JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model

Author(s): Yuxin ZHAO¹, Jia WANG¹, Dongqi YOU¹, Yifan LU¹, Mengfei YU¹, Misi SI¹
Affiliation(s): ¹Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, China
Corresponding email(s): misi_si@zju.edu.cn, yumengfei@zju.edu.cn
Key Words: Schneiderian membrane-derived mesenchymal stem cells; Single-cell RNA sequencing; Osteogenesis; Maxillary sinus floor elevation; Calvarial defect

Share this article to： More <<< Previous Paper \|Next Paper >>>

Yuxin ZHAO¹, Jia WANG¹, Dongqi YOU¹, Yifan LU¹, Mengfei YU¹, Misi SI¹. Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400611

@article{title="Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model",
author="Yuxin ZHAO¹, Jia WANG¹, Dongqi YOU¹, Yifan LU¹, Mengfei YU¹, Misi SI¹",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2400611"
}

%0 Journal Article
%T Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model
%A Yuxin ZHAO¹
%A Jia WANG¹
%A Dongqi YOU¹
%A Yifan LU¹
%A Mengfei YU¹
%A Misi SI¹
%J Journal of Zhejiang University SCIENCE B
%P
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2400611"

TY - JOUR
T1 - Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model
A1 - Yuxin ZHAO¹
A1 - Jia WANG¹
A1 - Dongqi YOU¹
A1 - Yifan LU¹
A1 - Mengfei YU¹
A1 - Misi SI¹
J0 - Journal of Zhejiang University Science B
SP -
EP -
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2400611"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Objectives: Schneiderian membrane-derived mesenchymal stem cells (SMMSCs) have been reported to be osteogenic progenitor cells in vitro. However, there is controversy regarding the intrinsic osteogenic capacity of the Schneiderian membrane, and the bone formation potential of SMMSCs in vivo has never been reported. Therefore, in this study we aimed to evaluate the contribution of the Schneiderian membrane to sinus floor elevation and to verify the function of SMMSCs in cranial bone defects. Materials and methods: Bilateral sinus floor elevation with chloromethyl-benzamidodialkylcarbocyanine (CM-DiI) labeling was performed in rabbits to assess Schneiderian membrane osteogenesis. Single-cell RNA sequencing was used to characterize human Schneiderian membrane cellular subsets. SMMSCs and bone marrow-derived mesenchymal stem cells (BMSCs) were transplanted into rabbit cranial defects with Gelatin Methacryloy (GelMA) scaffolds and analyzed via micro-CT and histology. Results: Spontaneous bone formation adjacent to the Schneiderian membrane was observed. Single-cell analysis identified paired related homeobox1 (PRRX1) progenitor clusters driving endo-sinus osteogenesis. SMMSCs exhibited superior earlier bone regeneration (4 weeks) compared with BMSCs, with higher tissue volume and bone volume/total volume (BV / TV) ratios. Conclusions: The Schneiderian membrane likely contributes to osteogenesis via PRRX1⁺ progenitor lineages. SMMSCs promote accelerated early bone regeneration in cranial defects. This study provides the first in vivo validation of the osteogenic capacity of SMMSCs and defines their molecular identity at single-cell resolution.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

Open peer comments: Debate/Discuss/Question/Opinion

<1>

- Go to

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference