Full Text:   <2365>

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CLC number: S81

On-line Access: 2015-03-09

Received: 2014-09-24

Revision Accepted: 2015-02-08

Crosschecked: 2015-05-13

Cited: 2

Clicked: 3995

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Cun-xi Nie

http://orcid.org/0000-0003-3320-2840

Wen-ju Zhang

http://orcid.org/0000-0002-5314-9904

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.447-455

http://doi.org/10.1631/jzus.B1400255


Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens


Author(s):  Cun-xi Nie, Wen-ju Zhang, Yan-feng Liu, Wen-xia Ge, Jian-cheng Liu

Affiliation(s):  College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Corresponding email(s):   zhangwj1022@sina.com

Key Words:  Fermented cottonseed meal, Lipid metabolism, Broiler, Gene expression, Metabolomics


Cun-xi Nie,Wen-ju Zhang,Yan-feng Liu,Wen-xia Ge,Jian-cheng Liu. Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens[J]. Journal of Zhejiang University Science B, 2015, 16(6): 447-455.

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author="Cun-xi Nie,Wen-ju Zhang,Yan-feng Liu,Wen-xia Ge,Jian-cheng Liu",
journal="Journal of Zhejiang University Science B",
volume="16",
number="6",
pages="447-455",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400255"
}

%0 Journal Article
%T Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens
%A Cun-xi Nie
%A Wen-ju Zhang
%A Yan-feng Liu
%A Wen-xia Ge
%A Jian-cheng Liu
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 447-455
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400255

TY - JOUR
T1 - Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens
A1 - Cun-xi Nie
A1 - Wen-ju Zhang
A1 - Yan-feng Liu
A1 - Wen-xia Ge
A1 - Jian-cheng Liu
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 447
EP - 455
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400255


Abstract: 
This study investigated the effects of fermented cottonseed meal (FCSM) on lipid metabolites, lipid metabolism-related gene expression in liver tissues and abdominal adipose tissues, and hepatic metabolomic profiling in broiler chickens. One hundred and eighty 21-d-old broiler chickens were randomly divided into three diet groups with six replicates of 10 birds in each group. The three diets consisted of a control diet supplemented with unfermented cottonseed meal, an experimental diet of cottonseed meal fermented by Candida tropicalis, and a second experimental diet of cottonseed meal fermented by C. tropicalis plus Saccharomyces cerevisae. The results showed that FCSM intake significantly decreased the levels of abdominal fat and hepatic triglycerides (P<0.05 for both). Dietary FCSM supplementation down-regulated the mRNA expression of fatty acid synthase and acetyl CoA carboxylase in liver tissues and the lipoprotein lipase expression in abdominal fat tissues (P<0.05 for both). FCSM intake resulted in significant metabolic changes of multiple pathways in the liver involving the tricarboxylic acid cycle, synthesis of fatty acids, and the metabolism of glycerolipid and amino acids. These findings indicated that FCSM regulated lipid metabolism by increasing or decreasing the expression of the lipid-related gene and by altering multiple endogenous metabolites. lipid metabolism regulation is a complex process, this discovery provided new essential information about the effects of FCSM diets in broiler chickens and demonstrated the great potential of nutrimetabolomics in researching complex nutrients added to animal diets.

肉鸡日粮中添加发酵棉粕对其组织脂类代谢及肝脏代谢谱的影响

目的:旨在研究发酵棉粕对肉鸡脂类代谢的影响。
创新点:将荧光定量聚合酶链反应PCR和代谢组学方法相结合,就采食发酵饲料(发酵棉粕)肉鸡的组织脂类代谢相关基因及肝脏代谢谱进行分析,发现发酵棉粕具有调控肉鸡脂类代谢的作用。该研究为研究日粮中复杂营养素对动物机体代谢的调控提供了新思路。
方法:将180只21日龄肉鸡随机分为三组,每组6个重复,每个重复10只鸡。对照组饲喂未发酵棉粕底物,试验组分别饲喂热带假丝酵母发酵棉粕和热带假丝酵母与酿酒酵母复合发酵棉粕。饲喂21天后,采集肉鸡腹部脂肪和肝脏进行组织基因表达和肝脏代谢组学分析。
结论:发酵棉粕可通过下调肝脏FAS、ACC和腹脂LPL等基因的表达,改变肝脏多种内源性代谢的代谢途径,进而降低肉鸡腹部脂肪沉积和肝脏甘油三酯含量。

关键词:发酵棉粕;脂类代谢;肉鸡;基因表达;代谢组学

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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