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On-line Access: 2017-06-05

Received: 2016-05-17

Revision Accepted: 2016-08-17

Crosschecked: 2017-05-08

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Citations:  Bibtex RefMan EndNote GB/T7714


An Lyu


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.481-491


Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury

Author(s):  An Lyu, Jia-jia Chen, Hui-chuan Wang, Xiao-hong Yu, Zhi-cong Zhang, Ping Gong, Lin-shu Jiang, Feng-hua Liu

Affiliation(s):  Beijing Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Corresponding email(s):   jls@bac.edu.cn, liufenghuabua@126.com

Key Words:  Bovine endometrial epithelial cell, Cytokine, Inflammatory injury, Punicalagin

An Lyu, Jia-jia Chen, Hui-chuan Wang, Xiao-hong Yu, Zhi-cong Zhang, Ping Gong, Lin-shu Jiang, Feng-hua Liu. Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury[J]. Journal of Zhejiang University Science B, 2017, 18(6): 481-491.

@article{title="Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury",
author="An Lyu, Jia-jia Chen, Hui-chuan Wang, Xiao-hong Yu, Zhi-cong Zhang, Ping Gong, Lin-shu Jiang, Feng-hua Liu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury
%A An Lyu
%A Jia-jia Chen
%A Hui-chuan Wang
%A Xiao-hong Yu
%A Zhi-cong Zhang
%A Ping Gong
%A Lin-shu Jiang
%A Feng-hua Liu
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 481-491
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600224

T1 - Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury
A1 - An Lyu
A1 - Jia-jia Chen
A1 - Hui-chuan Wang
A1 - Xiao-hong Yu
A1 - Zhi-cong Zhang
A1 - Ping Gong
A1 - Lin-shu Jiang
A1 - Feng-hua Liu
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 481
EP - 491
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600224

Objective: Bovine endometritis is one of the most common reproductive disorders in cattle. The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and to uncover the underlying mechanisms. Methods: bEECs were stimulated with different concentrations (1, 10, 30, 50, and 100 μg/ml) of LPS for 3, 6, 9, 12, and 18 h. MTT assay was used to assess cell viability and to identify the conditions for inflammatory injury and effective concentrations of punicalagin. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess gene expression of pro-inflammatory cytokines. Western blotting was used to assess levels of inflammation-related proteins. Results: Treatment of bEECs with 30 µg/ml LPS for 12 h induced cell injury and reduced cell viability. punicalagin (5, 10, or 20 µg/ml) pretreatment significantly decreased LPS-induced productions of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) in bEECs. Molecular research showed that punicalagin inhibited the activation of the upstream mediator nuclear factor-κB (NF-κB) by suppressing the production of inhibitor κBα (IκBα) and phosphorylation of p65. Results also indicated that punicalagin can suppress the phosphorylation of mitogen-activated protein kinases (MAPKs) including p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK). Conclusions: punicalagin may attenuate LPS-induced inflammatory injury and provide a potential option for the treatment of dairy cows with Escherichia coli endometritis.


方法:用不同浓度的脂多糖(1、10、30、50和100 µg/ml)刺激奶牛子宫内膜上皮细胞3、6、9、12和18 h,筛选出建立炎症损伤的最佳作用浓度和时间。安石榴苷预处理细胞2 h后用脂多糖刺激12 h,用逆转录聚合酶链式反应(RT-PCR)检测炎症因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介素8(IL-8)及肿瘤坏死因子α(TNF-α)的表达。用蛋白质免疫印迹试验(Western blotting)的方法检测核因子κB抑制蛋白α(IκBα)、磷酸化的p65、p38、c-Jun氨基末端激酶(JNK)和细胞外调节蛋白激酶(ERK)的表达水平。
结论:MTT结果显示,30 µg/ml 脂多糖刺激奶牛子宫内膜上皮细胞12 h能够造成细胞活力下降和形态改变(图2和3)。RT-PCR结果显示,安石榴苷预处理后炎症因子显著降低(图4)。Western blotting结果显示,安石榴苷预处理能显著抑制IκBα降解以及p65、p38、JNK和ERK的磷酸化表达水平(图5和6)。综上所述,安石榴苷对脂多糖诱导奶牛子宫内膜上皮细胞炎症损伤具有保护作用,在治疗奶牛子宫内膜炎中具有重要价值。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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