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On-line Access: 2022-11-15
Received: 2022-02-10
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Citations: Bibtex RefMan EndNote GB/T7714
Hongdan GAO, Zifang SHANG, Siew Yin CHAN, Dongli MA. Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200068 @article{title="Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens", %0 Journal Article TY - JOUR
CRISPR-CAS系统检测传染病病原体的研究进展1深圳市儿童医院儿科研究所,中国深圳,518026 2中国科学院微生物研究所病原微生物学重点实验室,中国北京,100101 3西北工业大学柔性电子研究院,中国西安,710072 概要:感染性疾病对个人乃至社会均可造成极大的经济损失和精神痛苦。现有检测方法在灵敏度与特异性、检测时间、昂贵仪器设备依赖方面或多或少存在不足。成簇的规律间隔的短回文重复序列(CRISPR)-相关蛋白(Cas)系统,因其具备特异、灵敏、快速、便捷等优势,近年来被尝试应用于感染性疾病病原体的检测。本文首先回顾了CRISPR-Cas系统的发现发展进程,并列举可与之结合获得检测结果的检测结果阅读方式,之后从检测过程中所应用的不同Cas蛋白的角度简要描述了目前已有的基于CRISRP检测感染性疾病病原体的应用,最后还展望了CRISPR/Cas系统未来在检测方面的应用前景及挑战。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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