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CLC number: R605.971

On-line Access: 2017-01-03

Received: 2016-11-10

Revision Accepted: 2016-12-13

Crosschecked: 2016-12-16

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Yuan-qiang Lu


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.1 P.48-58


Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock

Author(s):  Jiu-kun Jiang, Wen Fang, Liang-jie Hong, Yuan-qiang Lu

Affiliation(s):  Department of Emergency Medicine, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   luyuanqiang@zju.edu.cn

Key Words:  Hemorrhagic shock, Hydroxyethyl starch, Hypertonic saline, Myeloid-derived suppressor cells, Normal saline

Jiu-kun Jiang, Wen Fang, Liang-jie Hong, Yuan-qiang Lu. Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock[J]. Journal of Zhejiang University Science B, 2017, 18(1): 48-58.

@article{title="Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock",
author="Jiu-kun Jiang, Wen Fang, Liang-jie Hong, Yuan-qiang Lu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock
%A Jiu-kun Jiang
%A Wen Fang
%A Liang-jie Hong
%A Yuan-qiang Lu
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 1
%P 48-58
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600510

T1 - Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock
A1 - Jiu-kun Jiang
A1 - Wen Fang
A1 - Liang-jie Hong
A1 - Yuan-qiang Lu
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 1
SP - 48
EP - 58
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600510

Objective: To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES). Methods: BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs). Results: The MDSCs in BMNCs among three resuscitation groups were lower 2 h after shock, in PBNCs of the HTS group were higher, and in spleen of the NS group were lower (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios in PBNCs of the HTS and HES groups were lower (both P<0.05 vs. control). At 24 h, the MDSCs in PBNCs of the NS and HTS groups were higher, while the spleen MDSCs in the HTS group were higher (all P<0.05 vs. control). The M-MDSC/ G-MDSC ratios were all less in PBNCs, spleen, and BMNCs of the NS and HTS groups, and were lower in BMNCs of the HES group (all P<0.05 vs. control). At 72 h, the elevated MDSCs in PBNCs were presented in the HTS and HES groups, and in spleen the augment turned up in three resuscitation groups (all P<0.05 vs. control). The inclined ratios to M-MDSC were exhibited in spleen of the NS and HTS groups, and in PBNCs of the NS group; the inclination to G-MDSC in BMNCs was shown in the HES group (all P<0.05 vs. control). Conclusions: HTS induces the earlier elevation of MDSCs in peripheral blood and spleen, and influences its distribution and differentiation, while HES has a less effect on the distribution but a stronger impact on the differentiation of MDSCs, especially in bone marrow.


方法:将BALB/c雄性小鼠随机分成四组,除对照组外,其余三组在建立失血性休克小鼠模型后采用不同的液体复苏:NS组、HTS组和HES组。在模型建立后的2、24和72 h分批次处死小鼠,取外周血、脾脏和骨髓细胞组织,通过三色荧光标记流式细胞术进一步分析MDSC细胞含量,以及其两亚组单核髓源性抑制细胞(M-MDSC)和中性粒髓源性抑制细胞(G-MDSC)的比值。


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