Full Text:   <442>

Summary:  <46>

Suppl. Mater.: 

CLC number: 

On-line Access: 2022-05-13

Received: 2021-09-15

Revision Accepted: 2022-01-27

Crosschecked: 2022-05-13

Cited: 0

Clicked: 540

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhaoyang ZHANG

https://orcid.org/0000-0003-0288-7103

Fei MAO

https://orcid.org/0000-0001

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.5 P.423-431

http://doi.org/10.1631/jzus.B2100793


HucMSC-Ex alleviates inflammatory bowel disease via the lnc78583-mediated miR3202/HOXB13 pathway


Author(s):  Yuting XU, Li ZHANG, Dickson Kofi Wiredu OCANSEY, Bo WANG, Yilin HOU, Rong MEI, Yongmin YAN, Xu ZHANG, Zhaoyang ZHANG, Fei MAO

Affiliation(s):  Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang 212013, China; more

Corresponding email(s):   Zhangcy19791216@163.com, maofei2003@ujs.edu.cn

Key Words:  Inflammatory bowel disease, Mesenchymal stem cell-derived exosome, Long non-coding RNAs, Homeobox B13, miR3202


Yuting XU, Li ZHANG, Dickson Kofi Wiredu OCANSEY, Bo WANG, Yilin HOU, Rong MEI, Yongmin YAN, Xu ZHANG, Zhaoyang ZHANG, Fei MAO. HucMSC-Ex alleviates inflammatory bowel disease via the lnc78583-mediated miR3202/HOXB13 pathway[J]. Journal of Zhejiang University Science B, 2022, 23(5): 423-431.

@article{title="HucMSC-Ex alleviates inflammatory bowel disease via the lnc78583-mediated miR3202/HOXB13 pathway",
author="Yuting XU, Li ZHANG, Dickson Kofi Wiredu OCANSEY, Bo WANG, Yilin HOU, Rong MEI, Yongmin YAN, Xu ZHANG, Zhaoyang ZHANG, Fei MAO",
journal="Journal of Zhejiang University Science B",
volume="23",
number="5",
pages="423-431",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100793"
}

%0 Journal Article
%T HucMSC-Ex alleviates inflammatory bowel disease via the lnc78583-mediated miR3202/HOXB13 pathway
%A Yuting XU
%A Li ZHANG
%A Dickson Kofi Wiredu OCANSEY
%A Bo WANG
%A Yilin HOU
%A Rong MEI
%A Yongmin YAN
%A Xu ZHANG
%A Zhaoyang ZHANG
%A Fei MAO
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 5
%P 423-431
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100793

TY - JOUR
T1 - HucMSC-Ex alleviates inflammatory bowel disease via the lnc78583-mediated miR3202/HOXB13 pathway
A1 - Yuting XU
A1 - Li ZHANG
A1 - Dickson Kofi Wiredu OCANSEY
A1 - Bo WANG
A1 - Yilin HOU
A1 - Rong MEI
A1 - Yongmin YAN
A1 - Xu ZHANG
A1 - Zhaoyang ZHANG
A1 - Fei MAO
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 5
SP - 423
EP - 431
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100793


Abstract: 
As a group of nonspecific inflammatory diseases affecting the intestine, inflammatory bowel disease (IBD) exhibits the characteristics of chronic recurring inflammation, and was proven to be increasing in incidence (Kaplan, 2015). IBD induced by genetic background, environmental changes, immune functions, microbial composition, and toxin exposures (Sasson et al., 2021) primarily includes ulcerative colitis (UC) and Crohn's disease (CD) with complicated clinical symptoms featured by abdominal pain, diarrhea, and even blood in stools (Fan et al., 2021; Huang et al., 2021). UC is mainly limited to the rectum and the colon, while CD usually impacts the terminal ileum and colon in a discontinuous manner (Ordás et al., 2012; Panés and Rimola, 2017). In recent years, many studies have suggested the lack of effective measures in the diagnosis and treatment of IBD, prompting an urgent need for new strategies to understand the mechanisms of and offer promising therapies for IBD.

HucMSC-Ex通过调控lnc78583介导的miR3202/HOXB13通路缓解炎症性肠病

概要:炎症性肠病(IBD)是一组慢性、复发性肠道炎症,主要包括溃疡性结肠炎(UC)和克罗恩病(CD)。IBD的发病机制很复杂,其潜在机制仍不清楚。近年来,人们对长链非编码RNA(lncRNA)进行了研究,并将其确定为IBD的潜在生物标志物。在本研究中,通过使用高通量RNA测序筛选出一种新的lncRNA-lnc78583。前期荧光定量聚合酶链式反应(qRT-PCR)分析结果表明,lnc78583和同源框B13(HOXB13)的mRNA表达水平在IBD患者和炎症状态下的人结直肠黏膜上皮细胞(FHC)中显著降低。为了探索lnc78583在肠道炎症环境中的作用机制,我们通过对FHC进行lnc78583过表达处理,发现不仅增加了HOXB13和IL-10的表达,而且增强了细胞活力,降低了LDH的释放和p-NF-κB的激活。随后,我们用人脐带间充质干细胞来源的外泌体(hucMSC-Ex)处理FHC,结果表明其上调了lnc78583和HOXB13的表达。此外,进一步预测到miR3202在FHC中和lnc78583以及HOXB13呈负相关作用。lnc78583的过表达下调miR3202,而miR3202模拟物显着降低HOXB13的表达水平。综上,我们的研究初步证明hucMSC-Ex通过lnc78583介导的miR3202/HOXB13通路调节IBD的炎症进程,从而为IBD提供新的诊断方案和治疗策略。

关键词:炎症性肠病;长链非编码RNA(lncRNA);HOXB13;miR3202;间充质干细胞;外泌体

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]BarnhoornM, de Jonge-MullerE, MolendijkI, et al., 2018. Endoscopic administration of mesenchymal stromal cells reduces inflammation in experimental colitis. Inflamm Bowel Dis, 24(8):1755-1767.

[2]EstellerM, 2011. Non-coding RNAs in human disease. Nat Rev Genet, 12(12):861-874.

[3]FanXX, XiaQM, ZhangYY, et al., 2021. Aggregation-induced emission (AIE) nanoparticles-assisted NIR-II fluorescence imaging-guided diagnosis and surgery for inflammatory bowel disease (IBD). Adv Healthc Mater, 10(24):2101043.

[4]GanL, LvL, LiaoST, 2019. Long non-coding RNA H19 regulates cell growth and metastasis via the miR-22-3p/Snail1 axis in gastric cancer. Int J Oncol, 54(6):2157-2168.

[5]HuangLJ, MaoXT, LiYY, et al., 2021. Multiomics analyses reveal a critical role of selenium in controlling T cell differentiation in Crohn’s disease. Immunity, 54(8):‍‍1728-1744.E7.

[6]HuangZW, LeiW, HuHB, et al., 2018. H19 promotes non-small-cell lung cancer (NSCLC) development through STAT3 signaling via sponging miR-17. J Cell Physiol, 233(10):6768-6776.

[7]JatharS, KumarV, SrivastavaJ, et al., 2017. Technological Developments in lncRNA Biology. In: Rao MRS (Ed.), Long Non Coding RNA Biology. Springer, Singapore, p.283-323.

[8]JiaHY, LiuWZ, ZhangB, et al., 2018. HucMSC exosomes-delivered 14-3-3ζ enhanced autophagy via modulation of ATG16L in preventing cisplatin-induced acute kidney injury. Am J Transl Res, 10(1):101-113.

[9]JiangJJ, PiaoXY, HuSY, et al., 2020. LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/β-catenin signaling pathway. Aging (Albany NY), 12(10):8820-8836.

[10]KaplanGG, 2015. The global burden of IBD: from 2015 to 2025. Nat Rev Gastroenterol Hepatol, 12(12):720-727.

[11]LiuLS, LuoFY, LeiKB, 2021. Exosomes containing LINC00636 inhibit MAPK1 through the miR-450a-2-3p overexpression in human pericardial fluid and improve cardiac fibrosis in patients with atrial fibrillation. Mediators Inflamm, 2021:9960241.

[12]LiuR, TangAL, WangXY, et al., 2018. Inhibition of lncRNA NEAT1 suppresses the inflammatory response in IBD by modulating the intestinal epithelial barrier and by exosome-mediated polarization of macrophages. Int J Mol Med, 42(5):2903-2913.

[13]LucafòM, PugnettiL, BramuzzoM, et al., 2019. Long non-coding RNA GAS5 and intestinal MMP2 and MMP9 expression: a translational study in pediatric patients with IBD. Int J Mol Sci, 20(21):5280.

[14]OrdásI, EckmannL, TalaminiM, et al., 2012. Ulcerative colitis. Lancet, 380(9853):1606-1619.

[15]PaduaD, Mahurkar-JoshiS, LawIKM, et al., 2016. A long noncoding RNA signature for ulcerative colitis identifies IFNG-AS1 as an enhancer of inflammation. Am J Physiol Gastrointest Liver Physiol, 311(3):G446-G457.

[16]PanésJ, RimolaJ, 2017. Perianal fistulizing Crohn’s disease: pathogenesis, diagnosis and therapy. Nat Rev Gastroenterol Hepatol, 14(11):652-664.

[17]QianXY, ZhaoJY, YeungPY, et al., 2019. Revealing lncRNA structures and interactions by sequencing-based approaches. Trends Biochem Sci, 44(1):33-52.

[18]QiaoYQ, HuangML, XuAT, et al., 2013. LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn’s disease. J Biomed Sci, 20:87.

[19]SassonAN, IngramRJM, ZhangZX, et al., 2021. The role of precision nutrition in the modulation of microbial composition and function in people with inflammatory bowel disease. Lancet Gastroenterol Hepatol, 6(9):754-769.

[20]WangHT, MaP, LiuPP, et al., 2021. lncRNA SNHG6 promotes hepatocellular carcinoma progression by interacting with HNRNPL/PTBP1 to facilitate SETD7/LZTFL1 mRNA destabilization. Cancer Lett, 520:121-131.

[21]WuF, HuangY, DongFS, et al., 2016. Ulcerative colitis-associated long noncoding RNA, BC012900, regulates intestinal epithelial cell apoptosis. Inflamm Bowel Dis, 22(4):782-795.

[22]YaraniR, MirzaAH, KaurS, et al., 2018. The emerging role of lncRNAs in inflammatory bowel disease. Exp Mol Med, 50(12):1-14.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE