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On-line Access: 2023-05-15

Received: 2022-09-04

Revision Accepted: 2023-01-15

Crosschecked: 2023-05-16

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Chuanxin CHEN


Yuyu NIU


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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.5 P.418-429


Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines

Author(s):  Shuang LI, Zhenzhen CHEN, Chuanxin CHEN, Yuyu NIU

Affiliation(s):  State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China; more

Corresponding email(s):   redpanda00@126.com, niuyy@lpbr.cn

Key Words:  Monkey pluripotent stem cell, Multiplexed single-cell sequencing, Naive pluripotency, Extended pluripotent stem cells

Shuang LI, Zhenzhen CHEN, Chuanxin CHEN, Yuyu NIU. Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines[J]. Journal of Zhejiang University Science B, 2023, 24(5): 418-429.

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publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
%A Shuang LI
%A Zhenzhen CHEN
%A Chuanxin CHEN
%A Yuyu NIU
%J Journal of Zhejiang University SCIENCE B
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%DOI 10.1631/jzus.B2200440

T1 - Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
A1 - Shuang LI
A1 - Zhenzhen CHEN
A1 - Chuanxin CHEN
A1 - Yuyu NIU
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 5
SP - 418
EP - 429
%@ 1673-1581
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200440

Efforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human primate cells. Here, we introduce a multiplexed single-cell sequencing technique to profile the molecular features of monkey pluripotent stem cells in published culture conditions. The results demonstrate suboptimized maintenance of pluripotency and show that the selected signaling pathways for resetting human stem cells can also be interpreted for establishing monkey cell lines. Overall, this work legitimates the translation of novel human cell line culture conditions to monkey cells and provides guidance for exploring chemical cocktails for monkey stem cell line derivation.


1昆明理工大学, 省部共建非人灵长类生物医学国家重点实验室, 中国昆明市, 650500
2昆明理工大学生命科学与技术学院, 中国昆明市, 650500
3云南省灵长类生物医学重点实验室, 中国昆明市, 650500
4生物岛实验室, 中国广州市, 510005
5广州国家实验室, 中国广州市, 510005


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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