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Journal of Zhejiang University SCIENCE B

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Polyamines protect boar sperm from lipopolysaccharide-induced mitochondrial dysfunction and apoptosis via casein kinase 2 activation


Author(s):  Rongnan LI, Xiaodong WU, Xueni YOU, Zhendong ZHU, Ming GUO, Guochao HOU, Tianjiao LI, Yi ZHENG, Wenxian ZENG

Affiliation(s):  Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China

Corresponding email(s):  zengwenxian2015@126.com

Key Words:  Polyamines; Lipopolysaccharide; Sperm; Mitochondria; Casein kinase 2


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Rongnan LI, Xiaodong WU, Xueni YOU, Zhendong ZHU, Ming GUO, Guochao HOU, Tianjiao LI, Yi ZHENG, Wenxian ZENG. Polyamines protect boar sperm from lipopolysaccharide-induced mitochondrial dysfunction and apoptosis via casein kinase 2 activation[J]. Journal of Zhejiang University Science B, 1998, -1(3): .

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author="Rongnan LI, Xiaodong WU, Xueni YOU, Zhendong ZHU, Ming GUO, Guochao HOU, Tianjiao LI, Yi ZHENG, Wenxian ZENG",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200052"
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%T Polyamines protect boar sperm from lipopolysaccharide-induced mitochondrial dysfunction and apoptosis via casein kinase 2 activation
%A Rongnan LI
%A Xiaodong WU
%A Xueni YOU
%A Zhendong ZHU
%A Ming GUO
%A Guochao HOU
%A Tianjiao LI
%A Yi ZHENG
%A Wenxian ZENG
%J Journal of Zhejiang University SCIENCE B
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%@ 1673-1581
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%I Zhejiang University Press & Springer

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T1 - Polyamines protect boar sperm from lipopolysaccharide-induced mitochondrial dysfunction and apoptosis via casein kinase 2 activation
A1 - Rongnan LI
A1 - Xiaodong WU
A1 - Xueni YOU
A1 - Zhendong ZHU
A1 - Ming GUO
A1 - Guochao HOU
A1 - Tianjiao LI
A1 - Yi ZHENG
A1 - Wenxian ZENG
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VL - -1
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PB - Zhejiang University Press & Springer
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Abstract: 
Bacterial contamination is an inevitable issue in the routine collection and treatment procedures of boar semen. As a prototypical endotoxin from Gram-negative bacteria in the semen, lipopolysaccharide (LPS) undermines the structural and functional integrity of sperm. Spermine and spermidine could protect cells against LPS-induced injury, and the content of polyamines in seminal plasma is positively correlated with sperm quality. Thus, the goal of the present study was to elucidate whether the addition of polyamines is beneficial to boar semen preservation and able to prevent LPS-induced sperm damage. The addition of polyamines in the extender resulted in higher sperm motility, plasma-membrane integrity, acrosome integrity, and mitochondrial membrane potential (ΔΨm) after preservation in vitro at 17 °C for 7 d (P < 0.05). LPS induced sperm-quality decrease, ΔΨm decline, cellular adenosine-triphosphate (ATP) depletion, abnormal mitochondrial ultrastructure, mitochondrial permeability transition pore (MPTP) opening, phosphatidylserine (PS) translocation, and caspase-3 activation (P < 0.05). Interestingly, polyamines alleviated the LPS-induced changes of the aforementioned parameters, and mitigated the decrease in the ratio of microtubule-associated protein light chain 3-II (LC3-II) to LC3-I. Meanwhile, the α and β subunits of casein kinase 2 (CK2) were detected at the connecting piece and the tail. Importantly, the addition of 4,5,6,7-tetrabromobenzotriazole (TBB), a specific CK2 inhibitor, counteracted the positive effects of polyamines on sperm quality, mitochondrial activity, and decrease of apoptotic protein level after incubation at 37 °C for 6 h. Together, these results suggest that polyamines prevent LPS-induced mitochondrial dysfunction and apoptosis through CK2 activation, which contributes to the maintenance of sperm structural and functional integrity from LPS-induced injury, thereby improving sperm quality and the efficacy of semen preservation.

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