JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

An automated raw tissue precision slicing system for methodological advances in biomedical applications: Streamlining decellularization in porcine cornea-derived tissue-specific bioink fabrication and beyond

Author(s): Won Bin Choi , Yeon-ju Lee , Ju Young Park, Jinah Jang, Wan Kyun Chung
Affiliation(s): Department of Mechanical Engineering, Pohang University of Science and Technology; more
Corresponding email(s): jinahjang@postech.ac.kr
Key Words: raw tissue slicing, submillimeter slices, high precision, oscillatory incision, applanation, decellularization

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Won Bin Choi , Yeon-ju Lee , Ju Young Park, Jinah Jang, Wan Kyun Chung. An automated raw tissue precision slicing system for methodological advances in biomedical applications: Streamlining decellularization in porcine cornea-derived tissue-specific bioink fabrication and beyond[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/bdm.2400212

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author="Won Bin Choi , Yeon-ju Lee , Ju Young Park, Jinah Jang, Wan Kyun Chung",
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doi="https://doi.org/10.1631/bdm.2400212"
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%T An automated raw tissue precision slicing system for methodological advances in biomedical applications: Streamlining decellularization in porcine cornea-derived tissue-specific bioink fabrication and beyond
%A Won Bin Choi
%A Yeon-ju Lee
%A Ju Young Park
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%A Wan Kyun Chung
%J Journal of Zhejiang University SCIENCE B
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doi="https://doi.org/10.1631/bdm.2400212"

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A1 - Won Bin Choi
A1 - Yeon-ju Lee
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A1 - Jinah Jang
A1 - Wan Kyun Chung
J0 - Journal of Zhejiang University Science B
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%@ 2095-9184
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doi="https://doi.org/10.1631/bdm.2400212"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The decellularized extracellular matrix constitutes a pivotal biomaterial created by decellularizing the natural extracellular matrix (ECM). This material serves as a supportive medium for intricate cellular interactions, fostering cell growth, differentiation, and organization. However, challenges persist in decellularization, necessitating a balance between preserving the structural integrity of the ECM and achieving effective cellular removal. One approach to enhancing decellularization involves pre-eliminating unnecessary tissues, effectively reducing final DNA levels to < 50 ng per mg of ECM on pre-processed tissues. Although this strategic step augments decellularization efficiency, the current manual execution method depends on operator skill. To address this limitation, we propose an automated raw tissue slicing system that does not require prior tissue preparation for slicing. Through carefully controlled tissue applanation pressure and oscillatory incisions with optimized parameters, the system achieved precision < � 10 �m in obtaining submillimeter tissue slices of the porcine cornea, while avoiding significant microscopic complications in the tissue structure, as observed by tissue histology. These findings suggest the system�s capability to streamline and automate preliminary tissue slicing operations. The efficacy of this approach for decellularization was validated by processing porcine corneas using the proposed system, and subsequently decellularizing the processed tissues. DNA level analysis revealed that sliced, subdivided tissues created by the proposed system could expedite DNA reduction even at the initial steps of the decellularization process, suggesting the promise of enhancing the overall decellularization procedure.

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