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On-line Access: 2023-11-14

Received: 2023-03-21

Revision Accepted: 2023-05-12

Crosschecked: 2023-11-15

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Li Jan LO


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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.11 P.1037-1046


Loss-of-function of zebrafish cdt1 causes retarded body growth and underdeveloped gonads resembling human Meier-Gorlin syndrome

Author(s):  Yinan HE, Yong WANG, Yanqing ZHU, Li Jan LO

Affiliation(s):  MOE Key Laboratory for Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   g0403022@zju.edu.cn

Key Words:  Zebrafish, cdt1-201 cdt1-202, Dwarfism, Infertility, Unique N-terminal in bony fish

Yinan HE, Yong WANG, Yanqing ZHU, Li Jan LO. Loss-of-function of zebrafish cdt1 causes retarded body growth and underdeveloped gonads resembling human Meier-Gorlin syndrome[J]. Journal of Zhejiang University Science B, 2023, 24(11): 1037-1046.

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author="Yinan HE, Yong WANG, Yanqing ZHU, Li Jan LO",
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publisher="Zhejiang University Press & Springer",

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%T Loss-of-function of zebrafish cdt1 causes retarded body growth and underdeveloped gonads resembling human Meier-Gorlin syndrome
%A Yinan HE
%A Yong WANG
%A Yanqing ZHU
%A Li Jan LO
%J Journal of Zhejiang University SCIENCE B
%V 24
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%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300195

T1 - Loss-of-function of zebrafish cdt1 causes retarded body growth and underdeveloped gonads resembling human Meier-Gorlin syndrome
A1 - Yinan HE
A1 - Yong WANG
A1 - Yanqing ZHU
A1 - Li Jan LO
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 11
SP - 1037
EP - 1046
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2300195

The cell cycle consists of four distinct phases: G0/G1, S (DNA synthesis), G2, and M (mitosis). The G1 to S transition is typified by an accumulation of 4',?6-diamidino-2-phenylindole (DAPI) signal that indicates the rapid DNA synthesis initiated at special sites on DNA, generally called the Ori (origin of replication) (Alfa et al., 1989). The cell division cycle 10 (Cdc10)?-dependent transcript 1 (Cdt1) is bestowed the term replication origin licensing factor for its role in warranting replication once (and only once) per round of the eukaryotic cell cycle, during the S phase (Pozo and Cook, 2017). In a normal cell cycle, Cdt1 is present only in the G1 and S entry phases, whereas Geminin, a protein that targets Cdt1 for S-phase-dependent proteolysis, is present in the S and G2 phases. Failure of this gatekeeping task as observed in cdt1 overexpression, for example in yeast, leads to inappropriate origin firing (also termed re-replication (Vaziri et al., 2003)) and eventually DNA damage checkpoint activation (Kanellou et al., 2020).




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