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Journal of Zhejiang University SCIENCE B 2024 Vol.25 No.9 P.773-788

http://doi.org/10.1631/jzus.B2300449


Comparison of nitrification inhibitors for mitigating cadmium accumulation in pakchoi and their associated microbial mechanisms


Author(s):  Wenxin DU, Qingyang ZHU, Xiangting JING, Weijie HU, Yao ZHUANG, Yijie JIANG, Chongwei JIN

Affiliation(s):  State Key Laboratory of Plant Environmental Resilience, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   21914114@zju.edu.cn, jincw@zju.edu.cn

Key Words:  Cadmium (Cd), Nitrification inhibitor, Soil microbial structure, Safe crop production


Wenxin DU, Qingyang ZHU, Xiangting JING, Weijie HU, Yao ZHUANG, Yijie JIANG, Chongwei JIN. Comparison of nitrification inhibitors for mitigating cadmium accumulation in pakchoi and their associated microbial mechanisms[J]. Journal of Zhejiang University Science B, 2024, 25(9): 773-788.

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author="Wenxin DU, Qingyang ZHU, Xiangting JING, Weijie HU, Yao ZHUANG, Yijie JIANG, Chongwei JIN",
journal="Journal of Zhejiang University Science B",
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number="9",
pages="773-788",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300449"
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%T Comparison of nitrification inhibitors for mitigating cadmium accumulation in pakchoi and their associated microbial mechanisms
%A Wenxin DU
%A Qingyang ZHU
%A Xiangting JING
%A Weijie HU
%A Yao ZHUANG
%A Yijie JIANG
%A Chongwei JIN
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
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T1 - Comparison of nitrification inhibitors for mitigating cadmium accumulation in pakchoi and their associated microbial mechanisms
A1 - Wenxin DU
A1 - Qingyang ZHU
A1 - Xiangting JING
A1 - Weijie HU
A1 - Yao ZHUANG
A1 - Yijie JIANG
A1 - Chongwei JIN
J0 - Journal of Zhejiang University Science B
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SP - 773
EP - 788
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300449


Abstract: 
The use of nitrification inhibitors has been suggested as a strategy to decrease cadmium (Cd) accumulation in crops. However, the most efficient nitrification inhibitor for mitigating crop Cd accumulation remains to be elucidated, and whether and how changes in soil microbial structure are involved in this process also remains unclear. To address these questions, this study applied three commercial nitrification inhibitors, namely, dicyandiamide (DCD), 3,4-dimethylpyrazole phosphate (DMPP), and nitrapyrin (NP), to pakchoi. The results showed that both DCD and DMPP (but not NP) could efficiently decrease Cd concentrations in pakchoi in urea- and ammonium-fertilized soils. In addition, among the three tested nitrification inhibitors, DMPP was the most efficient in decreasing the Cd concentration in pakchoi. The nitrification inhibitors decreased pakchoi Cd concentrations by suppressing acidification-induced Cd availability and reshaping the soil microbial structure; the most effective nitrification inhibitor was DMPP. Ammonia oxidation generates the most protons during nitrification and is inhibited by nitrification inhibitors. Changes in environmental factors and predatory bacterial abundance caused by the nitrification inhibitors changed the soil microbial structure and increased the potential participants in plant Cd accumulation. In summary, our study identified DMPP as the most efficient nitrification inhibitor for mitigating crop Cd contamination and observed that the soil microbial structural changes caused by the nitrification inhibitors contributed to decreasing Cd concentration in pakchoi.

减控小青菜镉污染的高效硝化抑制剂筛选及其微生物学机制研究

杜闻昕, 朱清扬, 景湘婷, 胡伟杰, 庄瑶, 蒋逸捷, 金崇伟
浙江大学植物抗逆高效全国重点实验室, 中国杭州市, 310058
摘要:氮肥配施硝化抑制剂是一种能有效降低作物镉积累的策略。目前有多种市售硝化抑制剂,但何种硝化抑制剂具有最佳的作物镉积累减控效果及其具体作用机制尚不清楚。本研究对双氰胺(DCD)、3,4-二甲基吡唑磷酸盐(DMPP)和三氯甲基吡啶三种常用商品化硝化抑制剂减控小青菜镉污染的效果进行对比。结果表明,在施用尿素和铵态氮肥时,配施DCD和DMPP均能有效降低小青菜可食用部位镉浓度,其中DMPP效果最佳,而配施三氯甲基吡啶则无显著效果。进一步研究发现,硝化抑制剂主要通过抑制硝化作用释放质子引起的镉活化降低小青菜镉含量。此外,结构方程分析表明,土壤微生物群落结构的重塑同样在硝化抑制剂减控小青菜镉积累中发挥作用。

关键词:镉;硝化抑制剂;土壤微生物结构;作物安全生产

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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