CLC number: R512.91
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2019-08-08
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Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan. Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals[J]. Journal of Zhejiang University Science B, 2019, 20(10): 793-802.
@article{title="Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals",
author="Jing Lu, Sai-Sai Ma, Wei-Ying Zhang, Jian-Ping Duan",
journal="Journal of Zhejiang University Science B",
volume="20",
number="10",
pages="793-802",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900075"
}
%0 Journal Article
%T Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals
%A Jing Lu
%A Sai-Sai Ma
%A Wei-Ying Zhang
%A Jian-Ping Duan
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 10
%P 793-802
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900075
TY - JOUR
T1 - Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals
A1 - Jing Lu
A1 - Sai-Sai Ma
A1 - Wei-Ying Zhang
A1 - Jian-Ping Duan
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 10
SP - 793
EP - 802
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900075
Abstract: Objective: In this study, we investigated the changes in peripheral blood inflammatory factors and intestinal flora in )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>acquired immune deficiency syndrome (AIDS) and human immunodeficiency virus (HIV)-positive individuals (AIDS/HIV patients), and explored the relationships among intestinal flora, peripheral blood inflammatory factors, and CD4+ T lymphocytes. Methods: Thirty blood and stool samples from an AIDS group and a control group were collected. The levels of tumor necrosis factor-α; (TNF-α;) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA), and the number of CD4+ T lymphocytes by a FACSCount automated instrument. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the messenger RNA (mRNA) levels of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis, and Enterococcus faecium. Correlations among intestinal flora, inflammatory factor levels, and CD4+ T lymphocyte values were evaluated using the Spearman correlation coefficient. Results: The levels of TNF-α and IL-6 in the AIDS group were higher than those in the control group, while the number of CD4+ T lymphocytes was lower. The amounts of Bifidobacterium and Lactobacillus in the AIDS group were significantly lower than those in control group, while the amounts of E. coli, E. faecalis, and E. faecium were much higher. The amounts of Bifidobacterium and Lactobacillus were negatively correlated with the content of TNF-α and IL-6 and the CD4+ T lymphocyte count, while those correlations were reversed for E. coli, E. faecalis, and E. faecium. Conclusions: The intestinal microbiota of AIDS/HIV patients were disordered, and there was a correlation between the amount of intestinal flora and the number of CD4+ T lymphocytes and the levels of TNF-α and IL-6.
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