CLC number: Q812
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2018-08-07
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Yan-Fang Zhao, dan-Dan Lu, Andreas Bechthold, Zheng Ma, Xiao-Ping Yu. Impact of otrA expression on morphological differentiation, actinorhodin production, and resistance to aminoglycosides in Streptomyces coelicolor M145[J]. Journal of Zhejiang University Science B, 2018, 19(9): 708-717.
@article{title="Impact of otrA expression on morphological differentiation, actinorhodin production, and resistance to aminoglycosides in Streptomyces coelicolor M145",
author="Yan-Fang Zhao, dan-Dan Lu, Andreas Bechthold, Zheng Ma, Xiao-Ping Yu",
journal="Journal of Zhejiang University Science B",
volume="19",
number="9",
pages="708-717",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800046"
}
%0 Journal Article
%T Impact of otrA expression on morphological differentiation, actinorhodin production, and resistance to aminoglycosides in Streptomyces coelicolor M145
%A Yan-Fang Zhao
%A dan-Dan Lu
%A Andreas Bechthold
%A Zheng Ma
%A Xiao-Ping Yu
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 9
%P 708-717
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800046
TY - JOUR
T1 - Impact of otrA expression on morphological differentiation, actinorhodin production, and resistance to aminoglycosides in Streptomyces coelicolor M145
A1 - Yan-Fang Zhao
A1 - dan-Dan Lu
A1 - Andreas Bechthold
A1 - Zheng Ma
A1 - Xiao-Ping Yu
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 9
SP - 708
EP - 717
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800046
Abstract: otrA resembles elongation factor G (EF-G) and is considered to be an oxytetracycline (OTC)-resistance determinant in Streptomyces rimosus. In order to determine whether otrA also conferred resistance to OTC and other aminoglycosides to Streptomyces coelicolor, the otrA gene from S. rimosus M527 was cloned under the control of the strong ermE* promoter. The resulting plasmid, pIB139-otrA, was introduced into S. coelicolor M145 by intergeneric conjugation, yielding the recombinant strain S. coelicolor M145-OA. As expected S. coelicolor M145-OA exhibited higher resistance levels specifically to OTC and aminoglycosides gentamycin, hygromycin, streptomycin, and spectinomycin. However, unexpectedly, S. coelicolor M145-OA on solid medium showed an accelerated aerial mycelia formation, a precocious sporulation, and an enhanced actinorhodin (Act) production. Upon growth in 5-L fermentor, the amount of intra- and extracellular Act production was 6-fold and 2-fold higher, respectively, than that of the original strain. Consistently, reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcriptional level of pathway-specific regulatory gene actII-orf4 was significantly enhanced in S. coelicolor M145-OA compared with in S. coelicolor M145.
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[37]List of electronic supplementary materials
[38]Fig. S1 Phenotypic verification of seven randomly recombinant strains of S. coelicolor M145-OA
[39]Fig. S2 PCR analysis of otrA gene from S. coelicolor M145-OA
[40]Fig. S3 Morphological analyses of S. rimosus M527 and S. rimosus M527-OA on MS medium
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