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Received: 2019-02-20

Revision Accepted: 2019-06-30

Crosschecked: 2019-08-08

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Jian-Ping duan


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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.10 P.793-802


Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals

Author(s):  Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan

Affiliation(s):  Department of Infectious Disease, Qingdao Sixth Peoples Hospital, Qingdao 266033, China

Corresponding email(s):   duanjianping20@163.com

Key Words:  Acquired immune deficiency syndrome (AIDS), Tumor necrosis factor-α, (TNF-α, ), Interleukin-6 (IL-6), Intestinal flora, CD4+ T lymphocytes

Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan. Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals[J]. Journal of Zhejiang University Science B, 2019, 20(10): 793-802.

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author="Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals
%A Jing Lu
%A Sai-Sai Ma
%A Wei-Ying Zhang
%A Jian-Ping Duan
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 10
%P 793-802
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900075

T1 - Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals
A1 - Jing Lu
A1 - Sai-Sai Ma
A1 - Wei-Ying Zhang
A1 - Jian-Ping Duan
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 10
SP - 793
EP - 802
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900075

Objective: In this study, we investigated the changes in peripheral blood inflammatory factors and intestinal flora in )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>acquired immune deficiency syndrome (AIDS) and human immunodeficiency virus (HIV)-positive individuals (AIDS/HIV patients), and explored the relationships among intestinal flora, peripheral blood inflammatory factors, and CD4+ T lymphocytes. Methods: Thirty blood and stool samples from an AIDS group and a control group were collected. The levels of tumor necrosis factor-α; (TNF-α;) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA), and the number of CD4+ T lymphocytes by a FACSCount automated instrument. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the messenger RNA (mRNA) levels of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis, and Enterococcus faecium. Correlations among intestinal flora, inflammatory factor levels, and CD4+ T lymphocyte values were evaluated using the Spearman correlation coefficient. Results: The levels of TNF-α and IL-6 in the AIDS group were higher than those in the control group, while the number of CD4+ T lymphocytes was lower. The amounts of Bifidobacterium and Lactobacillus in the AIDS group were significantly lower than those in control group, while the amounts of E. coli, E. faecalis, and E. faecium were much higher. The amounts of Bifidobacterium and Lactobacillus were negatively correlated with the content of TNF-α and IL-6 and the CD4+ T lymphocyte count, while those correlations were reversed for E. coli, E. faecalis, and E. faecium. Conclusions: The intestinal microbiota of AIDS/HIV patients were disordered, and there was a correlation between the amount of intestinal flora and the number of CD4+ T lymphocytes and the levels of TNF-α and IL-6.


目的:本研究探究获得性免疫缺陷综合征(AIDS)和人类免疫缺陷病毒(HIV)阳性个体患者外周血炎症因子和肠道菌群的变化,同时探讨了肠道菌群、外周血炎症因子和CD4+ T淋巴细胞之间的关系.
创新点:首次探讨了AIDS和HIV阳性个体患者中肠道菌群、外周血炎症因子和CD4+ T淋巴细胞之间的关系.
方法:从艾滋病组和对照组分别收集30份血液和粪便样本.使用酶联免疫吸附测定(ELISA)法测定肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)的水平;用流式细胞仪测定CD4+ T淋巴细胞数目;采用实时定量聚合酶链式反应(qRT-PCR)法检测双歧杆菌、乳酸菌、大肠杆菌、粪肠球菌和屎肠球菌的mRNA水平;采用Spearman分析肠道菌群、炎症因子与CD4+ T淋巴细胞之间的相关性.
结论:不但AIDS/HIV患者体内肠道菌群紊乱,而且肠道菌群数量与CD4+ T淋巴细胞数量以及TNF-α、IL-6水平存在相关性.

关键词:获得性免疫缺陷综合征;肿瘤坏死因子(TNF-α);白细胞介素-6(IL-6);肠道菌群;CD4+ T淋巴细胞

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Bäckhed F, Fraser CM, Ringel Y, et al., 2012. Defining a healthy human gut microbiome: current concepts, future directions, and clinical applications. Cell Host Microbe, 12(5):611-622.

[2]Brenchley JM, 2013. Mucosal immunity in human and simian immunodeficiency lentivirus infections. Mucosal Immunol, 6(4):657-665.

[3]Capeau J, 2011. Premature aging and premature age-related comorbidities in HIV-infected patients: facts and hypotheses. Clin Infect Dis, 53(11):1127-1129.

[4]de Medeiros RM, Valverde-Villegas JM, Junqueira DM, et al., 2016. Rapid and slow progressors show increased IL-6 and IL-10 levels in the pre-AIDS stage of HIV infection. PLoS ONE, 11(5):e0156163.

[5]de Pablo-Bernal RS, Ruiz-Mateos E, Rosado I, et al., 2014. TNF-α levels in HIV-infected patients after long-term suppressive cART persist as high as in elderly, HIV-uninfected subjects. J Antimicrob Chemother, 69(11):3041-3046.

[6]Dillon SM, Lee EJ, Kotter CV, et al., 2014. An altered intestinal mucosal microbiome in HIV-1 infection is associated with mucosal and systemic immune activation and endotoxemia. Mucosal Immunol, 7(4):983-994.

[7]Dinh DM, Volpe GE, Duffalo C, et al., 2015. Intestinal microbiota, microbial translocation, and systemic inflammation in chronic HIV infection. J Infect Dis, 211(1):19-27.

[8]Fragoso JM, Vargas Alarcón G, Jiménez Morales S, et al., 2014. Tumor necrosis factor alpha (TNF-α) in autoimmune diseases (AIDs):molecular biology and genetics. Gac Méd Méx, 150(4):334-344 (in Spanish).

[9]Gedle D, Kumera G, Eshete T, et al., 2017. Intestinal parasitic infections and its association with undernutrition and CD4 T cell levels among HIV/AIDs patients on HAART in Butajira, Ethiopia. J Health Popul Nutr, 36:15.

[10]He YW, Cai WP, Cen YW, et al., 2014. A preliminary study on intestinal microflora of AIDS patients and its correlation with CD4+ T lymphocytes. Guangdong Med J, 35(14):2172-2174 (in Chinese).

[11]Hirano T, Kishimoto T, 1989. Interleukin-6: possible implications in human diseases. Res Clin Lab, 19(1):1-10.

[12]Irvine SL, Hummelen R, Hekmat S, et al., 2010. Probiotic yogurt consumption is associated with an increase of CD4 count among people living with HIV/AIDs. J Clin Gastroenterol, 44(9):e201-e205.

[13]Klase Z, Ortiz A, Deleage C, et al., 2015. Dysbiotic bacteria translocate in progressive SIV infection. Mucosal Immunol, 8(5):1009-1020.

[14]Klatt NR, Villinger F, Bostik P, et al., 2008. Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys. J Clin Invest, 118(6):2039-2049.

[15]Kolgiri V, Patil VW, 2017. Protein carbonyl content: a novel biomarker for aging in HIV/AIDs patients. Braz J Infect Dis, 21(1):35-41.

[16]Lei Y, Wang KH, Gong KM, et al., 2012. Real-time fluorescence quantitative PCR analysis of intestinal flora in patients with acquired immune deficiency syndrome. J Pract Med, 28(1):69-71 (in Chinese).

[17]Li L, Zhong Q, 2017. Correlation of intestinal microflora with cytokines and Toll-like receptors expression in patients with ulcerative colitis. Infect Dis Inf, 30(6):361-364 (in Chinese).

[18]Lu W, Feng YQ, Jing FH, et al., 2018. Association between gut microbiota and CD4 recovery in HIV-1 infected patients. Front Microbiol, 9:1451.

[19]Lyte M, 2013. Microbial endocrinology in the microbiome-gut-brain axis: how bacterial production and utilization of neurochemicals influence behavior. PLoS Pathog, 9(11):e1003726.

[20]Maartens G, Celum C, Lewin SR, 2014. HIV infection: epidemiology, pathogenesis, treatment, and prevention. Lancet, 384(9939):258-271.

[21]Márquez M, del Álamo CFG, Girón-González JA, 2016. Gut epithelial barrier dysfunction in human immunodeficiency virus-hepatitis C virus coinfected patients: influence on innate and acquired immunity. World J Gastroenterol, 22(4):1433-1448.

[22]Monachese M, Cunningham-Rundles S, Diaz MA, et al., 2011. Probiotics and prebiotics to combat enteric infections and HIV in the developing world: a consensus report. Gut Microbes, 2(3):198-207.

[23]Monaco CL, Gootenberg DB, Zhao GY, et al., 2016. Altered virome and bacterial microbiome in human immunodeficiency virus-associated acquired immunodeficiency syndrome. Cell Host Microbe, 19(3):311-322.

[24]O'Shea JJ, Paul WE, 2010. Mechanisms underlying lineage commitment and plasticity of helper CD4+ T cells. Science, 327(5969):1098-1102.

[25]Ponte R, Mehraj V, Ghali P, et al., 2016. Reversing gut damage in HIV infection: using non-human primate models to instruct clinical research. EBioMedicine, 4:40-49.

[26]Quiloan MLG, Vu J, Carvalho J, 2012. Enterococcus faecalis can be distinguished from Enterococcus faecium via differential susceptibility to antibiotics and growth and fermentation characteristics on mannitol salt agar. Front Biol, 7(2):167-177.

[27]Ren FN, 2018. Analysis on the influence of traditional Chinese medicine comprehensive intervention on AIDS/HIV patients. Guangming J Chin Med, 33(11):1585-1587 (in Chinese).

[28]Rosado-Sánchez I, Jarrín I, Pozo-Balado MM, et al., 2017. Higher levels of IL-6, CD4 turnover and Treg frequency are already present before cART in HIV-infected subjects with later low CD4 recovery. Antiviral Res, 142:76-82.

[29]Routy B, le Chatelier E, Derosa L, et al., 2018. Gut microbiome influences efficacy of PD-1-based immunotherapy against epithelial tumors. Science, 359(6371):91-97.

[30]Shah S, Kongre V, Kumar V, et al., 2016. A study of parasitic and bacterial pathogens associated with diarrhea in HIV-positive patients. Cureus, 8(9):e807.

[31]Sobti RC, Berhane N, Mahedi SA, et al., 2010. Polymorphisms of IL-6 174 G/C, IL-10-592 C/A and risk of HIV/AIDS among North Indian population. Mol Cell Biochem, 337(1-2):145-152.

[32]Taiwo BO, Li X, Palella F, et al., 2009. Higher risk of AIDS or death in patients with lower CD4 cell counts after virally suppressive HAART. HIV Med, 10(10):657-660.

[33]Utay NS, Hunt PW, 2016. Role of immune activation in progression to AIDS. Curr Opin HIV AIDS, 11(2):131-137.

[34]Vujkovic-Cvijin I, Dunham RM, Iwai S, et al., 2013. Dysbiosis of the gut microbiota is associated with HIV disease progression and tryptophan catabolism. Sci Transl Med, 5(193):193ra91.

[35]Wang ZQ, Orlikowsky T, Dudhane A, et al., 1994. Deletion of T lymphocytes in human CD4 transgenic mice induced by HIV-gp120 and gp120-specific antibodies from AIDS patients. Eur J Immunol, 24(7):1553-1557.

[36]Yao F, Lu YQ, Jiang JK, et al., 2017. Immune recovery after fluid resuscitation in rats with severe hemorrhagic shock. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 18(5):402-409.

[37]Zhang J, Liu H, Wei B, 2017. Immune response of T cells during herpes simplex virus type 1 (HSV-1) infection. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 18(4):277-288.

[38]Zhang SG, Song YX, Shu XM, et al., 2017. A simple method for removing low-density granulocytes to purify T lymphocytes from peripheral blood mononuclear cells. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 18(7):605-614.

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