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CLC number: Q95

On-line Access: 2013-04-03

Received: 2012-06-29

Revision Accepted: 2012-11-08

Crosschecked: 2013-03-13

Cited: 10

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.4 P.318-324


Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide

Author(s):  Yan Jiang, Jing Zhao, Hui-jing Qi, Xiao-lin Li, Shi-rong Zhang, Daniel W. Song, Chi-yang Yu, Jian-gang Gao

Affiliation(s):  Key Laboratory of the Ministry of Education for Experimental Teratology and School of Life Science, Shandong University, Jinan 250100, China; more

Corresponding email(s):   chiyangyu@hotmail.com, jggao@sdu.edu.cn

Key Words:  Premature ovarian failure, Busulfan, Cyclophosphamide, Chemotherapy, Mouse model

Yan Jiang, Jing Zhao, Hui-jing Qi, Xiao-lin Li, Shi-rong Zhang, Daniel W. Song, Chi-yang Yu, Jian-gang Gao. Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide[J]. Journal of Zhejiang University Science B, 2013, 14(4): 318-324.

@article{title="Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide",
author="Yan Jiang, Jing Zhao, Hui-jing Qi, Xiao-lin Li, Shi-rong Zhang, Daniel W. Song, Chi-yang Yu, Jian-gang Gao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide
%A Yan Jiang
%A Jing Zhao
%A Hui-jing Qi
%A Xiao-lin Li
%A Shi-rong Zhang
%A Daniel W. Song
%A Chi-yang Yu
%A Jian-gang Gao
%J Journal of Zhejiang University SCIENCE B
%V 14
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%P 318-324
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200181

T1 - Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide
A1 - Yan Jiang
A1 - Jing Zhao
A1 - Hui-jing Qi
A1 - Xiao-lin Li
A1 - Shi-rong Zhang
A1 - Daniel W. Song
A1 - Chi-yang Yu
A1 - Jian-gang Gao
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 4
SP - 318
EP - 324
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200181

busulfan/cyclophosphamide (Bu/Cy) conditioning regimen has been widely used to treat cancer patients, while their effects on major internal organs in females are not fully understood. We treated female mice with Bu/Cy, and examined the histopathology of major internal organs on Day 30 after the treatment. The results show that Bu/Cy treatment affected the ovaries most extensively, while it had less effect on the spleen, lungs, and kidneys, and no effect on the heart, liver, stomach, and pancreas. To better understand the effect of Bu/Cy on the ovaries, we counted follicles, and determined the levels of ovarian steroids. The Bu/Cy-treated mice showed a reduction of primordial and primary follicles (P<0.01) on Day 30 and a marked loss of follicles at all developmental stages (P<0.01) on Day 60. Plasma levels of estradiol and progesterone in Bu/Cy-treated mice decreased by 43.9% and 61.4%, respectively. Thus, there was a gradual process of follicle loss and low estradiol in Bu/Cy-treated mice; this is a profile similar to what is found in women with premature ovarian failure (POF). The Bu/Cy-treated mice may serve as a useful animal model to study the dynamics of follicle loss in women undergoing POF.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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