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CLC number: S188

On-line Access: 2016-06-03

Received: 2015-09-16

Revision Accepted: 2016-01-25

Crosschecked: 2016-05-12

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Sheng-rong Shen


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.465-475


Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination

Author(s):  Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Molecularly imprinted polymer (MIP), Molecularly imprinted solid-phase extraction (MISPE), Clenbuterol, Cyclodextrin

Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen. Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination[J]. Journal of Zhejiang University Science B, 2016, 17(6): 465-475.

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author="Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination
%A Sovichea Lay
%A Hai-ning Yu
%A Bao-xiang Hu
%A Sheng-rong Shen
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 6
%P 465-475
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500225

T1 - Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination
A1 - Sovichea Lay
A1 - Hai-ning Yu
A1 - Bao-xiang Hu
A1 - Sheng-rong Shen
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 465
EP - 475
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500225

A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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