Abstract: With the increasing occurrence of haze during the summer, the physicochemical characteristics and toxicity differences in PM_2.5 in different seasons are of great concern. Hangzhou is located in an area that has a subtropical monsoon climate where the humidity is very high during both the summer and winter. However, there are limited studies on the seasonal differences in PM_2.5 in these weather conditions. In this test, PM_2.5 samples were collected in the winter and summer, the morphology and chemical composition of PM_2.5 were analyzed, the toxicity of PM_2.5 to human bronchial cells BEAS-2B was compared, and the correlation between PM_2.5 toxicity and the chemical composition was discussed. The results showed that during both the winter and summer, the main compounds in the PM_2.5 samples were water-soluble ions, particularly SO₄²⁻, NO₃⁻, and NH₄⁺, followed by organic components, while heavy metals were present at lower levels. The higher the mass concentration of PM_2.5, the greater its impact on cell viability and ROS levels. However, when the mass concentration of PM_2.5 was similar, the water extraction from the summer samples showed a greater impact on BEAS-2B than that from the winter samples. The cytotoxicity of PM_2.5 was closely associated with heavy metals and organic pollutants but less related to water-soluble ions.

冬夏季PM_2.5的理化特征及其对人支气管细胞BEAS-2B的毒性

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