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CLC number: R964

On-line Access: 2016-07-06

Received: 2015-12-18

Revision Accepted: 2016-01-21

Crosschecked: 2016-06-18

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Pan Xu

http://orcid.org/0000-0001-9324-1513

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.7 P.503-514

http://doi.org/10.1631/jzus.B1500321


Cognitive-enhancing effects of hydrolysate of polygalasaponin in SAMP8 mice


Author(s):  Pan Xu, Shu-ping Xu, Ke-zhu Wang, Cong Lu, Hong-xia Zhang, Rui-le Pan, Chang Qi, Yan-yan Yang, Ying-hui Li, Xin-min Liu

Affiliation(s):  Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; more

Corresponding email(s):   liuxinmin@hotmail.com

Key Words:  Cognitive improvement, Hydrolysate of polygalasaponin, SAMP8 mice


Pan Xu, Shu-ping Xu, Ke-zhu Wang, Cong Lu, Hong-xia Zhang, Rui-le Pan, Chang Qi, Yan-yan Yang, Ying-hui Li, Xin-min Liu. Cognitive-enhancing effects of hydrolysate of polygalasaponin in SAMP8 mice[J]. Journal of Zhejiang University Science B, 2016, 17(7): 503-514.

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author="Pan Xu, Shu-ping Xu, Ke-zhu Wang, Cong Lu, Hong-xia Zhang, Rui-le Pan, Chang Qi, Yan-yan Yang, Ying-hui Li, Xin-min Liu",
journal="Journal of Zhejiang University Science B",
volume="17",
number="7",
pages="503-514",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500321"
}

%0 Journal Article
%T Cognitive-enhancing effects of hydrolysate of polygalasaponin in SAMP8 mice
%A Pan Xu
%A Shu-ping Xu
%A Ke-zhu Wang
%A Cong Lu
%A Hong-xia Zhang
%A Rui-le Pan
%A Chang Qi
%A Yan-yan Yang
%A Ying-hui Li
%A Xin-min Liu
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 7
%P 503-514
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500321

TY - JOUR
T1 - Cognitive-enhancing effects of hydrolysate of polygalasaponin in SAMP8 mice
A1 - Pan Xu
A1 - Shu-ping Xu
A1 - Ke-zhu Wang
A1 - Cong Lu
A1 - Hong-xia Zhang
A1 - Rui-le Pan
A1 - Chang Qi
A1 - Yan-yan Yang
A1 - Ying-hui Li
A1 - Xin-min Liu
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 7
SP - 503
EP - 514
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500321


Abstract: 
Objectives: The aim of the study is to evaluate the cognitive-enhancing effects of hydrolysate of polygalasaponin (HPS) on senescence accelerate mouse P8 (SAMP8) mice, an effective Alzheimer’s disease (AD) model, and to research the relevant mechanisms. Methods: The cognitive-enhancing effects of HPS on SAMP8 mice were assessed using Morris water maze (MWM) and step-through passive avoidance tests. Then N-methyl-D-aspartate (NMDA) receptor subunit expression for both the cortex and hippocampus of mice was observed using Western blotting. Results: HPS (25 and 50 mg/kg) improved the escape rate and decreased the escape latency and time spent in the target quadrant for the SAMP8 mice in the MWM after oral administration of HPS for 10 d. Moreover, it decreased error times in the passive avoidance tests. Western blotting showed that HPS was able to reverse the levels of NMDAR1 and NMDAR2B expression in the cortex or hippocampus of model mice. Conclusions: The present study suggested that HPS can improve cognitive deficits in SAMP8 mice, and this mechanism might be associated with NMDA receptor (NMDAR)-related pathways.

远志皂苷水解产物对SAMP8小鼠的益智作用研究

目的:本文主要评价远志皂苷水解产物(HPS)对SAMP8小鼠的益智作用,并对相关机制进行探索。
创新点:HPS相较于远志皂苷有更高的安全性,毒性显著降低或消除;本研究所用的SAMP8模型是一个能较好地同时模拟阿尔兹海默病智力衰退及病理变化的代谢性模型,用于药效评价和机制研究更加可靠。
方法:采用水迷宫(图4和5)及避暗(图6)两种经典的检测方法来评价药物的益智药效,并进一步用免疫印迹实验分析N-甲基-D-天冬氨酸(NMDA)受体水平来对机制进行探索(图7)。
结论:本研究表明,HPS能明显改善SAMP8小鼠的认知损伤,其作用机制与调控NMDA受体相关通路有关。

关键词:认知改善;远志皂苷水解产物;SAMP8小鼠

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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