CLC number: S881.26
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-01-13
Cited: 1
Clicked: 4548
Chen Chen, Yang-yang Fan, Xin Wang, Fei Song, Tao Jiang, Ping Qian, Shun-Ming Tang, Xing-Jia Shen. bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro[J]. Journal of Zhejiang University Science B, 2016, 17(2): 127-135.
@article{title="bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro",
author="Chen Chen, Yang-yang Fan, Xin Wang, Fei Song, Tao Jiang, Ping Qian, Shun-Ming Tang, Xing-Jia Shen",
journal="Journal of Zhejiang University Science B",
volume="17",
number="2",
pages="127-135",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500078"
}
%0 Journal Article
%T bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro
%A Chen Chen
%A Yang-yang Fan
%A Xin Wang
%A Fei Song
%A Tao Jiang
%A Ping Qian
%A Shun-Ming Tang
%A Xing-Jia Shen
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 2
%P 127-135
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500078
TY - JOUR
T1 - bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro
A1 - Chen Chen
A1 - Yang-yang Fan
A1 - Xin Wang
A1 - Fei Song
A1 - Tao Jiang
A1 - Ping Qian
A1 - Shun-Ming Tang
A1 - Xing-Jia Shen
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 2
SP - 127
EP - 135
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500078
Abstract: Based on bioinformatic analysis, we selected two novel microRNAs (miRNAs), bmo-miR-0001 and bmo-miR-0015, from high-throughput sequencing of the Bombyx mori larval posterior silk gland (PSG). Firstly, we examined the expression of bmo-miR-0001 and bmo-miR 12 different tissues of the 5th instar Day-3 larvae of the silkworm. The results showed that the expression levels of both bmo-miR-0001 and bmo-miR-0015 were obviously higher in the PSG than in other tissues, implying there is a spatio-temporal condition for bmo-miR-0001 and bmo-miR-0015 to regulate the expression of BmFib-L. To test this hypothesis, we constructed pri-bmo-miR-0001 expressing the plasmid pcDNA3.0 and pri-bmo-miR-0015 expressing the plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-0015-SV40]. Finally, the BmN cells were harvested and luciferase activity was detected. The results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected by pcDNA3.0 [ie1-egfp-pri-bmo-miR-0001-SV40] or pcDNA3.0 with pGL3.0 [A3-luc-Fib-L-3'UTR-SV40], suggesting that both bmo-miR-0001 and bmo-miR-0015 can down-regulate the expression of BmFib-L in vitro.
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