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 ORCID:

Hui CHEN

https://orcid.org/0000-0001-9185-1764

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.8 P.682-694

http://doi.org/10.1631/jzus.B2000697


Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner


Author(s):  Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, Hui CHEN

Affiliation(s):  College of Life Science, Sichuan Agricultural University, Yaan 625014, China; more

Corresponding email(s):   chenhui@sicau.edu.cn

Key Words:  Hemin, Saponin, Conyza blinii, Heme oxygenase, Abiotic stress


Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, Hui CHEN. Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner[J]. Journal of Zhejiang University Science B, 2021, 22(8): 682-694.

@article{title="Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner",
author="Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, Hui CHEN",
journal="Journal of Zhejiang University Science B",
volume="22",
number="8",
pages="682-694",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000697"
}

%0 Journal Article
%T Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner
%A Tianrun ZHENG
%A Junyi ZHAN
%A Ming YANG
%A Maojia WANG
%A Wenjun SUN
%A Zhi SHAN
%A Hui CHEN
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 8
%P 682-694
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000697

TY - JOUR
T1 - Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner
A1 - Tianrun ZHENG
A1 - Junyi ZHAN
A1 - Ming YANG
A1 - Maojia WANG
A1 - Wenjun SUN
A1 - Zhi SHAN
A1 - Hui CHEN
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 8
SP - 682
EP - 694
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000697


Abstract: 
hemin can improve the stress resistance of plants through the heme oxygenase system. Additionally, substances contained in plants, such as secondary metabolites, can improve stress resistance. However, few studies have explored the effects of hemin on secondary metabolite content. Therefore, the effects of hemin on saponin synthesis and the mechanism of plant injury relief by hemin in Conyza blinii were investigated in this study. hemin treatment promoted plant growth and increased the antioxidant enzyme activity and saponin content of C. blinii under osmotic stress and cold stress. Further study showed that hemin could provide sufficient precursors for saponin synthesis by improving the photosynthetic capacity of C. blinii and increasing the gene expression of key enzymes in the saponin synthesis pathway, thus increasing the saponin content. Moreover, the promotion effect of hemin on saponin synthesis is dependent on heme oxygenase-1 and can be reversed by the inhibitor Zn-protoporphyrin-IX (ZnPPIX). This study revealed that hemin can increase the saponin content of C. blinii and alleviate the damage caused by abiotic stress, and it also broadened the understanding of the relationship between hemin and secondary metabolites in plant abiotic stress relief.

氯化血红素通过血红素加氧酶-1依赖方式诱导皂甙含量增加,有助于减轻渗透和冷胁迫对金龙胆草的损伤

目的:研究氯化血红素对金龙胆草皂苷含量的影响,以及与植物非生物胁迫的相关性。
创新点:通过施加外源氯化血红素研究金龙胆草中皂苷的变化与冷胁迫或渗透胁迫的相关性。
方法:在渗透胁迫和低温胁迫下,施加外源氯化血红素及其抑制剂,检测并分析金龙胆草多项生理指标、皂苷含量差异和关键基因表达量差异。
结论:氯化血红素能通过依赖血红素加氧酶-1的调控方式,提高金龙胆草皂苷的含量,从而减轻非生物胁迫对金龙胆草的伤害。

关键词:氯化血红素;皂苷;金龙胆草;血红素加氧酶;非生物胁迫

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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