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On-line Access: 2023-03-10

Received: 2022-08-29

Revision Accepted: 2022-12-21

Crosschecked: 2023-03-13

Cited: 0

Clicked: 799

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Ruolang PAN

https://orcid.org/0000-0002-0810-3392

Yan YU

https://orcid.org/0000-0003-0723-5876

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.3 P.262-268

http://doi.org/10.1631/jzus.B2200436


Estrogen upregulates DNA2 expression through the PI3K-AKT pathway in endometrial carcinoma


Author(s):  Xinyan WANG, Xiuling XU, Ting ZHANG, Yang JIN, Sheng XU, Lifeng CHEN, Yucheng LAI, Ling ZHANG, Ruolang PAN, Yan YU

Affiliation(s):  Department of Gynecology, Zhejiang Provincial Hospital of Traditional Chinese Medicine, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China; more

Corresponding email(s):   panrl@zju.edu.cn, m05yuyan1@zju.edu.cn

Key Words:  Endometrial cancer, Estrogen receptor, DNA2, PI3K-AKT


Xinyan WANG, Xiuling XU, Ting ZHANG, Yang JIN, Sheng XU, Lifeng CHEN, Yucheng LAI, Ling ZHANG, Ruolang PAN, Yan YU. Estrogen upregulates DNA2 expression through the PI3K-AKT pathway in endometrial carcinoma[J]. Journal of Zhejiang University Science B, 2023, 24(3): 262-268.

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author="Xinyan WANG, Xiuling XU, Ting ZHANG, Yang JIN, Sheng XU, Lifeng CHEN, Yucheng LAI, Ling ZHANG, Ruolang PAN, Yan YU",
journal="Journal of Zhejiang University Science B",
volume="24",
number="3",
pages="262-268",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200436"
}

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%T Estrogen upregulates DNA2 expression through the PI3K-AKT pathway in endometrial carcinoma
%A Xinyan WANG
%A Xiuling XU
%A Ting ZHANG
%A Yang JIN
%A Sheng XU
%A Lifeng CHEN
%A Yucheng LAI
%A Ling ZHANG
%A Ruolang PAN
%A Yan YU
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%I Zhejiang University Press & Springer
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A1 - Sheng XU
A1 - Lifeng CHEN
A1 - Yucheng LAI
A1 - Ling ZHANG
A1 - Ruolang PAN
A1 - Yan YU
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Abstract: 
endometrial cancer is the most common gynecological malignancy, affecting up to 3% of women at some point during their lifetime (Morice et al., 2016; Li and Wang, 2021). Based on the pathogenesis and biological behavioral characteristics, endometrial cancer can be divided into estrogen-dependent (I) and non-estrogen-dependent (II) types (Ulrich, 2011). Type I accounts for approximately 80% of cases, of which the majority are endometrioid carcinomas, and the remaining are mucinous adenocarcinomas (Setiawan et al., 2013). It is generally recognized that long-term stimulation by high estrogen levels with the lack of progesterone antagonism is the most important risk factor; meanwhile, there is no definite conclusion on the specific pathogenesis. The incidence of endometrial cancer has been on the rise during the past two decades (Constantine et al., 2019; Gao et al., 2022; Luo et al., 2022). Moreover, the development of assisted reproductive technology and antiprogestin therapy following breast cancer surgery has elevated the risk of developing type I endometrial cancer to a certain extent (Vassard et al., 2019). Therefore, investigating the influence of estrogen in type I endometrial cancer may provide novel concepts for risk assessment and adjuvant therapy, and at the same time, provide a basis for research on new drugs to treat endometrial cancer.

雌激素通过PI3K-AKT通路上调子宫内膜癌中DNA2的表达

王鑫炎1,徐秀玲2,张婷1,金央2,徐盛3,陈莉锋3,赖雨程3,张玲4,5,潘若浪4,5,余艳3
1浙江省中医院, 浙江中医药大学第一附属医院妇科, 中国杭州市, 310006
2浙江中医药大学附属杭州市中医院, 杭州市中医院妇产科, 中国杭州市, 310005
3浙江省人民医院,杭州医学院附属人民医院妇科, 中国杭州市, 310014
4浙江省细胞药物与应用技术研究重点实验室, 中国杭州市, 311122
5浙江省易文赛细胞药物和制品研究院, 中国杭州市, 311122
概要: 子宫内膜癌是最常见的子宫癌类型,占子宫癌病例的90%。本文通过临床研究,检测子宫内膜癌患者标本中雌激素受体(ER)和DNA复制ATP依赖性解旋酶/核酸酶(DNA2)的表达,并分析两者之间的关系。通过细胞培养实验研究ER调控DNA2表达的机制,证实其与PI3K-AKT通路有关。研究还发现,使用短发夹RNA(shRNA)特异性靶向降低Ishikawa中DNA2的表达,会导致细胞增殖和克隆形成能力降低。总的来说,本研究证实了DNA2作为治疗靶点的可行性,并证明抑制DNA2可使Ishikawa对喜树碱(CPT)化疗增敏。我们的发现为DNA2的潜在机制提供了新的见解,这将有助于开发与子宫内膜癌诊断和治疗相关的新方法。

关键词:子宫内膜癌;雌激素受体;DNA2;PI3K-AKT通路

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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