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CLC number: R392.12

On-line Access: 2012-05-04

Received: 2011-08-04

Revision Accepted: 2012-01-18

Crosschecked: 2012-03-30

Cited: 18

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.5 P.386-394

http://doi.org/10.1631/jzus.B1100218


Expression and preliminary functional analysis of Siglec-F on mouse macrophages


Author(s):  Yin-he Feng, Hui Mao

Affiliation(s):  Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China

Corresponding email(s):   merrymh@126.com

Key Words:  Siglec-F, Expression, Macrophages, Phagocytosis, Eosinophil


Yin-he Feng, Hui Mao. Expression and preliminary functional analysis of Siglec-F on mouse macrophages[J]. Journal of Zhejiang University Science B, 2012, 13(5): 386-394.

@article{title="Expression and preliminary functional analysis of Siglec-F on mouse macrophages",
author="Yin-he Feng, Hui Mao",
journal="Journal of Zhejiang University Science B",
volume="13",
number="5",
pages="386-394",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100218"
}

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%T Expression and preliminary functional analysis of Siglec-F on mouse macrophages
%A Yin-he Feng
%A Hui Mao
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%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100218

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T1 - Expression and preliminary functional analysis of Siglec-F on mouse macrophages
A1 - Yin-he Feng
A1 - Hui Mao
J0 - Journal of Zhejiang University Science B
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SP - 386
EP - 394
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Y1 - 2012
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1100218


Abstract: 
Sialic acid-binding immunoglobulin-like lectin (Siglec)-F is a mouse functional paralog of human Siglec-8 that induces apoptosis in human eosinophils, and therefore may be useful as the basis of treatments for a variety of disorders associated with eosinophil hyperactivity, such as asthma. The expression pattern and functions of this protein in various cell types remain to be elucidated. The aim of this study was to determine the expression of siglec-F on mouse macrophages by immunocytochemical staining, and also to investigate the effects of siglec-F engagement by a siglec-F antibody on phagocytic activity of macrophages. The results showed that siglec-F expression was detected on mouse alveolar macrophages, but not on peritoneal macrophages. Furthermore, siglec-F engagement did not affect the phagocytic activity of alveolar macrophages in the resting state or in the activated state following stimulation by the proinflammatory mediator tumor necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS). siglec-F expression on alveolar macrophages may be a result of adaptation. macrophages actively regulate immune responses via production of cytokines. Therefore, further investigation of the effects of siglec-F engagement on immune mediators or cytokines released by alveolar macrophages is required.

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