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CLC number: S188

On-line Access: 2016-06-03

Received: 2015-09-16

Revision Accepted: 2016-01-25

Crosschecked: 2016-05-12

Cited: 0

Clicked: 4061

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Sheng-rong Shen

http://orcid.org/0000-0003-4096-1693

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.465-475

http://doi.org/10.1631/jzus.B1500225


Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination


Author(s):  Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Molecularly imprinted polymer (MIP), Molecularly imprinted solid-phase extraction (MISPE), Clenbuterol, Cyclodextrin


Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen. Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination[J]. Journal of Zhejiang University Science B, 2016, 17(6): 465-475.

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author="Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen",
journal="Journal of Zhejiang University Science B",
volume="17",
number="6",
pages="465-475",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500225"
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%0 Journal Article
%T Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination
%A Sovichea Lay
%A Hai-ning Yu
%A Bao-xiang Hu
%A Sheng-rong Shen
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 6
%P 465-475
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500225

TY - JOUR
T1 - Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination
A1 - Sovichea Lay
A1 - Hai-ning Yu
A1 - Bao-xiang Hu
A1 - Sheng-rong Shen
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 465
EP - 475
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500225


Abstract: 
A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.

分子印迹聚合物作为吸附剂结合液相色谱法测定盐酸克仑特罗

目的:测定猪肝脏中微量盐酸克仑特罗的残留量。
创新点:开发一种新的瘦肉精检测方法。
方法:用β-环糊精(ally-β-CD)和甲基丙烯酸(MAA)为功能单体制作分子印迹聚合物(MIP),以盐酸克伦特罗作为模板,用乙烯乙二醇二甲基丙烯酸酯(EGDMA)作为交联剂。联合使用ally-β-CD和MAA作为二元功能单体,能合成高质量的盐酸克伦特罗模板。
结论:与丙烯腈制作的MIP(M-AN)和甲基丙烯酸甲酯制作的MIP(M-MMA)相比,MAA制作的MIP聚合物(M-MAA)被证明具有更好的选择性萃取能力,被用来进行分子印记固相萃取猪肝中残留物。研究结果发现,其回收率高(91.03%~96.76%),且相对标准偏差低(RSD≤4.45%)。因此,这种方法可以用于食品质量和安全的分析,具有简单、成本低廉的特点,能够快速检测猪肝中的瘦肉精残留量。

关键词:分子印迹聚合物;分子印迹固相萃取;盐酸克仑特罗;β-环糊精

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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