CLC number: R445.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2018-08-14
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Wei-hui Shentu, Cao-xin Yan, Chun-mei Liu, Rui-xiang Qi, Yao Wang, Zhao-xu Huang, Li-ming Zhou, Xiang-dong You. Use of cationic microbubbles targeted to P-selectin to improve ultrasound-mediated gene transfection of hVEGF165 to the ischemic myocardium[J]. Journal of Zhejiang University Science B, 2018, 19(9): 699-707.
@article{title="Use of cationic microbubbles targeted to P-selectin to improve ultrasound-mediated gene transfection of hVEGF165 to the ischemic myocardium",
author="Wei-hui Shentu, Cao-xin Yan, Chun-mei Liu, Rui-xiang Qi, Yao Wang, Zhao-xu Huang, Li-ming Zhou, Xiang-dong You",
journal="Journal of Zhejiang University Science B",
volume="19",
number="9",
pages="699-707",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700298"
}
%0 Journal Article
%T Use of cationic microbubbles targeted to P-selectin to improve ultrasound-mediated gene transfection of hVEGF165 to the ischemic myocardium
%A Wei-hui Shentu
%A Cao-xin Yan
%A Chun-mei Liu
%A Rui-xiang Qi
%A Yao Wang
%A Zhao-xu Huang
%A Li-ming Zhou
%A Xiang-dong You
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 9
%P 699-707
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700298
TY - JOUR
T1 - Use of cationic microbubbles targeted to P-selectin to improve ultrasound-mediated gene transfection of hVEGF165 to the ischemic myocardium
A1 - Wei-hui Shentu
A1 - Cao-xin Yan
A1 - Chun-mei Liu
A1 - Rui-xiang Qi
A1 - Yao Wang
A1 - Zhao-xu Huang
A1 - Li-ming Zhou
A1 - Xiang-dong You
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 9
SP - 699
EP - 707
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700298
Abstract: Gene therapies have been applied to the treatment of cardiovascular disease, but their use is limited by the need to deliver them to the right target. We have employed targeted contrast ultrasound-mediated gene transfection (TCUMGT) via ultrasound-targeted microbubble destruction (UTMD) to transfer therapeutic genes to specific anatomic and pathological targets. Phospholipid microbubbles (MBs) with pcDNA3.1-human vascular endothelial growth factor 165 (pcDNA3.1-hVEGF165) plasmids targeted to p-selectin (MB+P+VEGFp) were created by conjugating monoclonal antibodies against p-selectin to the lipid shell. These microbubbles were divided into four groups: microbubble only (MB), microbubble+p-selectin (MB+P), microbubble+pcDNA3.1-hVEGF165 plasmid (MB+VEGFp), and microbubble+ p-selectin+pcDNA3.1-hVEGF165 plasmid (MB+P+VEGFp). The reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) results showed that the VEGF gene was successfully transfected by TCUMGT and the efficiency is increased with p-selectin targeting moiety. UTMD-mediated delivery of VEGF increased myocardial vascular density and improved cardiac function, and MB+P+VEGFp delivery showed greater improvement than MB+VEGFp. This study drew support from TCUGMT technology and took advantage of targeted ultrasound contrast agent to identify ischemic myocardium, release pcDNA3.1-hVEGF165 recombinant plasmid, and improve the myocardial microenvironment, so promoting the restoration of myocardial function.
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