Full Text:   <2407>

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CLC number: R737.9

On-line Access: 2017-04-05

Received: 2016-04-24

Revision Accepted: 2016-08-17

Crosschecked: 2017-03-13

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Shi-chong Liao


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.4 P.334-342


Protein tyrosine phosphatase 1B expression contributes to the development of breast cancer

Author(s):  Shi-chong Liao, Jin-xin Li, Li Yu, Sheng-rong Sun

Affiliation(s):  Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China; more

Corresponding email(s):   sun137@sina.com

Key Words:  Protein tyrosine phosphatase 1B (PTP1B), Signal transduction and activator of transcription 3 (STAT3), Breast cancer, Tumorigenesis

Shi-chong Liao, Jin-xin Li, Li Yu, Sheng-rong Sun. Protein tyrosine phosphatase 1B expression contributes to the development of breast cancer[J]. Journal of Zhejiang University Science B, 2017, 18(4): 334-342.

@article{title="Protein tyrosine phosphatase 1B expression contributes to the development of breast cancer",
author="Shi-chong Liao, Jin-xin Li, Li Yu, Sheng-rong Sun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Protein tyrosine phosphatase 1B expression contributes to the development of breast cancer
%A Shi-chong Liao
%A Jin-xin Li
%A Li Yu
%A Sheng-rong Sun
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 4
%P 334-342
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600184

T1 - Protein tyrosine phosphatase 1B expression contributes to the development of breast cancer
A1 - Shi-chong Liao
A1 - Jin-xin Li
A1 - Li Yu
A1 - Sheng-rong Sun
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 4
SP - 334
EP - 342
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600184

The protein tyrosine phosphatase 1B (PTP1B) is an important regulator of metabolism. The relationship between PTP1B and tumors is quite complex. The purpose of this study is to explore the expression pattern and role of PTP1B in breast cancer. The expression of PTP1B was detected in 67 samples of breast cancer tissue by Western blot. Cell growth assay, Transwell migration assay, and Scratch motility assay were used to examine the proliferation and migration of MCF-7 with and without PTP1B. The total levels and phosphorylated levels of signal transduction and activator of transcription 3 (STAT3) and the expression of C-C motif chemokine ligand 5 (CCL5) were also examined by Western blot. PTP1B was overexpressed in over 70% of breast cancer tissues, correlating with patients with estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, and human epidermal growth factor receptor 2 (HER2)-positive tumors. The data also showed that both tumor size and lymph node metastasis were significantly higher in patients with a higher level of PTP1B. The proliferation and migration of MCF-7 cells were found to be inhibited after knocking down the gene of PTP1B. Our data also showed that PTP1B could up-regulate the dephosphorylated level of STAT3, which could increase the expression of CCL5. These phenomena indicated that PTP1B may play a crucial role in the development of breast cancer.


方法:收集67名乳腺癌患者的癌组织和癌旁组织标本,并记录患者临床病理学特征,使用蛋白质免疫印迹法(Western blot)检测乳腺癌组织及癌旁组织PTP1B含量;研究PTP1B水平与患者临床病理特征关系;制作PTP1B基因敲除MCF-7细胞系,分别使用细胞生长实验、Transwell细胞迁移实验和划痕活力实验检测基因敲出组与对照组细胞增殖及迁移能力,使用Western blot检测两组细胞中磷酸化与非磷酸化STAT3表达水平和CCL5表达水平。
结论:大约70%乳腺癌患者高表达PTP1B,并且随着患者TNM分期增加,患者PTP1B表达水平不断增加,同时PTP1B高表达与ER−、PR−和HER2+相关(表1和2);与对照组相比,PTP1B基因敲除组中肿瘤细胞增殖及迁移能力明显下降 (图2和3),同时PTP1B可以通过上调STAT3非磷酸化水平来提高CCL5的表达,从而加快乳腺癌发生及发展(图4)。总之,PTP1B对乳腺癌的发生起到至关重要的作用。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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