetiology. Virology, 282(1):38-47.

[11] van Engelen, F.A., Molthoff, J.W., Conner, A.J., Nap, J.P., Pereira, A., Stiekema, W.J., 1995. pBINPLUS—an improved plant transformation vector based on pBIN19. Transgenic. Res., 4(4):288-290.

[12] Wang, X.Y., Xie, Y., Zhou, X.P., 2004. Molecular characterization of two distinct begomoviruses from papaya in China. Virus Genes, 29(3):303-309.

[13] Wu, J.B., Dai, F.M., Zhou, X.P., 2006. First report of Tomato yellow leaf curl virus in China. Plant Dis., 90(10):1359.

[14] Xie, Y., Zhou, X.P., Zhang, Z.K., Qi, Y.J., 2002. Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus. Chin. Sci. Bull., 47(3):197-200.

[15] Xie, Y., Jiang, T., Zhou, X.P., 2006. Agroinoculation shows Tobacco leaf curl Yunnan virus is a monopartite begomovirus. Eur. J. Plant Pathol., 115(4):369-375.

[16] Zhang, H., Gong, H.R., Zhou, X.P., 2009. Molecular characterization and pathogenicity of Tomato yellow leaf curl virus in China. Virus Genes, 39(2):249-255.

[17] Zhou, X.P., Xie, Y., Peng, Y., Zhang, Z.K., 2003a. Malvastrum yellow vein virus, a new Begomovirus species associated with satellite DNA molecule. Chin. Sci. Bull., 48(20):2205-2209.

[18] Zhou, X.P., Xie, Y., Tao, X.R., Zhang, Z.K., Li, Z.H., Fauquet, C.M., 2003b. Characterization of DNAβ associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A. J. Gen. Virol., 84(1):237-247.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2023 Journal of Zhejiang University-SCIENCE