CLC number: R684.3
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2013-12-17
Cited: 5
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Ya-jun Gu, Peng Ge, Yun Mu, Jin-hai Lu, Fang Zheng, Xu-guo Sun. Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints[J]. Journal of Zhejiang University Science B, 2014, 15(1): 92-99.
@article{title="Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints",
author="Ya-jun Gu, Peng Ge, Yun Mu, Jin-hai Lu, Fang Zheng, Xu-guo Sun",
journal="Journal of Zhejiang University Science B",
volume="15",
number="1",
pages="92-99",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300046"
}
%0 Journal Article
%T Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints
%A Ya-jun Gu
%A Peng Ge
%A Yun Mu
%A Jin-hai Lu
%A Fang Zheng
%A Xu-guo Sun
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 1
%P 92-99
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300046
TY - JOUR
T1 - Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints
A1 - Ya-jun Gu
A1 - Peng Ge
A1 - Yun Mu
A1 - Jin-hai Lu
A1 - Fang Zheng
A1 - Xu-guo Sun
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 1
SP - 92
EP - 99
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300046
Abstract: Objective: Our aim was to investigate clinical and laboratory characteristics of osteoarthritic patients who had amyloid deposition in their knee joints. Methods: Synovial membranes were obtained from 36 patients with knee osteoarthritis (OA) who underwent joint replacement surgery. From this sample, the diagnosis of amyloid was determined by Congo red staining, which demonstrated apple-green birefringence under a polarized microscope. All synovial membranes were immunohistochemically characterized for the expressions of amyloid immunoglobulin light chain (AL-κ and AL-λ), serum amyloid-A (SAA), amyloidogenic transthyretin (ATTR), and amyloidogenic β2-microglobulin (Aβ2M). matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry (MALDI-TOF MS) was used to analyze transthyretin (TTR) isoforms in the serum of each patient. Results: Nine cases (25%) were found to be amyloid-positive. Immunohistochemically, eight cases (88.9%) had ATTR deposition, and one sample (11.1%) was shown to be AL-κ-positive. MALDI-TOF MS identified that the TTR in the serum of the patients was unmodified wild-type TTR, TTR-Cys-S-S-Cys, and TTR-Cys-S-S-CysGly. The age at surgery and the disease duration were significantly higher in the ATTR-positive group than in the ATTR-negative group. Knee score and function score were significantly lower in the ATTR-positive group than in the ATTR-negative group. Conclusions: amyloid deposition in synovial membranes of OA patients was found to be ATTR and AL-κ. TTR in the serum of the patients was unmodified wild-type TTR together with two isoforms. The high age at surgery, long disease duration, and a deteriorated knee function were associated with ATTR amyloid deposition in the osteoarthritic knee joints.
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