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Journal of Zhejiang University SCIENCE B

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Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens


Author(s):  Hongdan GAO, Zifang SHANG, Siew Yin CHAN, Dongli MA

Affiliation(s):  Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen 518026, China; more

Corresponding email(s):  shangzifang@foxmail.com, madl1234@126.com

Key Words:  CRISPR-Cas; Diagnosis; Disease Detection; Infectious disease; Pathogen


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Hongdan GAO, Zifang SHANG, Siew Yin CHAN, Dongli MA. Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens[J]. Journal of Zhejiang University Science B, 1998, -1(3): .

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Abstract: 
Infectious diseases cause great economic loss and individual and even social anguish. Existing detection methods lack sensitivity and specificity, have a poor turnaround time and are dependent on expensive equipment. In recent years the clustered regularly interspaced short palindromic repeats-associated proteins (CRISPR-Cas) system has been widely used in the detection of pathogens that cause infectious diseases owing to its high specificity and sensitivity, speed, and good accessibility. In this review, we discuss the discovery and development of the CRISPR-Cas system, summarize related analysis and interpretation methods, and discuss the existing applications of CRISPR-based detection of infectious pathogens using Cas proteins. We conclude by listing the challenges and prospects of the CRISPR-Cas system in the detection of pathogens.

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