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On-line Access: 2019-09-06

Received: 2019-02-20

Revision Accepted: 2019-06-30

Crosschecked: 2019-08-08

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Jian-Ping duan


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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.10 P.793-802


Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals

Author(s):  Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan

Affiliation(s):  Department of Infectious Disease, Qingdao Sixth People’s Hospital, Qingdao 266033, China

Corresponding email(s):   duanjianping20@163.com

Key Words:  Acquired immune deficiency syndrome (AIDS), Tumor necrosis factor-α, (TNF-α, ), Interleukin-6 (IL-6), Intestinal flora, CD4+ T lymphocytes

Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan. Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals[J]. Journal of Zhejiang University Science B, 2019, 20(10): 793-802.

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author="Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals
%A Jing Lu
%A Sai-Sai Ma
%A Wei-Ying Zhang
%A Jian-Ping Duan
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 10
%P 793-802
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900075

T1 - Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals
A1 - Jing Lu
A1 - Sai-Sai Ma
A1 - Wei-Ying Zhang
A1 - Jian-Ping Duan
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 10
SP - 793
EP - 802
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900075

Objective: In this study, we investigated the changes in peripheral blood inflammatory factors and intestinal flora in )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>acquired immune deficiency syndrome (AIDS) and human immunodeficiency virus (HIV)-positive individuals (AIDS/HIV patients), and explored the relationships among intestinal flora, peripheral blood inflammatory factors, and CD4+ T lymphocytes. Methods: Thirty blood and stool samples from an AIDS group and a control group were collected. The levels of tumor necrosis factor-α; (TNF-α;) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA), and the number of CD4+ T lymphocytes by a FACSCount automated instrument. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the messenger RNA (mRNA) levels of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis, and Enterococcus faecium. Correlations among intestinal flora, inflammatory factor levels, and CD4+ T lymphocyte values were evaluated using the Spearman correlation coefficient. Results: The levels of TNF-α and IL-6 in the AIDS group were higher than those in the control group, while the number of CD4+ T lymphocytes was lower. The amounts of Bifidobacterium and Lactobacillus in the AIDS group were significantly lower than those in control group, while the amounts of E. coli, E. faecalis, and E. faecium were much higher. The amounts of Bifidobacterium and Lactobacillus were negatively correlated with the content of TNF-α and IL-6 and the CD4+ T lymphocyte count, while those correlations were reversed for E. coli, E. faecalis, and E. faecium. Conclusions: The intestinal microbiota of AIDS/HIV patients were disordered, and there was a correlation between the amount of intestinal flora and the number of CD4+ T lymphocytes and the levels of TNF-α and IL-6.


目的:本研究探究获得性免疫缺陷综合征(AIDS)和人类免疫缺陷病毒(HIV)阳性个体患者外周血炎症因子和肠道菌群的变化,同时探讨了肠道菌群、外周血炎症因子和CD4+ T淋巴细胞之间的关系.
创新点:首次探讨了AIDS和HIV阳性个体患者中肠道菌群、外周血炎症因子和CD4+ T淋巴细胞之间的关系.
方法:从艾滋病组和对照组分别收集30份血液和粪便样本.使用酶联免疫吸附测定(ELISA)法测定肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)的水平;用流式细胞仪测定CD4+ T淋巴细胞数目;采用实时定量聚合酶链式反应(qRT-PCR)法检测双歧杆菌、乳酸菌、大肠杆菌、粪肠球菌和屎肠球菌的mRNA水平;采用Spearman分析肠道菌群、炎症因子与CD4+ T淋巴细胞之间的相关性.
结论:不但AIDS/HIV患者体内肠道菌群紊乱,而且肠道菌群数量与CD4+ T淋巴细胞数量以及TNF-α、IL-6水平存在相关性.

关键词:获得性免疫缺陷综合征;肿瘤坏死因子(TNF-α);白细胞介素-6(IL-6);肠道菌群;CD4+ T淋巴细胞

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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