CLC number:
On-line Access: 2023-01-10
Received: 2022-04-08
Revision Accepted: 2022-07-13
Crosschecked: 2023-01-16
Cited: 0
Clicked: 930
Citations: Bibtex RefMan EndNote GB/T7714
Kang XU, Xiyun JIN, Ya LUO, Haozhe ZOU, Dezhong LV, Liping WANG, Limei FU, Yangyang CAI, Tingting SHAO, Yongsheng LI, Juan XU. Spatial transcriptome analysis of long non-coding RNAs reveals tissue specificity and functional roles in cancer[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200206 @article{title="Spatial transcriptome analysis of long non-coding RNAs reveals tissue specificity and functional roles in cancer", %0 Journal Article TY - JOUR
长链非编码RNA空间转录组分析揭示组织特异性和癌症中的功能1哈尔滨医科大学生物信息学科学与技术学院,中国哈尔滨市,150081 2海南医学院热带转化医学教育部重点实验室,生物医学信息与工程学院,海南省妇女儿童医学中心,中国海口市,571199 目的:长链非编码RNA(long non-coding RNAs,lncRNAs)在维持组织形态和功能方面发挥着重要作用,其精确调控效应与其表达模式密切相关。然而,lncRNA在人体内的空间表达模式还未得到明确刻画。 方法:在本研究中,我们构建了5个完整的人类lncRNAs转录组图谱,覆盖了数千个正常或疾病状态下的主要组织样本。在不同资源的相同组织中,lncRNA转录组表现出高度的一致性,在特定组织中则表现出更高的复杂性。在每个资源中都发现了组织升高(tissue-elevated,TE)的lncRNA,并通过整合分析提炼出了稳定的TE lncRNA。我们在组织中检测到了1~4684个稳定的TE lncRNAs,其中TE lncRNA最多的是睾丸组织,其次是脑组织。TE lncRNA的功能分析表明,TE lncRNA在相应的组织相关通路中发挥重要作用。此外,我们发现,稳健的TE lncRNA的表达特征使其成为区分组织的有效生物标记物;TE lncRNA也倾向于与癌症相关,表现出差异表达或与患者生存相关。 结论:综上所述,lncRNAs的空间分类是阐明lncRNAs在维持组织形态和组织限制性疾病进展中功能的重要起点。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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