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On-line Access: 2022-06-16
Received: 2021-12-07
Revision Accepted: 2022-05-04
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Bin ZHANG, Feng ZHANG, Fengying LU, Jing WANG, Wenbai ZHOU, Huihui WANG, Bin YU. Reduced cell invasion may be a characteristic of placental defects in pregnant women of advanced maternal age at single-cell level[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2101024 @article{title="Reduced cell invasion may be a characteristic of placental defects in pregnant women of advanced maternal age at single-cell level", %0 Journal Article TY - JOUR
从单细胞水平揭示细胞侵袭减少可能是高龄孕妇胎盘缺陷特征南京医科大学,常州医学中心,常州市妇幼保健院医学遗传科,中国常州市,213000 目的:目前高龄孕妇(advanced maternal age,AMA)妊娠风险增高的原因尚不清楚,本研究从单细胞水平解析AMA孕妇胎盘的细胞组成与转录组特征。 创新点:首次利用单细胞测序技术描述AMA孕妇胎盘的细胞图谱,探讨AMA孕妇滋养细胞及其细胞亚型的特征性变化。 方法:收集2名AMA和2名正常孕妇胎盘组织进行单细胞RNA测序(scRNA-seq)生物信息学分析。在此基础上,从6名孕妇(3名AMA孕妇和3名正常对照)胎盘分离培养滋养细胞,采用transwell实验观察细胞侵袭能力,同时观察SERPINE1的特异性表达。采用HTR8-S/Vneo细胞对SERPINE1在细胞侵袭中的作用进行初步研究。 结论:细胞浸润受损可能是AMA女性不良妊娠结局风险增加的原因。SERPINE1在EVT细胞中的异常表达似乎起着重要作用。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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