Accepted manuscript available online (unedited version)
An integrative combination of gas-liquid-phase plasma 4 mutagenesis and high-throughput screening to enhance 5 eicosapentaenoic acid production by Schizochytrium sp.
Affiliation(s):
University of Science and Technology of China, Hefei 230026, China;
moreAffiliation(s): University of Science and Technology of China, Hefei 230026, China; Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; Cabio Biotech (Wuhan) Biotechnology Co. Ltd., Wuhan 430223, China; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang 14 University, Hangzhou 310030, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China; Zhejiang Key Laboratory of Antifungal Drugs, Taizhou 318000, China;
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Chao YU, Jialin ZHU, Jinyong WU, Xiangsong CHEN, Shuhuan LU, Xiangyu LI, Sa ZHAO, Weiwei ZHU, Min SHU, Mianbin WU, Jianming YAO. An integrative combination of gas-liquid-phase plasma 4 mutagenesis and high-throughput screening to enhance 5 eicosapentaenoic acid production by Schizochytrium sp.[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400427
@article{title="An integrative combination of gas-liquid-phase plasma 4 mutagenesis and high-throughput screening to enhance 5 eicosapentaenoic acid production by Schizochytrium sp.", author="Chao YU, Jialin ZHU, Jinyong WU, Xiangsong CHEN, Shuhuan LU, Xiangyu LI, Sa ZHAO, Weiwei ZHU, Min SHU, Mianbin WU, Jianming YAO", journal="Journal of Zhejiang University Science B", year="in press", publisher="Zhejiang University Press & Springer", doi="https://doi.org/10.1631/jzus.B2400427" }
%0 Journal Article %T An integrative combination of gas-liquid-phase plasma 4 mutagenesis and high-throughput screening to enhance 5 eicosapentaenoic acid production by Schizochytrium sp. %A Chao YU %A Jialin ZHU %A Jinyong WU %A Xiangsong CHEN %A Shuhuan LU %A Xiangyu LI %A Sa ZHAO %A Weiwei ZHU %A Min SHU %A Mianbin WU %A Jianming YAO %J Journal of Zhejiang University SCIENCE B %P %@ 1673-1581 %D in press %I Zhejiang University Press & Springer doi="https://doi.org/10.1631/jzus.B2400427"
TY - JOUR T1 - An integrative combination of gas-liquid-phase plasma 4 mutagenesis and high-throughput screening to enhance 5 eicosapentaenoic acid production by Schizochytrium sp. A1 - Chao YU A1 - Jialin ZHU A1 - Jinyong WU A1 - Xiangsong CHEN A1 - Shuhuan LU A1 - Xiangyu LI A1 - Sa ZHAO A1 - Weiwei ZHU A1 - Min SHU A1 - Mianbin WU A1 - Jianming YAO J0 - Journal of Zhejiang University Science B SP - EP - %@ 1673-1581 Y1 - in press PB - Zhejiang University Press & Springer ER - doi="https://doi.org/10.1631/jzus.B2400427"
Abstract: Dietary consumption of eicosapentaenoic acid (EPA) offers diverse health benefits like regulation of blood triglycerides and prevention of cardiovascular diseases. EPA is naturally synthesized by Schizochytrium sp.; however, its low production level limit its potential for industrial application. The goal of this study was to increase EPA productivity in Schizochytrium sp. by gas-liquid-phase plasma mutagenesis (GLPP) combined with a high-throughput screening method. First, a diverse array of mutants was generated through GLPP mutagenesis. Next, the mutants with elevated EPA productivity were identified through near-infrared spectroscopy. Notably, the M7-25 mutant demonstrated the highest and most consistent EPA production. After the culture medium was optimized, the EPA titer increased from 0.45 to 1.70 g/L. Finally, a cofermentation strategy using ammonia and glucose feeding was employed, and the EPA titer reached 2.08 g/L in a 7 L fermenter. To date, this is the highest EPA titer reported in Schizochytrium sp. using mutagenesis technology, indicating its great market potential for industrial production.
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