CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2024-03-06
Cited: 0
Clicked: 1122
Fulong NAN, Wenlong NAN, Xin YAN, Hui WANG, Shasha JIANG, Shuyun ZHANG, Zhongjie YU, Xianjuan ZHANG, Fengjun LIU, Jun LI, Xiaoqiong ZHOU, Delei NIU, Yiquan LI, Wei WANG, Ning SHI, Ningyi JIN, Changzhan XIE, Xiaoni CUI, He ZHANG, Bin WANG, Huijun LU. Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells[J]. Journal of Zhejiang University Science B, 2024, 25(3): 254-270.
@article{title="Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells",
author="Fulong NAN, Wenlong NAN, Xin YAN, Hui WANG, Shasha JIANG, Shuyun ZHANG, Zhongjie YU, Xianjuan ZHANG, Fengjun LIU, Jun LI, Xiaoqiong ZHOU, Delei NIU, Yiquan LI, Wei WANG, Ning SHI, Ningyi JIN, Changzhan XIE, Xiaoni CUI, He ZHANG, Bin WANG, Huijun LU",
journal="Journal of Zhejiang University Science B",
volume="25",
number="3",
pages="254-270",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300134"
}
%0 Journal Article
%T Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells
%A Fulong NAN
%A Wenlong NAN
%A Xin YAN
%A Hui WANG
%A Shasha JIANG
%A Shuyun ZHANG
%A Zhongjie YU
%A Xianjuan ZHANG
%A Fengjun LIU
%A Jun LI
%A Xiaoqiong ZHOU
%A Delei NIU
%A Yiquan LI
%A Wei WANG
%A Ning SHI
%A Ningyi JIN
%A Changzhan XIE
%A Xiaoni CUI
%A He ZHANG
%A Bin WANG
%A Huijun LU
%J Journal of Zhejiang University SCIENCE B
%V 25
%N 3
%P 254-270
%@ 1673-1581
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300134
TY - JOUR
T1 - Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells
A1 - Fulong NAN
A1 - Wenlong NAN
A1 - Xin YAN
A1 - Hui WANG
A1 - Shasha JIANG
A1 - Shuyun ZHANG
A1 - Zhongjie YU
A1 - Xianjuan ZHANG
A1 - Fengjun LIU
A1 - Jun LI
A1 - Xiaoqiong ZHOU
A1 - Delei NIU
A1 - Yiquan LI
A1 - Wei WANG
A1 - Ning SHI
A1 - Ningyi JIN
A1 - Changzhan XIE
A1 - Xiaoni CUI
A1 - He ZHANG
A1 - Bin WANG
A1 - Huijun LU
J0 - Journal of Zhejiang University Science B
VL - 25
IS - 3
SP - 254
EP - 270
%@ 1673-1581
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300134
Abstract: As a potential vectored vaccine, newcastle disease virus (NDV) has been subject to various studies for vaccine development, while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation. To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells (DCs) and t cells, DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide (LPS) for further detection by enzyme-linked immunosorbent assay (ELISA), flow cytometry, immunoblotting, and quantitative real-time polymerase chain reaction (qRT-PCR). The results revealed that NDV infection resulted in the inhibition of interleukin (IL)-12p40 in DCs through a p38 mitogen-activated protein kinase (MAPK)-dependent manner, thus inhibiting the synthesis of IL-12p70, leading to the reduction in T cell proliferation and the secretion of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and IL-6 induced by DCs. Consequently, downregulated cytokines accelerated the infection and viral transmission from DCs to t cells. Furthermore, several other strains of NDV also exhibited inhibitory activity. The current study reveals that NDV can modulate the intensity of the innate‒adaptive immune cell crosstalk critically toward viral invasion improvement, highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine.
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