Full Text:   <2804>

Summary:  <1803>

Suppl. Mater.: 

CLC number: S335.3

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2018-02-10

Cited: 0

Clicked: 5164

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Min-tao Zhong

https://orcid.org/0000-0002-2190-1153

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.4 P.263-273

http://doi.org/10.1631/jzus.B1700337


PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice


Author(s):  Jing Ye, Yao-Long Yang, Xing-Hua Wei, Xiao-Jun Niu, Shan Wang, Qun Xu, Xiao-Ping Yuan, Han-Yong Yu, Yi-Ping Wang, Yue Feng, Shu Wang

Affiliation(s):  College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; more

Corresponding email(s):   fy_555500@163.com, wangshusl@126.com

Key Words:  Pale-green leaf, Chlorophyll synthesis, Reactive oxygen species, Senescence, Rice


Jing Ye, Yao-Long Yang, Xing-Hua Wei, Xiao-Jun Niu, Shan Wang, Qun Xu, Xiao-Ping Yuan, Han-Yong Yu, Yi-Ping Wang, Yue Feng, Shu Wang. PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice[J]. Journal of Zhejiang University Science B, 2018, 19(4): 263-273.

@article{title="PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice",
author="Jing Ye, Yao-Long Yang, Xing-Hua Wei, Xiao-Jun Niu, Shan Wang, Qun Xu, Xiao-Ping Yuan, Han-Yong Yu, Yi-Ping Wang, Yue Feng, Shu Wang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="4",
pages="263-273",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700337"
}

%0 Journal Article
%T PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice
%A Jing Ye
%A Yao-Long Yang
%A Xing-Hua Wei
%A Xiao-Jun Niu
%A Shan Wang
%A Qun Xu
%A Xiao-Ping Yuan
%A Han-Yong Yu
%A Yi-Ping Wang
%A Yue Feng
%A Shu Wang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 4
%P 263-273
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700337

TY - JOUR
T1 - PGL3 is required for chlorophyll synthesis and impacts leaf senescence in rice
A1 - Jing Ye
A1 - Yao-Long Yang
A1 - Xing-Hua Wei
A1 - Xiao-Jun Niu
A1 - Shan Wang
A1 - Qun Xu
A1 - Xiao-Ping Yuan
A1 - Han-Yong Yu
A1 - Yi-Ping Wang
A1 - Yue Feng
A1 - Shu Wang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 4
SP - 263
EP - 273
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700337


Abstract: 
rice leaf color mutants play a great role in research about the formation and development of chloroplasts and the genetic mechanism of the chlorophyll (Chl) metabolism pathway. pgl3 is a rice leaf color mutant derived from Xiushui11 (Oryza sativa L. spp. japonica), treated with ethyl methane sulfonate (EMS). The mutant exhibited a pale-green leaf (pgl) phenotype throughout the whole development as well as reduced grain quality. Map-based cloning of PGL3 revealed that it encodes the chloroplast signal recognition particle 43 kDa protein (cpSRP43). PGL3 affected the Chl synthesis by regulating the expression levels of the Chl synthesis-associated genes. Considerable reactive oxygen species were accumulated in the leaves of pgl3, and the transcription levels of its scavenging genes were down-regulated, indicating that pgl3 can accelerate senescence. In addition, high temperatures could inhibit the plant’s growth and facilitate the process of senescence in pgl3.

PGL3对水稻叶绿素合成和叶片衰老的影响

目的:研究PGL3的遗传机制与生物学功能.
创新点:研究一个水稻叶色突变体的鉴定与基因克隆,并探讨其对叶绿素合成和叶片衰老的影响.
方法:通过乙基甲磺酸(EMS)诱变,获得了一个淡绿叶突变体pgl3,运用图位克隆法对PGL3进行定位,并对PGL3的功能进行研究.
结论:PGL3通过调节叶绿素合成相关基因的表达水平影响叶绿素合成.pgl3叶片中清除活性氧基因转录水平下调,导致活性氧大量积累,表明pgl3可以加速衰老.此外,高温可以抑制植物的生长并加速pgl3衰老.

关键词:淡绿叶;叶绿素合成;活性氧;衰老;水稻

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Austin JR, Frost E, Vidi PA, et al., 2006. Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes. Plant Cell, 18(7):1693-1703.

[2]Bréhélin C, Kessler F, van Wijk KJ, 2007. Plastoglobules: versatile lipoprotein particles in plastids. Trends Plant Sci, 12(6):260-266.

[3]Carol P, Stevenson D, Bisanz C, et al., 1999. Mutations in the Arabidopsis gene IMMUTANS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation. Plant Cell, 11(1): 57-68.

[4]Davletova S, Rizhsky L, Liang HJ, et al., 2005. Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell, 17(1):268-281.

[5]Deng XJ, Zhang HQ, Wang Y, et al., 2014. Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp. indica). PLoS ONE, 9(6):e99564.

[6]Fukao T, Yeung E, Bailey-Serres J, 2011. The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice. Plant Cell, 23(1):412-427.

[7]Goh CH, Jung KH, Roberts SK, et al., 2004. Mitochondria provide the main source of cytosolic ATP for activation of outward-rectifying K+ channels in mesophyll protoplast of chlorophyll-deficient mutant rice (OsCHLH) seedlings. J Biol Chem, 279(8):6874-6882.

[8]Groves MR, Mant A, Kuhn A, et al., 2001. Functional characterization of recombinant chloroplast signal recognition particle. J Biol Chem, 276(30):27778-27786.

[9]Huang JL, Qin F, Zang GC, et al., 2013. Mutation of OsDET1 increases chlorophyll content in rice. Plant Sci, 210: 241-249.

[10]Huang QN, Shi YF, Zhang XB, et al., 2016. Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice. J Integr Plant Biol, 58(1):12-28.

[11]Hutin C, Havaux M, Carde JP, et al., 2002. Double mutation cpSRP43/cpSRP54 is necessary to abolish the cpSRP pathway required for thylakoid targeting of the light-harvesting chlorophyll proteins. Plant J, 29(5):531-543.

[12]Klimyuk VI, Persello-Cartieaux F, Havaux M, et al., 1999. A chromodomain protein encoded by the Arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Plant Cell, 11(1):87-99.

[13]Kusaba M, Ito H, Morita R, et al., 2007. Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence. Plant Cell, 19(4):1362-1375.

[14]Lee S, Park C, 2012. Regulation of reactive oxygen species generation under drought conditions in Arabidopsis. Plant Signal Behav, 7(6):599-601.

[15]Lee S, Kim JH, Yoo ES, et al., 2005. Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 57(6):805-818.

[16]Li W, Zhong SH, Li GJ, et al., 2011. Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence. Cell Res, 21(5):835-848.

[17]Li XM, Chao DY, Wu Y, et al., 2015. Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice. Nat Genet, 47(7): 827-833.

[18]Liang CZ, Zheng GY, Li WZ, et al., 2015. Melatonin delays leaf senescence and enhances salt stress tolerance in rice. J Pineal Res, 59(1):91-101.

[19]Liu WZ, Fu YP, Hu GC, et al., 2007. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta, 226(3):785-795.

[20]Liu XQ, Bai XQ, Wang XJ, et al., 2007. OsWRKY71, a rice transcription factor, is involved in rice defense response. J Plant Physiol, 164(8):969-979.

[21]Long SP, Zhu XG, Naidu SL, et al., 2006. Can improvement in photosynthesis increase crop yields? Plant Cell Environ, 29(3):315-330.

[22]Lv XG, Shi YF, Xu X, et al., 2015. Oryza sativa chloroplast signal recognition particle 43 (OscpSRP43) is required for chloroplast development and photosynthesis. PLoS ONE, 10(11):e0143249.

[23]Mhamdi A, Queval G, Chaouch S, et al., 2010. Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. J Exp Bot, 61(15):4197-4220.

[24]Mittler R, Vanderauwera S, Gollery M, et al., 2004. Reactive oxygen gene network of plants. Trends Plant Sci, 9(10): 490-498.

[25]Nagata N, Tanaka R, Satoh S, et al., 2005. Identification of a vinyl reductase gene for chlorophyll synthesis in Arabidopsis thaliana and implications for the evolution of Prochlorococcus species. Plant Cell, 17(1):233-240.

[26]Park SY, Yu JW, Park JS, et al., 2007. The senescence-induced staygreen protein regulates chlorophyll degradation. Plant Cell, 19(5):1649-1664.

[27]Piquery L, Davoine C, Huault C, et al., 2000. Senescence of leaf sheaths of ryegrass stubble: changes in enzyme activities related to H2O2 metabolism. Plant Growth Regul, 30(1):71-77.

[28]Ramundo S, Casero D, Mühlhaus T, et al., 2014. Conditional depletion of the Chlamydomonas chloroplast ClpP protease activates nuclear genes involved in autophagy and plastid protein quality control. Plant Cell, 26(5):2201-2222.

[29]Richards RA, 2000. Selectable traits to increase crop photosynthesis and yield of grain crops. J Exp Bot, 51(Suppl 1):447-458.

[30]Sakuraba Y, Rahman ML, Cho SH, et al., 2013. The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J, 74(1):122-133.

[31]Sato Y, Morita R, Katsuma S, et al., 2009. Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice. Plant J, 57(1):120-131.

[32]Sato Y, Masuta Y, Saito K, et al., 2011. Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa. Plant Cell Rep, 30(3):399-406.

[33]Wang P, Gao J, Wan C, et al., 2010. Divinyl chlorophyll (ide) a can be converted to monovinyl chlorophyll (ide) a by a divinyl reductase in rice. Plant Physiol, 153(3): 994-1003.

[34]Wang P, Wan C, Xu Z, et al., 2013. One divinyl reductase reduces the 8-vinyl groups in various intermediates of chlorophyll biosynthesis in a given higher plant species, but the isozyme differs between species. Plant Physiol, 161(1):521-534.

[35]Wang YH, Zhang LR, Zhang LL, et al., 2013. A novel stress-associated protein SbSAP14 from Sorghum bicolor confers tolerance to salt stress in transgenic rice. Mol Breed, 32(2):437-449.

[36]Wang ZW, Zhang TQ, Xing YD, et al., 2016. YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development. J Integr Agric, 15(5):944-953.

[37]Wellburn AR, 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol, 144(3):307-313.

[38]Wu XY, Kuai BK, Jia JZ, et al., 2012. Regulation of leaf senescence and crop genetic improvement. J Integr Plant Biol, 54(12):936-952.

[39]Yang YL, Xu J, Huang LC, et al., 2016. PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice. J Exp Bot, 67(5):1297-1310.

[40]Zhang F, Luo X, Hu B, et al., 2013. YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice. Rice, 6(1):7.

[41]Zhang H, Li J, Yoo JH., et al., 2006. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 62(3):325-337.

[42]Zhou L, Liang S, Ponce K, et al., 2015. Factors affecting head rice yield and chalkiness in indica rice. Field Crops Res, 172:1-10.

[43]Zhu YY, Nomura T, Xu YH, et al., 2006. ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell, 18(2):442-456.

[44]List of electronic supplementary materials

[45]Table S1 Molecular markers used for mapping of the mutation

[46]Table S2 List of genes used for real-time PCR analysis

[47]Fig. S1 Rice quality traits in WT and pgl3

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE