CLC number: S435.311
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-03-22
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C. O. Oladoye, I. F. Connerton, R. M. O. Kayode, P. F. Omojasola, I. B. Kayode. Biomolecular characterization, identification, enzyme activities of molds and physiological changes in sweet potatoes (Ipomea batatas) stored under controlled atmospheric conditions[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1400328 @article{title="Biomolecular characterization, identification, enzyme activities of molds and physiological changes in sweet potatoes (Ipomea batatas) stored under controlled atmospheric conditions", %0 Journal Article TY - JOUR
甘薯贮藏环境中霉菌的生物分子特征、鉴定和酶活性及甘薯生理变化的研究方法:从不同温度下保存的甘薯得到霉菌菌株,提取其细菌基因组DNA,进行聚合酶链反应(PCR)及测序鉴定。将甘薯分成扑海因处理组、次氯酸钠处理组和对照组,在为期三个月的贮藏时间内,对霉菌致病性、酶活性和空气条件对甘薯的生理变化的影响进行评估。 结论:实验结束后,扑海因处理组的变质率为5%,次氯酸钠组为55%,对照组为100%。研究发现,甘薯组织变质主要由于不同的微生物酶的活动,尤其是受感染组织的果胶酶活性。因此,建议将扑海因作为甘薯贮藏之前的保鲜剂。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
Reference[1]Acedo, A.L.Jr., Data, E.S., Quevedo, M.A., 1996. Genotype variations in quality and shelf life of fresh roots of Philippines sweet potato grown in two planting seasons. J. Sci. Food Agric., 72(2):209. [2]Afek, U., Orenstein, J., 2003. Decreased sweet potato decay during storage by steam treatments. Crop Prot., 22(2):321-324. [3]Afek, U., Orenstein, J., Nuriel, E., 1998. Increased quality and prolonged storage of sweet potatoes in Israel. Phytoparasitica, 26(4):307-312. [4]Afek, U., Orenstein, J., Nuriel, E., 1999. Fogging disinfectants inside storage rooms against pathogens of potato and sweet potatoes. Crop Prot., 18(2):111. [5]Aidoo, K.E., 1993. Post-harvest storage and preservation of tropical crops. Int. Biodeter. Biodegr., 32(1-3):161. [6]Ali, M.S., Bhuiyan, M.K.R., Manna, M.A., et al., 1991. Postharvest handling and utilization of sweet potato in Bangladesh. In: Dayal, T.R., Scott, G.J., Kurup, G.T., et al. (Eds.), Sweet Potato in South Asia: Postharvest Handling, Storage, Processing and Uses. International Potato Centre (CIP), Lima, Peru, p.13-22. [7]Amadioha, A.C., 1993. Production of cellulolytic enzymes by Rhizopus oryzae in culture and Rhizopus-infected tissues of potato tubers. Mycologia, 85(4):574-578. [8]Bennik, M.H.J., Vorstman, W., Smid, E.J., et al., 1998. The influence of oxygen and carbon dioxide on the growth of prevalent Enterobateriaceae and Pseudomonas species isolated from fresh and controlled-atmosphere stored vegetables. Food Microbiol., 15(5):459-469. [9]Bernfeld, P., 1955. Amylases, α and β. Meth. Enzymol., 1:149-158. [10]Borneman, J., Hartin, R.J., 2000. PCR primers that amplify fungi rRNA genes from environmental samples. Appl. Environ. Microbiol., 66(10):4356-4360. [11]Bourke, R.M., 1982. Sweet potato in Papua New Guinea. In: Villareal, R.L., Griggs, T.D. (Eds.), Sweet Potato. Proc. 1st Int. Symp. Asian Veg. Res. Dev. Center, Shanhua, Tainan, p.45-58. [12]Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2):248-254. [13]Byrde, R.J.W., 1979. Role of polysaccharide degrading enzymes in microbial pathogenicity. In: Berkeley, R.C.W., Gooday, G.W., Ellwood, D.C. (Eds.), Microbial Polysaccharides and Polysaccharases. Academic Press, London, p.417-436. [14]Cabanilla, L.S., 1996. Sweet Potato in the Philippines: Production, Processing and Future Prospects. International Potato Centre (CIP), Lima, Peru. [15]Chávez, R., Bull, P., Eyzaguirre, J., 2006. The xylanolytic enzyme system from the genus Penicillium. J. Biotechnol., 123(4):413-433. [16]Chesson, A., 1980. A review: maceration in relation to the post-harvest handling and processing of plant material. J. Appl. Microbiol., 48(1):1-45. [17]Chesson, A., Codner, T.L.R.C., 1978. The maceration of vegetable tissue by a strain of Bacillus subtilis. J. Appl. Microbiol., 44(3):347-364. [18]Clark, C.A., 1992. Postharvest diseases of sweet potatoes and their control. Postharv. News Inform., 3(4):75-79. [19]Clark, C.A., Dukes, P.D., Moyer, J.W., 1992. Disease. In: Jones, J., Bouwkamp, J.C. (Eds.), Fifty Years of Cooperative Sweet Potato Research, 1939-1989. Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center. [20]Cocolin, L., Aggio, D., Manzano, M., et al., 2002. An application of PCR-DGGE analysis to profile the yeast populations in raw milk. Int. Dairy J., 12(5):407-411. [21]Dayal, T.R., Mehra, S.K., Scott, G.J., 1991. Global perspective on sweet potato production and use. In: Dayal, T.R., Scott, G.J., Kurup, G.T., et al. (Eds.), Sweet Potato in South Asia: Postharvest Handling, Storage, Processing and Uses. International Potato Centre (CIP), Lima, Peru, p.1-12. [22]de Vries, R.P., van Kuyk, P.A., Kester, H.C., et al., 2002. The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds. Biochem. J., 363(2):377-386. [23]Doku, E.V., 1989. Root crops in Ethiopia. In: Alvares, M.N., Hahn, S.K. (Eds.), Root Crops and Low-Input Agriculture: Helping to Meet Food Self-sufficiency Goals in Eastern and Southern Africa. Proceeding 3rd Eastern and Southern Africa Regional Workshop on Root and Tuber Crops, Mzuzu, Malawi, p.125-132. [24]Donaghy, J., McKay, A.M., 1995. Production of feruloyl/ρ-coumaroyl esterase activity by Penicillium expansum, Penicillium brevicompactum and Aspergillus niger. J. Appl. Bacteriol., 79(6):657-666. [25]Eckert, J.W., Ogawa, J.M., 1988. The chemical control of post-harvest diseases: deciduous fruits, berries, vegetables and root/tuber crops. Annu. Rev. Phytopathol., 26(1):433-469. [26]Ekundayo, J.A., Daniel, T.M., 1973. Cassava rot and its control. Trans. Br. Mycol. Soc., 61(1):27-32. [27]FAO (Food and Agriculture Organization of the United Nations), 2002. Production Year Book. Rome, 56:163. [28]Fellows, P.J., 2000. Food Processing Technology: Principles and Practice. Wood Head Publishing Ltd., Cambridge, England, p.406-417. [29]Hall, M.R., 1993. Mid storage heating increased plant production from bedded sweet potato roots. HortScience, 28(8):780-781. [30]Hautala, J.A., Connerb, H., Jacobson, J.W., et al., 1977. Isolation and characterization of nuclei from Neurospora crassa. J. Bacteriol., 130(2):704-713. [31]Holmes, G.J., Stange, R.R., 2002. Influence of wound type and storage duration on susceptibility of sweet potato to Rhizopus soft rot. Plant Dis., 86(4):345. [32]Horton, D., Prain, G., Gregory, P., 1989. High Level Investment Returns for Global Sweet Potato Research and Development. CIP Circular, p.1-7. [33]Ikediagwu, E.O., Ejale, A.U., 1980. Root surface mycoflora of cassava (Manihot esculenta) and post-harvest rot of the tubers. Mycopathology, 71(2):67-71. [34]Jusuf, M., Widodo, Y., Antarlina, S.S., 1997. Problems and subject of sweet potato production and utilization in Indonesia. Proceedings of International Workshop on Sweet Potato Production System Toward the 21st Century, Miyakonojo, Miyazaki, Japan, p.83. [35]Karuri, E.G., Ojijo, N.K.O., 1994. Storage studies on sweet potato roots: experiences with KSP20 cultivar. Acta Hortic., 368:441-478. [36]Katan, T., 1982. Resistance to 3,5-dichlorophenyl-N-cyclic imide (‘dicarboximide’) fungicides in the grey mould pathogen Botrytis cinerea on protected crops. Plant Pathol., 31(2):133-141. [37]Kays, S.J., Bhagsari, A.S., Picha, D.H., 1992. Physiology and chemistry. In: Jones, A., Bouwkamp, J.C. (Eds.), Fifty Years of Cooperative Sweet Potato Research: 1939-1989. Southern Cooperative Series, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, p.369. [38]Kurup, G.T., Balagopalan, C., 1991. Sweet potato production, post-harvest handling and utilization in India. In: Dayal, T.R., Scott, G.J., Kurup, G.T., et al. (Eds.), Sweet Potato in South Asia: Postharvest Handling Storage, Processing and Uses. International Potato Centre (CIP), Lima, Peru, p.33-42. [39]Lim, G.E., Khew, E., Yeoh, H.H., 1985. Extracellular enzymes of some black Aspergilli in Singapore. MIRCEN J. Appl. Microbiol. Biotechnol., 1(1):55-61. [40]Mtunda, K., Chilosa, D., Rwiza, E., 2001. Damage reduces shelf-life of sweet potato during marketing. Afr. Crop Sci. J., 9(1):301-307. [41]Obi, S.K.C., Moneke, A.N., 1986. Pectinolytic and cellulolytic enzymes complex of fungi associated with soft rot of yams (Dioscorea rotundata Bir.). Int. Biodeter., 22(4):295-299. [42]Ogawa, J.M., Adaskaveg, J.E., Conn, K.E., 1992. Efficacy of iprodione wax/oil mixtures for control of postharvest decay of fruit caused by Rhizopus and Alternaria sp. Phytopathology, 82:1064. [43]Olorunda, A.O., 1979. Storage and processing of some Nigeria root crops. In: Plucknett D.L. (Ed.), Small-Scale Processing and Storage of Tropical Root Crops. West-View Press, Boulder, Colorado, p.95. [44]Onwueme, I.C., Charles, W.B., 1994. Tropical Root and Tuber Crops: Production, Perspective and Future Prospects. Food and Agriculture Organization, Rome, Italy, p.139-167. [45]Ragaert, P., Devlieghere, F., Debevere, J., 2007. Role of microbiological and physiological spoilage mechanisms during storage of minimally processed vegetables. Postharvest Biol. Tech., 44(3):185-194. [46]Ray, R.C., 2004. Cellulolytic enzymes production in cultures and fungi-infected sweet potato roots. Ann. Trop. Res., 23:34. [47]Ray, R.C., Misra, R.S., 1995. Spoilage of sweet potato tubers in tropics. I. Microorganisms associated with spoilage of sweet potato. Adv. Hortic. Sci., 8:159-163. [48]Ray, R.C., Balagopalan, C., 1997. Post-harvest Spoilage of Sweet Potato in Tuber Crops. Research Institute, Trivandrum, India, p.31. [49]Ray, R.C., Byju, G., 2003. Root surface culturable microflora associated with postharvest spoilage of sweet potato (Ipomea batatas L.). Adv. Hortic. Sci., 17(3):176-180. [50]Ray, R.C., Ravi, V., 2005. Post-harvest spoilage of sweet potato in tropics and control measures. Crit. Rev. Food Sci. Nutr., 45(7-8):623-644. [51]Ray, R.C., Misra, R.S., Ghose, P.K., 1997. Spoilage of sweet potato tubers in tropics. III. Soft rot by Rhizopus oryzae L. Adv. Hortic. Sci., 11(2):103. [52]Rees, D., Kapinga, R., Rwiza, E., 1998. The potential for extending the shelf-life of sweet potato in East Africa through cultivar selection. Trop. Agric., 75(2):208-211. [53]Rees, D., Kapinga, R., Mtunda, K., 2001. Effect of damage on market value and self-life of sweet potato in urban markets of Tanzania. Trop. Sci., 41:142-150. [54]Rees, D., van Oirschot., Q.E.A., Amour, R., et al., 2003. Cultivar variation in keeping quality of sweet potatoes. Postharvest Biol. Technol., 28(2):313-325. [55]Ritchie, D.F., 1982. Effect of dichloran, iprodione, procymidone, and vinclozolin on the mycelial growth, sporulation, and isolation of resistant strains of Monilinia fructicola. Plant Dis., 66(1):484-486. [56]Sholberg, P.L., Cliff, M., Moyls, A.L., 2001. Fumigation with acetic acid vapour to control decay of stored apples. Fruits, 56(5):355-366. [57]Sowley, E.N.K., Oduro, K.A., 2002. Effectiveness of curing in controlling fungal-induced storage rot in sweet potato in Ghana. Trop. Sci., 42:6. [58]Thankappan, M., 1994. Fungal diseases of sweet potatoes. In: Chadha, K.L., Nayar, G.G. (Eds.), Advances in Horticulture. Malhotra Publishing House, New Delhi, p.388. [59]Tian, S.P., 2006. Microbial control of post-harvest diseases of fruits and vegetables: current concepts and future outlook. In: Ray, R.C., Ward, O.P. (Eds.), Microbial Biotechnology in Horticulture. Science Publishers, Enfield, USA, p.163-202. [60]Wang, Y., Horvat, R.J., White, R.A., et al., 1998. Influence of post-harvest curing treatment on the synthesis of the volatile flavour components in sweet potato. Acta Hortic., 464:207-212. [61]Williams, C.N., Chew, W.Y., Rajaratnam, J.A., 1980. Tree and Field Crops of the Wetter Regions of the Tropics: Intermediate Tropical Agriculture Series. Longman, London, p.211-213. [62]Wisniewski, M.E., Wilson, C.L., 1992. Biological control of postharvest diseases of fruits and vegetables: recent advances. HortScience, 27:94-98. [63]Yoshihiro, U., Kazuo, C., 2007. Effects of short-term exposure to oxygen atmospheres on physiological responses of sweet potato roots. J. Jpn. Soc. Hortic. Sci., 76(3):258-265. Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou
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