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On-line Access: 2019-04-01

Received: 2018-11-06

Revision Accepted: 2019-01-29

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.4 P.343-354


Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus

Author(s):  De-Qing Huang, Rui Chen, Ya-Qin Wang, Jian Hong, Xue-Ping Zhou, Jian-Xiang Wu

Affiliation(s):  State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   zzhou@zju.edu.cn, wujx@zju.edu.cn

Key Words:  Monoclonal antibody, Colloidal gold-based immunochromatographic strip, Rice stripe virus, Rice, Small brown planthopper

De-Qing Huang, Rui Chen, Ya-Qin Wang, Jian Hong, Xue-Ping Zhou, Jian-Xiang Wu. Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus[J]. Journal of Zhejiang University Science B, 2019, 20(4): 343-354.

@article{title="Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus",
author="De-Qing Huang, Rui Chen, Ya-Qin Wang, Jian Hong, Xue-Ping Zhou, Jian-Xiang Wu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus
%A De-Qing Huang
%A Rui Chen
%A Ya-Qin Wang
%A Jian Hong
%A Xue-Ping Zhou
%A Jian-Xiang Wu
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 4
%P 343-354
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800563

T1 - Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus
A1 - De-Qing Huang
A1 - Rui Chen
A1 - Ya-Qin Wang
A1 - Jian Hong
A1 - Xue-Ping Zhou
A1 - Jian-Xiang Wu
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 4
SP - 343
EP - 354
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800563

rice stripe virus%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>rice stripe virus (RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies (MAbs) 16E6 and 11C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper (SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and non-viruliferous SBPH. Sensitivity analyses showed that the test strip could detect the virus in RSV-infected rice plant tissue crude extracts diluted to 1:20 480 (w/v, g/mL), and in individual viruliferous SBPH homogenate diluted to 1:2560 (individual SPBH/μL). The validity of the developed strip was further confirmed by tests using field-collected rice and SBPH samples. This newly developed test strip is a low-cost, fast, and easy-to-use tool for on-site detection of RSV infection during field epidemiological studies and paddy field surveys, and thus can benefit decision-making for RSV management in the field.


创新点:利用制备的RSV单克隆抗体,首次制备了能在5~10 min内快速、准确、灵敏、特异地检测水稻植物和灰飞虱体内RSV的胶体金免疫层析试纸条.
结论:利用杂交瘤技术获得了2株RSV单克隆抗体(16E6和11C1),胶体金标记16E6单克隆抗体包被在聚酯膜制成的结合垫上,以11C1单克隆抗体和羊抗鼠抗体分别包被到硝酸纤维素膜的检测线和质控线,制成能在5~10 min内快速、特异、灵敏、准确地检测田间水稻及单头灰飞虱样品中RSV的胶体金免疫层析试纸条.试纸条检测感染RSV的水稻植株和灰飞虱呈阳性反应,而检测健康水稻和感染其它5种常见水稻病毒的植株及其传毒介体呈阴性反应,且试纸条检测RSV感染水稻植物组织的灵敏度达到1:20 480倍稀释(w/v, g/mL),检测携带RSV单头灰飞虱的灵敏度达到1:2560倍稀释(单头灰飞虱/µL).田间样品检测结果发现,试纸条检测结果与反转录聚合酶链反应(RT-PCR)的检测结果一致,表明制备的试纸条可有效地用于田间RSV的检测,从而为水稻条纹叶枯病的诊断、监测预警、抗病育种、流行病学研究及科学防治提供技术和物质支撑.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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