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Received: 2021-09-17

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 ORCID:

Yi ZHANG

https://orcid.org/0000-0002-1600-7456

Zhong-Ji QIAN

https://orcid.org/0000-0001-9220-2600

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A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells


Author(s):  Minqi CHEN, Jinyue LIANG, Yuan WANG, Yayue LIU, Chunxia ZHOU, Pengzhi HONG, Yi ZHANG, Zhong-Ji QIAN

Affiliation(s):  College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China; more

Corresponding email(s):  hubeizhangyi@163.com, zjqian78@163.com

Key Words:  Benzaldehydes; Aspergillus terreus C23-3; Anti-inflammation; MAPK signaling pathway; RAW264.7


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Minqi CHEN, Jinyue LIANG, Yuan WANG, Yayue LIU, Chunxia ZHOU, Pengzhi HONG, Yi ZHANG, Zhong-Ji QIAN. A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells[J]. Journal of Zhejiang University Science B, 2022, 23(5): 230-240.

@article{title="A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells",
author="Minqi CHEN, Jinyue LIANG, Yuan WANG, Yayue LIU, Chunxia ZHOU, Pengzhi HONG, Yi ZHANG, Zhong-Ji QIAN",
journal="Journal of Zhejiang University Science B",
volume="23",
number="3",
pages="230-240",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100807"
}

%0 Journal Article
%T A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells
%A Minqi CHEN
%A Jinyue LIANG
%A Yuan WANG
%A Yayue LIU
%A Chunxia ZHOU
%A Pengzhi HONG
%A Yi ZHANG
%A Zhong-Ji QIAN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 3
%P 230-240
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer

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T1 - A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells
A1 - Minqi CHEN
A1 - Jinyue LIANG
A1 - Yuan WANG
A1 - Yayue LIU
A1 - Chunxia ZHOU
A1 - Pengzhi HONG
A1 - Yi ZHANG
A1 - Zhong-Ji QIAN
J0 - Journal of Zhejiang University Science B
VL - 23
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Abstract: 
Marine fungi are important members of the marine microbiome, which have been paid growing attention by scientists in recent years. The secondary metabolites of marine fungi have been reported to contain rich and diverse compounds with novel structures (Chen et al., 2019). Aspergillus terreus, the higher level marine fungus of the Aspergillus genus (family of Trichocomaceae, order of Eurotiales, class of Eurotiomycetes, phylum of Ascomycota), is widely distributed in both sea and land. In our previous study, the coral-derived A. terreus strain C23-3 exhibited potential in producing other biologically active (with antioxidant, acetylcholinesterase inhibition, and anti-inflammatory activity) compounds like arylbutyrolactones, territrems, and isoflavones, and high sensitivity to the chemical regulation of secondary metabolism (Yang et al., 2019, 2020; Nie et al., 2020; Ma et al., 2021). Moreover, we have isolated two different benzaldehydes, including a benzaldehyde with a novel structure, from A. terreus C23-3 which was derived from Pectinia paeonia of Xuwen, Zhanjiang City, Guangdong Province, China.

珊瑚来源真菌土曲霉C23-3中的一种新苯甲醛通过抑制RAW264.7细胞中MAPK通路发挥抗炎作用

目的:研究从一株徐闻牡丹珊瑚来源的土曲霉C23-3的发酵液中所提取出来的新化合物(S)-3-(2,3-二羟基-3-甲基丁基)-4-羟基苯甲醛((S)-3-(2,3-dihydroxy-3-methylbutyl)-4-hydroxybenzaldehyde)(1)以及已知天然产物4-羟基-3-(3-甲基丁-2-烯-1-基)-苯甲醛(4-hydroxy-3-(3-methylbut-2-en-1-yl)-benzaldehyde)(2)的抗氧化和抗炎活性及其机制。
创新点:从一株徐闻牡丹珊瑚来源的土曲霉C23-3的发酵液中所提取出来了一种新化合物1及已知天然产物2,目前这两种苯甲醛的炎症相关研究尚未有报道。此外,这两种苯甲醛在未来的炎症相关疾病中有潜在的应用前景。
方法:通过一维(1D)、二维核磁共振谱(2D NMR)、高分辨电喷雾质谱(HR-ESI-MS)和旋光性分析,得到了一种新的化合物(S)-3-(2,3-二羟基-3-甲基丁基)-4-羟基苯甲醛。运用免疫印迹法、酶联免疫吸附试验(ELISA)、免疫荧光法和分子对接等方法,并通过研究一氧化氮(NO)、活性氧(ROS)、诱导型一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)、白细胞介素-6(IL-6)、丝裂原活化蛋白激酶(MAPK)和核因子-κB(NF-κB)信号通路的表达来探讨其抗炎作用。
结论:这两种苯甲醛具有显著的抗炎能力,是iNOS、COX-2、c-Jun氨基末端激酶(JNK)、细胞外调节蛋白激酶(ERK)和p38的潜在抑制剂。

关键词组:苯甲醛;土曲霉C23-3;抗炎;MAPK信号通路;RAW264.7

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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