CLC number: R73
On-line Access:
Received: 2009-08-29
Revision Accepted: 2009-09-22
Crosschecked: 2009-10-12
Cited: 16
Clicked: 7651
Jia-wei ZHANG, Valentina RUBIO, Shu ZHENG, Zheng-zheng SHI. Knockdown of OLA1, a regulator of oxidative stress response, inhibits motility and invasion of breast cancer cells[J]. Journal of Zhejiang University Science B, 2009, 10(11): 796-804.
@article{title="Knockdown of OLA1, a regulator of oxidative stress response, inhibits motility and invasion of breast cancer cells",
author="Jia-wei ZHANG, Valentina RUBIO, Shu ZHENG, Zheng-zheng SHI",
journal="Journal of Zhejiang University Science B",
volume="10",
number="11",
pages="796-804",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0910009"
}
%0 Journal Article
%T Knockdown of OLA1, a regulator of oxidative stress response, inhibits motility and invasion of breast cancer cells
%A Jia-wei ZHANG
%A Valentina RUBIO
%A Shu ZHENG
%A Zheng-zheng SHI
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 11
%P 796-804
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0910009
TY - JOUR
T1 - Knockdown of OLA1, a regulator of oxidative stress response, inhibits motility and invasion of breast cancer cells
A1 - Jia-wei ZHANG
A1 - Valentina RUBIO
A1 - Shu ZHENG
A1 - Zheng-zheng SHI
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 11
SP - 796
EP - 804
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0910009
Abstract: To explore the role of a novel Obg-like ATPase 1 (OLA1) in cancer metastasis, small interference RNA (siRNA) was used to knockdown the protein, and the cells were subjected to in vitro cell migration and invasion assays. Knockdown of OLA1 significantly inhibited cell migration and invasion in breast cancer cell line MDA-MB-231. The knockdown caused no changes in cell growth but affected ROS production. In wound-healing assays, decreased ROS in OLA1-knockdown cells were in situ associated with the cells’ decreased motile morphology. Further, treatment of N-acetylcysteine, a general ROS scavenger, blunted the motility and invasiveness of MDA-MB-231 cells, similar to the effect of OLA1-knockdown. These results suggest that knockdown of OLA1 inhibits breast cancer cell migration and invasion through a mechanism that involves the modulation of intracellular ROS levels.
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