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On-line Access: 2022-11-15

Received: 2022-02-10

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Zifang SHANG


Dongli MA


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Journal of Zhejiang University SCIENCE B

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Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens

Author(s):  Hongdan GAO, Zifang SHANG, Siew Yin CHAN, Dongli MA

Affiliation(s):  Institute of Pediatrics, Shenzhen Children’s Hospital, Shenzhen 518026, China; more

Corresponding email(s):  madl1234@126.com

Key Words:  CRISPR-Cas; Diagnosis; Disease detection; Infectious disease; Pathogen

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Hongdan GAO, Zifang SHANG, Siew Yin CHAN, Dongli MA. Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens[J]. Journal of Zhejiang University Science B, 2022, 23(1): 881-898.

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%T Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens
%A Hongdan GAO
%A Zifang SHANG
%A Siew Yin CHAN
%A Dongli MA
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%I Zhejiang University Press & Springer

T1 - Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens
A1 - Hongdan GAO
A1 - Zifang SHANG
A1 - Siew Yin CHAN
A1 - Dongli MA
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 11
SP - 881
EP - 898
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -

Infectious diseases cause great economic loss and individual and even social anguish. Existing detection methods lack sensitivity and specificity, have a poor turnaround time, and are dependent on expensive equipment. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR)‍-CRISPR-associated protein (Cas) system has been widely used in the detection of pathogens that cause infectious diseases owing to its high specificity, sensitivity, and speed, and good accessibility. In this review, we discuss the discovery and development of the CRISPR-Cas system, summarize related analysis and interpretation methods, and discuss the existing applications of CRISPR-based detection of infectious pathogens using Cas proteins. We conclude the challenges and prospects of the CRISPR-Cas system in the detection of pathogens.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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