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CLC number: S562

On-line Access: 2012-06-06

Received: 2011-10-30

Revision Accepted: 2012-02-28

Crosschecked: 2012-05-11

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.6 P.478-486


In vitro inhibition of pigmentation and fiber development in colored cotton

Author(s):  Shu-na Yuan, Waqas Malik, Shui-jin Hua, Noreen Bibi, Xue-de Wang

Affiliation(s):  College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   xdwang@zju.edu.cn

Key Words:  Cytochrome c oxidase, Polyphenol oxidase, Fiber length, Rotenone, Thiourea

Shu-na Yuan, Waqas Malik, Shui-jin Hua, Noreen Bibi, Xue-de Wang. In vitro inhibition of pigmentation and fiber development in colored cotton[J]. Journal of Zhejiang University Science B, 2012, 13(6): 478-486.

@article{title="In vitro inhibition of pigmentation and fiber development in colored cotton",
author="Shu-na Yuan, Waqas Malik, Shui-jin Hua, Noreen Bibi, Xue-de Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T In vitro inhibition of pigmentation and fiber development in colored cotton
%A Shu-na Yuan
%A Waqas Malik
%A Shui-jin Hua
%A Noreen Bibi
%A Xue-de Wang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 6
%P 478-486
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100336

T1 - In vitro inhibition of pigmentation and fiber development in colored cotton
A1 - Shu-na Yuan
A1 - Waqas Malik
A1 - Shui-jin Hua
A1 - Noreen Bibi
A1 - Xue-de Wang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 6
SP - 478
EP - 486
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100336

Colored cotton has naturally pigmented fibers. The mechanism of pigmentation in cotton fiber is not well documented. This experiment was conducted to study the effects of respiratory chain inhibitors, i.e., rotenone and thiourea, on pigmentation and fiber development in colored cotton. After 1 d post-anthesis, ovaries were harvested and developing ovules were cultured on the liquid medium containing different concentrations of rotenone and thiourea for 30 d. The results demonstrate that both respiratory inhibitors reduced fiber length and ovule development under ovule culture conditions, and the inhibition efficiency of rotenone was much higher than that of thiourea. rotenone and thiourea also showed significant effects on fiber pigment (color) development in colored cotton. In green cotton fiber, rotenone advanced fiber pigment development by 7 d at 200 μmol/L, while thiourea inhibited fiber pigmentation at all treatment levels (400, 600, 800, 1000, and 2000 μmol/L). Both respiratory inhibitors, however, had no significant effects on pigmentation of brown cotton fibers. The activities of cytochrome c oxidase (COX) and polyphenol oxidase (PPO) decreased significantly with increasing levels of both respiratory inhibitors. It is suggested that both respiratory inhibitors have important roles in deciphering the mechanism of pigmentation and fiber development in colored cotton.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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Open peer comments: Debate/Discuss/Question/Opinion


Mamoona@Cotton Research Statiom<mamoonacrs@gmail.com>

2012-05-18 01:58:30

Being a cotton breeder I found this paper very much informative and this study unvail many facts regarding the pathways involve in fibre pigmentation

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