CLC number: Q56
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2017-05-08
Cited: 0
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Jian-zhong Xu, Wei-guo Zhang. Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance[J]. Journal of Zhejiang University Science B, 2017, 18(6): 462-473.
@article{title="Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance",
author="Jian-zhong Xu, Wei-guo Zhang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="6",
pages="462-473",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600127"
}
%0 Journal Article
%T Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance
%A Jian-zhong Xu
%A Wei-guo Zhang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 462-473
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600127
TY - JOUR
T1 - Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance
A1 - Jian-zhong Xu
A1 - Wei-guo Zhang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 462
EP - 473
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600127
Abstract: A menaquinone-7 (MK-7) high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (ARTP) mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced (7.1±0.5) mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 high-producing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved ((30.2±2.7) mg/L). In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO4 on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO4 was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached (61.3±5.2) mg/L and 2.59 mg/L per OD600 unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost.
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[32]List of electronic supplementary materials
[33]Fig. S1 Biosynthetic pathway of MK-7 and regulation mechanism by inhibition of aromatic amino acids and diphenylamine (Armougom et al., 2009)
[34]Fig. S2 Mutation rate and lethality rate of B. amyloliquefaciens Y-2 by ARTP
[35]Fig. S3 Cell growth, MK-7 production, and sugar utilization of the mutant B. amyloliquefaciens H.β.D.R.-5 after several generations
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