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Role of try and amy in the feeding, digestion, growth and development of the Japanese medaka (Oryzias latipes): insight from a comparative gene knockout study
Affiliation(s):
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China;
moreAffiliation(s): College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China; Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China;
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Xiaodan JIA, Shulin TANG, Hexiong FENG, Dimei XU, Chenyuan ZHU, Ke LU, Xufang LIANG. Role of try and amy in the feeding, digestion, growth and development of the Japanese medaka (Oryzias latipes): insight from a comparative gene knockout study[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400246
@article{title="Role of try and amy in the feeding, digestion, growth and development of the Japanese medaka (Oryzias latipes): insight from a comparative gene knockout study", author="Xiaodan JIA, Shulin TANG, Hexiong FENG, Dimei XU, Chenyuan ZHU, Ke LU, Xufang LIANG", journal="Journal of Zhejiang University Science B", year="in press", publisher="Zhejiang University Press & Springer", doi="https://doi.org/10.1631/jzus.B2400246" }
%0 Journal Article %T Role of try and amy in the feeding, digestion, growth and development of the Japanese medaka (Oryzias latipes): insight from a comparative gene knockout study %A Xiaodan JIA %A Shulin TANG %A Hexiong FENG %A Dimei XU %A Chenyuan ZHU %A Ke LU %A Xufang LIANG %J Journal of Zhejiang University SCIENCE B %P %@ 1673-1581 %D in press %I Zhejiang University Press & Springer doi="https://doi.org/10.1631/jzus.B2400246"
TY - JOUR T1 - Role of try and amy in the feeding, digestion, growth and development of the Japanese medaka (Oryzias latipes): insight from a comparative gene knockout study A1 - Xiaodan JIA A1 - Shulin TANG A1 - Hexiong FENG A1 - Dimei XU A1 - Chenyuan ZHU A1 - Ke LU A1 - Xufang LIANG J0 - Journal of Zhejiang University Science B SP - EP - %@ 1673-1581 Y1 - in press PB - Zhejiang University Press & Springer ER - doi="https://doi.org/10.1631/jzus.B2400246"
Abstract: Digestive enzymes of fish are critical to food digestion at the larval stage, but convincing evidence proving the function and necessity of the associated digestive enzymes remains lacking. In this study, we generated the trypsin gene (try) and amylase gene (amy) in the Japanese medaka (Oryzias latipes) using CRISPR for the first time. try deletion significantly decreased the expression of try and digestive capacity in try-/- medaka larvae; after 8 h digestion, incompletely digested brine shrimp was observed in the digestive tract at 4 and 15 days post-hatching (dph) of try-/- medaka larvae. Furthermore, the height of intestinal villi and total body length decreased significantly within 15 dph try-/- medaka larvae. However, amy deletion did not influence the digestion of medaka larvae at 4 dph. Only a small amount of incompletely digested brine shrimp was observed in 15-dph amy-/- medaka larvae. Further analysis of the growth, nitrogen metabolism, and intestinal microbes of try-/- adult medaka showed that the body length and weight of adult medaka decreased significantly, while the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the blood significantly increased. Pathological observation of liver and intestinal tissues showed that try knockout resulted in vacuolar degeneration of liver cells, thinning of the intestinal wall, sparse arrangement of villi, and lower villi height. High-throughput 16s rRNA sequencing revealed that try knockout reduced the diversity of intestinal microbes. These findings demonstrated that try was indispensable for medaka larvae because it continuously affects their growth, nitrogen metabolism, and intestinal development.
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