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Received: 2021-09-17

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Haoru TANG


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Journal of Zhejiang University SCIENCE B

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Comparative metabolomics provides novel insights into the basis of petiole color differences in celery (Apium graveolens L.)

Author(s):  Mengyao LI, Jie LI, Haohan TAN, Ya LUO, Yong ZHANG, Qing CHEN, Yan WANG, Yuanxiu LIN, Yunting ZHANG, Xiaorong WANG, Haoru TANG

Affiliation(s):  College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China; more

Corresponding email(s):  htang@sicau.edu.cn

Key Words:  Celery; Metabolite; Anthocyanin; Chlorophyll; Petiole color

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Mengyao LI, Jie LI, Haohan TAN, Ya LUO, Yong ZHANG, Qing CHEN, Yan WANG, Yuanxiu LIN, Yunting ZHANG, Xiaorong WANG, Haoru TANG. Comparative metabolomics provides novel insights into the basis of petiole color differences in celery (Apium graveolens L.)[J]. Journal of Zhejiang University Science B, 2022, 23(3): 300-314.

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author="Mengyao LI, Jie LI, Haohan TAN, Ya LUO, Yong ZHANG, Qing CHEN, Yan WANG, Yuanxiu LIN, Yunting ZHANG, Xiaorong WANG, Haoru TANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Comparative metabolomics provides novel insights into the basis of petiole color differences in celery (Apium graveolens L.)
%A Mengyao LI
%A Jie LI
%A Haohan TAN
%A Qing CHEN
%A Yuanxiu LIN
%A Yunting ZHANG
%A Xiaorong WANG
%A Haoru TANG
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 4
%P 300-314
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer

T1 - Comparative metabolomics provides novel insights into the basis of petiole color differences in celery (Apium graveolens L.)
A1 - Mengyao LI
A1 - Jie LI
A1 - Haohan TAN
A1 - Ya LUO
A1 - Yong ZHANG
A1 - Qing CHEN
A1 - Yan WANG
A1 - Yuanxiu LIN
A1 - Yunting ZHANG
A1 - Xiaorong WANG
A1 - Haoru TANG
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 4
SP - 300
EP - 314
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -

Plant metabolites are important for plant development and human health. Plants of celery (Apium graveolens L.) with different-colored petioles have been formed in the course of long-term evolution. However, the composition, content distribution, and mechanisms of accumulation of metabolites in different-colored petioles remain elusive. Using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), 1159 metabolites, including 100 lipids, 72 organic acids and derivatives, 83 phenylpropanoids and polyketides, and several alkaloids and terpenoids, were quantified in four celery cultivars, each with a different petiole color. There were significant differences in the types and contents of metabolites in celery with different-colored petioles, with the most striking difference between green celery and purple celery, followed by white celery and green celery. Annotated analysis of metabolic pathways showed that the metabolites of the different-colored petioles were significantly enriched in biosynthetic pathways such as anthocyanin, flavonoid, and chlorophyll pathways, suggesting that these metabolic pathways may play a key role in determining petiole color in celery. The content of chlorophyll in green celery was significantly higher than that in other celery cultivars, yellow celery was rich in carotenoids, and the content of anthocyanin in purple celery was significantly higher than that in the other celery cultivars. The color of the celery petioles was significantly correlated with the content of related metabolites. Among the four celery cultivars, the metabolites of the anthocyanin biosynthesis pathway were enriched in purple celery. The results of quantitative real-time polymerase chain reaction (qRT-PCR) suggested that the differential expression of the chalcone synthase (CHS) gene in the anthocyanin biosynthesis pathway might affect the biosynthesis of anthocyanin in celery. In addition, HPLC analysis revealed that cyanidin is the main pigment in purple celery. This study explored the differences in the types and contents of metabolites in celery cultivars with different-colored petioles and identified key substances for color formation. The results provide a theoretical basis and technical support for genetic improvement of celery petiole color.




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